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Action of Bacillus thuringiensis (Bacillales: Bacillaceae) in the midgut of the sugarcane borer Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae).
Ecotoxicol Environ Saf 2019; 184:109642EE

Abstract

The relevance of Bacillus thuringiensis in pest control strategies have been increasing in recent decades. This entomopathogenic microorganism is considered safe, and its action is highly selective to target organisms. Its toxic effect is attributed to the toxins synthesized during sporulation. The nature of the produced toxins depends on the bacterial variety. Some varieties of B. thuringiensis can synthesize from one to eight distinct toxins, which have specific effects on different orders of susceptible insects. The sugarcane borer, Diatraea saccharalis, is a holometabolous lepidopteran that causes severe damage to sugar cane cultures during its larval phase. Therefore, to evaluate B. thuringiensis serovar Aizawai GC-91 efficiency and its effects on the midgut of first instar D. saccharalis larvae, we reared the insects with contaminated artificial diet for 144 h. Larvae mortality during this period revealed that 5, 10, and 20 g/L concentrations were the most efficient, resulting in 100% mortality of treated larvae, with LC50 and LC90 estimated at 0.307 and 1.330 g/L, respectively. Furthermore, histological and ultrastructural analyses revealed damage to midgut cells. Within the first 24 h of treatment, the midgut of infected insects presented peritrophic membrane degeneration, cytoplasmic vacuolization, and cellular hypertrophy; spherites and calcium granules were also observed. At 48 h of treatment, the digestive system had collapsed, with microvilli degeneration, basement membrane and muscular fiber bundles disruption, and cellular lysis. These results confirm the toxicity of the B. thuringiensis serovar Aizawai GC-91 on D. saccharalis larvae and its potential use as a biocontroller against this pest species.

Authors+Show Affiliations

Laboratory of Biological Control, Morphology and Insect Cytogenetics, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá - UEM, Maringá, PR, 87020-900, Brazil. Electronic address: bv.ds@hotmail.com.Laboratory of Insects, Department of Morphology, Institute of Biosciences of Botucatu, São Paulo State University - UNESP, Botucatu, SP, 18618-689, Brazil.Microscopy Center, Complex of Research Support Centers of the State University of Maringá - UEM, Maringá, PR, 87020-900, Brazil.Laboratory of Animal Genetics, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá - UEM, Maringá, PR, 87020-900, Brazil.Laboratory of Microbiology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá - UEM, Maringá, PR, 87020-900, Brazil.Laboratory of Biological Control, Morphology and Insect Cytogenetics, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá - UEM, Maringá, PR, 87020-900, Brazil. Electronic address: hconte@uem.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31539808

Citation

Daquila, Bruno Vinicius, et al. "Action of Bacillus Thuringiensis (Bacillales: Bacillaceae) in the Midgut of the Sugarcane Borer Diatraea Saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae)." Ecotoxicology and Environmental Safety, vol. 184, 2019, p. 109642.
Daquila BV, Scudeler EL, Dossi FCA, et al. Action of Bacillus thuringiensis (Bacillales: Bacillaceae) in the midgut of the sugarcane borer Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae). Ecotoxicol Environ Saf. 2019;184:109642.
Daquila, B. V., Scudeler, E. L., Dossi, F. C. A., Moreira, D. R., Pamphile, J. A., & Conte, H. (2019). Action of Bacillus thuringiensis (Bacillales: Bacillaceae) in the midgut of the sugarcane borer Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae). Ecotoxicology and Environmental Safety, 184, p. 109642. doi:10.1016/j.ecoenv.2019.109642.
Daquila BV, et al. Action of Bacillus Thuringiensis (Bacillales: Bacillaceae) in the Midgut of the Sugarcane Borer Diatraea Saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae). Ecotoxicol Environ Saf. 2019 Nov 30;184:109642. PubMed PMID: 31539808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Action of Bacillus thuringiensis (Bacillales: Bacillaceae) in the midgut of the sugarcane borer Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae). AU - Daquila,Bruno Vinicius, AU - Scudeler,Elton Luiz, AU - Dossi,Fábio Cleisto Alda, AU - Moreira,Daiani Rodrigues, AU - Pamphile,João Alencar, AU - Conte,Helio, Y1 - 2019/09/17/ PY - 2019/04/12/received PY - 2019/08/07/revised PY - 2019/09/03/accepted PY - 2019/9/21/pubmed PY - 2019/9/21/medline PY - 2019/9/21/entrez KW - Biotechnology KW - Cytotoxicity KW - Endotoxin KW - Entomopathogen KW - Histopathology KW - Sustainability SP - 109642 EP - 109642 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 184 N2 - The relevance of Bacillus thuringiensis in pest control strategies have been increasing in recent decades. This entomopathogenic microorganism is considered safe, and its action is highly selective to target organisms. Its toxic effect is attributed to the toxins synthesized during sporulation. The nature of the produced toxins depends on the bacterial variety. Some varieties of B. thuringiensis can synthesize from one to eight distinct toxins, which have specific effects on different orders of susceptible insects. The sugarcane borer, Diatraea saccharalis, is a holometabolous lepidopteran that causes severe damage to sugar cane cultures during its larval phase. Therefore, to evaluate B. thuringiensis serovar Aizawai GC-91 efficiency and its effects on the midgut of first instar D. saccharalis larvae, we reared the insects with contaminated artificial diet for 144 h. Larvae mortality during this period revealed that 5, 10, and 20 g/L concentrations were the most efficient, resulting in 100% mortality of treated larvae, with LC50 and LC90 estimated at 0.307 and 1.330 g/L, respectively. Furthermore, histological and ultrastructural analyses revealed damage to midgut cells. Within the first 24 h of treatment, the midgut of infected insects presented peritrophic membrane degeneration, cytoplasmic vacuolization, and cellular hypertrophy; spherites and calcium granules were also observed. At 48 h of treatment, the digestive system had collapsed, with microvilli degeneration, basement membrane and muscular fiber bundles disruption, and cellular lysis. These results confirm the toxicity of the B. thuringiensis serovar Aizawai GC-91 on D. saccharalis larvae and its potential use as a biocontroller against this pest species. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/31539808/Action_of_Bacillus_thuringiensis_(Bacillales:_Bacillaceae)_in_the_midgut_of_the_sugarcane_borer_Diatraea_saccharalis_(Fabricius,_1794)_(Lepidoptera:_Crambidae) L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(19)30973-X DB - PRIME DP - Unbound Medicine ER -