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[The diagnostic significance of particular immune-dominating proteins of isogenic variants of Bacillus Anthracis.]
Klin Lab Diagn 2016; 61(12):833-837KL

Abstract

The polyclonal mono-specific sera to proteins of toxin and antigens of S-layer of Bacillus Anthracis separated in preparative electrophoresis. While fluorescent antibody technique was applied, immunoglobulins of sera to protein of S-layer of Bacillus Anthracis m.m. 94 kDa had specificity and specific activity in presented number of strains. In the soil samples spores in concentration of 1x104 KOE/ml were detected that permits to recommend the given immunoglobulins for indexation of Bacillus Anthracis. The proteins m.m. 90 kDa relevant to antigens of toxin of Bacillus Anthracis reacted in immuno-blotting with sera from patients with skin form of anthrax and guinea pigs immunized and survived after infection. Thew sera to them are species specified and can be applied for diagnostic of disease (detection of protective antigen) and detection of production of proteins in reaction of immunodiffusion with growing cultures.

Authors+Show Affiliations

The Volgogradskii anti-plague research institute of Rospotrebnadzor, 400131, Volgograd, Russia.The Volgogradskii anti-plague research institute of Rospotrebnadzor, 400131, Volgograd, Russia.The Volgogradskii anti-plague research institute of Rospotrebnadzor, 400131, Volgograd, Russia.The Volgogradskii anti-plague research institute of Rospotrebnadzor, 400131, Volgograd, Russia.The Volgogradskii anti-plague research institute of Rospotrebnadzor, 400131, Volgograd, Russia.

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

31536695

Citation

Barkova, I A., et al. "[The Diagnostic Significance of Particular Immune-dominating Proteins of Isogenic Variants of Bacillus Anthracis.]." Klinicheskaia Laboratornaia Diagnostika, vol. 61, no. 12, 2016, pp. 833-837.
Barkova IA, Chervakova MP, Barkov AM, et al. [The diagnostic significance of particular immune-dominating proteins of isogenic variants of Bacillus Anthracis.]. Klin Lab Diagn. 2016;61(12):833-837.
Barkova, I. A., Chervakova, M. P., Barkov, A. M., Novojenina, A. V., & Viktorov, D. V. (2016). [The diagnostic significance of particular immune-dominating proteins of isogenic variants of Bacillus Anthracis.]. Klinicheskaia Laboratornaia Diagnostika, 61(12), pp. 833-837. doi:10.18821/0869-2084-2016-61-12-833-837.
Barkova IA, et al. [The Diagnostic Significance of Particular Immune-dominating Proteins of Isogenic Variants of Bacillus Anthracis.]. Klin Lab Diagn. 2016;61(12):833-837. PubMed PMID: 31536695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The diagnostic significance of particular immune-dominating proteins of isogenic variants of Bacillus Anthracis.] AU - Barkova,I A, AU - Chervakova,M P, AU - Barkov,A M, AU - Novojenina,A V, AU - Viktorov,D V, PY - 2016/03/17/received PY - 2016/03/21/accepted PY - 2019/9/20/entrez PY - 2016/1/1/pubmed PY - 2016/1/1/medline KW - cultural filtrates KW - fluorescent antibody technique KW - preparative electrophoresis KW - proteins m.m. 94, 87, 90 kDa of Bacillus Anthracis SP - 833 EP - 837 JF - Klinicheskaia laboratornaia diagnostika JO - Klin. Lab. Diagn. VL - 61 IS - 12 N2 - The polyclonal mono-specific sera to proteins of toxin and antigens of S-layer of Bacillus Anthracis separated in preparative electrophoresis. While fluorescent antibody technique was applied, immunoglobulins of sera to protein of S-layer of Bacillus Anthracis m.m. 94 kDa had specificity and specific activity in presented number of strains. In the soil samples spores in concentration of 1x104 KOE/ml were detected that permits to recommend the given immunoglobulins for indexation of Bacillus Anthracis. The proteins m.m. 90 kDa relevant to antigens of toxin of Bacillus Anthracis reacted in immuno-blotting with sera from patients with skin form of anthrax and guinea pigs immunized and survived after infection. Thew sera to them are species specified and can be applied for diagnostic of disease (detection of protective antigen) and detection of production of proteins in reaction of immunodiffusion with growing cultures. SN - 0869-2084 UR - https://www.unboundmedicine.com/medline/citation/31536695/[The_diagnostic_significance_of_particular_immune-dominating_proteins_of_isogenic_variants_of_Bacillus_Anthracis.] DB - PRIME DP - Unbound Medicine ER -