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Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used against Skin Diseases.
Plants (Basel). 2019 Sep 15; 8(9)P

Abstract

Although orthodox medications are available for skin diseases, expensive dermatological services have necessitated the use of medicinal plants as a cheaper alternative. This study evaluated the pharmacological and phytochemical profiles of four medicinal plants (Drimia sanguinea, Elephantorrhiza elephantina, Helichrysum paronychioides, and Senecio longiflorus) used for treating skin diseases. Petroleum ether and 50% methanol extracts of the plants were screened for antimicrobial activity against six microbes: Bacillus cereus, Shigella flexneri, Candida glabrata, Candida krusei, Trichophyton rubrum and Trichophyton tonsurans using the micro-dilution technique. Antioxidant activity was conducted using 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging and β-carotene linoleic acid models. Cytotoxicity was determined against African green monkey Vero kidney cells based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Spectrophotometric and Gas Chromatography-Mass Spectrometry (GC-MS) methods were used to evaluate the phytochemical constituents. All the extracts demonstrated varying degrees of antimicrobial potencies. Shigella flexneri, Candida glabrata, Trichophyton rubrum and Trichophyton tonsurans were most susceptible at 0.10 mg/mL. In the DPPH test, EC50 values ranged from approximately 6-93 µg/mL and 65%-85% antioxidant activity in the β-carotene linoleic acid antioxidant activity model. The phenolic and flavonoid contents ranged from 3.5-64 mg GAE/g and 1.25-28 mg CE/g DW, respectively. The LC50 values of the cytotoxicity assay ranged from 0.015-5622 µg/mL. GC-MS analysis revealed a rich pool (94-198) of bioactive compounds including dotriacontane, benzothiazole, heptacosane, bumetrizole, phthalic acid, stigmasterol, hexanoic acid and eicosanoic acid, which were common to the four plants. The current findings provide some degree of scientific evidence supporting the use of these four plants in folk medicine. However, the plants with high cytotoxicity need to be used with caution.

Authors+Show Affiliations

Indigenous Knowledge Systems (IKS) Centre, Faculty of Natural and Agricultural Sciences, North West University, Private Bag X2046, Mmabatho 2735, South Africa. johnmilan058@gmail.com.Agricultural Research Council, Vegetables and Ornamental Plants, Pretoria, Private Bag X293, Pretoria 0001, South Africa. AmooS@arc.agric.za.Phytomedicine Programme, Department of Paraclinical Sciences, University of Pretoria, Private Bag X04, Onderstepoort, Pretoria 0110, South Africa. lyndy.mcgaw@up.ac.za.Phytomedicine Programme, Department of Paraclinical Sciences, University of Pretoria, Private Bag X04, Onderstepoort, Pretoria 0110, South Africa.Indigenous Knowledge Systems (IKS) Centre, Faculty of Natural and Agricultural Sciences, North West University, Private Bag X2046, Mmabatho 2735, South Africa. Oladapo.aremu@nwu.ac.za. Food Security and Safety Niche Area, Faculty of Natural and Agricultural Sciences, North West University, Private Bag X2046, Mmabatho 2735, South Africa. Oladapo.aremu@nwu.ac.za.School of Biology and Environmental Sciences, Faculty of Agriculture & Natural sciences, University of Mpumalanga, Private Bag X11283, Mbombela 1200, South Africa. Wilfred.mbeng@ump.ac.za.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31540194

Citation

Asong, John A., et al. "Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used Against Skin Diseases." Plants (Basel, Switzerland), vol. 8, no. 9, 2019.
Asong JA, Amoo SO, McGaw LJ, et al. Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used against Skin Diseases. Plants (Basel). 2019;8(9).
Asong, J. A., Amoo, S. O., McGaw, L. J., Nkadimeng, S. M., Aremu, A. O., & Otang-Mbeng, W. (2019). Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used against Skin Diseases. Plants (Basel, Switzerland), 8(9). https://doi.org/10.3390/plants8090350
Asong JA, et al. Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used Against Skin Diseases. Plants (Basel). 2019 Sep 15;8(9) PubMed PMID: 31540194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial Activity, Antioxidant Potential, Cytotoxicity and Phytochemical Profiling of Four Plants Locally Used against Skin Diseases. AU - Asong,John A, AU - Amoo,Stephen O, AU - McGaw,Lyndy J, AU - Nkadimeng,Sanah M, AU - Aremu,Adeyemi O, AU - Otang-Mbeng,Wilfred, Y1 - 2019/09/15/ PY - 2019/08/26/received PY - 2019/09/02/revised PY - 2019/09/03/accepted PY - 2019/9/22/entrez PY - 2019/9/22/pubmed PY - 2019/9/22/medline KW - antibacterial KW - antifungal KW - antioxidant KW - flavonoids KW - gas chromatography-mass spectrometry (GC-MS) KW - minimum inhibitory concentration KW - phenols JF - Plants (Basel, Switzerland) JO - Plants (Basel) VL - 8 IS - 9 N2 - Although orthodox medications are available for skin diseases, expensive dermatological services have necessitated the use of medicinal plants as a cheaper alternative. This study evaluated the pharmacological and phytochemical profiles of four medicinal plants (Drimia sanguinea, Elephantorrhiza elephantina, Helichrysum paronychioides, and Senecio longiflorus) used for treating skin diseases. Petroleum ether and 50% methanol extracts of the plants were screened for antimicrobial activity against six microbes: Bacillus cereus, Shigella flexneri, Candida glabrata, Candida krusei, Trichophyton rubrum and Trichophyton tonsurans using the micro-dilution technique. Antioxidant activity was conducted using 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging and β-carotene linoleic acid models. Cytotoxicity was determined against African green monkey Vero kidney cells based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Spectrophotometric and Gas Chromatography-Mass Spectrometry (GC-MS) methods were used to evaluate the phytochemical constituents. All the extracts demonstrated varying degrees of antimicrobial potencies. Shigella flexneri, Candida glabrata, Trichophyton rubrum and Trichophyton tonsurans were most susceptible at 0.10 mg/mL. In the DPPH test, EC50 values ranged from approximately 6-93 µg/mL and 65%-85% antioxidant activity in the β-carotene linoleic acid antioxidant activity model. The phenolic and flavonoid contents ranged from 3.5-64 mg GAE/g and 1.25-28 mg CE/g DW, respectively. The LC50 values of the cytotoxicity assay ranged from 0.015-5622 µg/mL. GC-MS analysis revealed a rich pool (94-198) of bioactive compounds including dotriacontane, benzothiazole, heptacosane, bumetrizole, phthalic acid, stigmasterol, hexanoic acid and eicosanoic acid, which were common to the four plants. The current findings provide some degree of scientific evidence supporting the use of these four plants in folk medicine. However, the plants with high cytotoxicity need to be used with caution. SN - 2223-7747 UR - https://www.unboundmedicine.com/medline/citation/31540194/Antimicrobial_Activity_Antioxidant_Potential_Cytotoxicity_and_Phytochemical_Profiling_of_Four_Plants_Locally_Used_against_Skin_Diseases_ L2 - https://www.mdpi.com/resolver?pii=plants8090350 DB - PRIME DP - Unbound Medicine ER -
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