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Perfused murine heart optical transmission spectroscopy using optical catheter and integrating sphere: Effects of ischemia/reperfusion.
Anal Biochem 2019; 586:113443AB

Abstract

Tissue transmission optical absorption spectroscopy provides dynamic information on metabolism and function. Murine genetic malleability makes it a major model for heart research. The diminutive size of the mouse heart makes optical transmission studies challenging. Using a perfused murine heart center mounted in an integrating sphere for light collection with a ventricular cavity optical catheter as an internal light source provided an effective method of optical data collection in this model. This approach provided high signal to noise optical spectra which when fit with model spectra provided information on tissue oxygenation and redox state. This technique was applied to the study of cardiac ischemia and ischemia reperfusion which generates extreme heart motion, especially during the ischemic contracture. The integrating sphere reduced motion artifacts associated with a fixed optical pickup and methods were developed to compensate for changes in tissue thickness. During ischemia, rapid decreases in myoglobin oxygenation occurred along with increases in cytochrome reduction levels. Surprisingly, when ischemic contracture occurred, myoglobin remained fully deoxygenated, while the cytochromes became more reduced consistent with a further, and critical, reduction of mitochondrial oxygen tension during ischemic contraction. This optical arrangement is an effective method of monitoring murine heart metabolism.

Authors+Show Affiliations

Laboratory of Cardiac Physiology, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Tyler.Bauer@nih.gov.Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Abigail.Giles@nih.gov.Laboratory of Cardiac Physiology, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Junhui.sun@nih.gov.Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Armel.Femnou@nih.gov.Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Raul.coviangarcia@nih.gov.Laboratory of Cardiac Physiology, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: muphy1@mail.nih.gov.Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, Bethesda, MD, USA. Electronic address: Balabanr@nhlbi.nih.gov.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31539522

Citation

Bauer, Tyler M., et al. "Perfused Murine Heart Optical Transmission Spectroscopy Using Optical Catheter and Integrating Sphere: Effects of Ischemia/reperfusion." Analytical Biochemistry, vol. 586, 2019, p. 113443.
Bauer TM, Giles AV, Sun J, et al. Perfused murine heart optical transmission spectroscopy using optical catheter and integrating sphere: Effects of ischemia/reperfusion. Anal Biochem. 2019;586:113443.
Bauer, T. M., Giles, A. V., Sun, J., Femnou, A., Covian, R., Murphy, E., & Balaban, R. S. (2019). Perfused murine heart optical transmission spectroscopy using optical catheter and integrating sphere: Effects of ischemia/reperfusion. Analytical Biochemistry, 586, p. 113443. doi:10.1016/j.ab.2019.113443.
Bauer TM, et al. Perfused Murine Heart Optical Transmission Spectroscopy Using Optical Catheter and Integrating Sphere: Effects of Ischemia/reperfusion. Anal Biochem. 2019 Dec 1;586:113443. PubMed PMID: 31539522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Perfused murine heart optical transmission spectroscopy using optical catheter and integrating sphere: Effects of ischemia/reperfusion. AU - Bauer,Tyler M, AU - Giles,Abigail V, AU - Sun,Junhui, AU - Femnou,Armel, AU - Covian,Raul, AU - Murphy,Elizabeth, AU - Balaban,Robert S, Y1 - 2019/09/17/ PY - 2019/07/25/received PY - 2019/09/16/accepted PY - 2019/9/21/pubmed PY - 2019/9/21/medline PY - 2019/9/21/entrez KW - Cytochromes KW - Linear least squares fitting KW - Mitochondria membrane potential KW - Myoglobin KW - Optical pathlength KW - Oxidative phosphorylation KW - Oxygen SP - 113443 EP - 113443 JF - Analytical biochemistry JO - Anal. Biochem. VL - 586 N2 - Tissue transmission optical absorption spectroscopy provides dynamic information on metabolism and function. Murine genetic malleability makes it a major model for heart research. The diminutive size of the mouse heart makes optical transmission studies challenging. Using a perfused murine heart center mounted in an integrating sphere for light collection with a ventricular cavity optical catheter as an internal light source provided an effective method of optical data collection in this model. This approach provided high signal to noise optical spectra which when fit with model spectra provided information on tissue oxygenation and redox state. This technique was applied to the study of cardiac ischemia and ischemia reperfusion which generates extreme heart motion, especially during the ischemic contracture. The integrating sphere reduced motion artifacts associated with a fixed optical pickup and methods were developed to compensate for changes in tissue thickness. During ischemia, rapid decreases in myoglobin oxygenation occurred along with increases in cytochrome reduction levels. Surprisingly, when ischemic contracture occurred, myoglobin remained fully deoxygenated, while the cytochromes became more reduced consistent with a further, and critical, reduction of mitochondrial oxygen tension during ischemic contraction. This optical arrangement is an effective method of monitoring murine heart metabolism. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/31539522/Perfused_murine_heart_optical_transmission_spectroscopy_using_optical_catheter_and_integrating_sphere:_Effects_of_ischemia/reperfusion L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(19)30733-X DB - PRIME DP - Unbound Medicine ER -