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Antimicrobial activity and mechanism of action of a novel peptide present in the ecdysis process of centipede Scolopendra subspinipes subspinipes.
Sci Rep 2019; 9(1):13631SR

Abstract

One of the most important cellular events in arthropods is the moulting of the cuticle (ecdysis). This process allows them to grow until they reach sexual maturity. Nevertheless, during this stage, the animals are highly exposed to pathogens. Consequently, it can be assumed that arthropods counter with an efficient anti-infective strategy that facilitates their survival during ecdysis. Herein, we characterized a novel antimicrobial peptide called Pinipesin, present in the exuviae extract of the centipede Scolopendra subspinipes subspinipes. The antimicrobial activity of Pinipesin was tested. The haemolytic activity of the peptide was evaluated and its possible mechanism of action was investigated. Identification was carried out by mass spectrometry analysis. Pinipesin displayed potent antimicrobial effects against different microorganisms and showed low haemolytic effects against human erythrocytes at high concentrations. It has a monoisotopic mass of 1213.57 Da, its sequence exhibited high similarity with some cuticular proteins, and it might act intracellularly by interfering with protein synthesis. Our data suggest that Pinipesin might be part of a prophylactic immune response during the ecdysis process of centipedes. Therefore, it is a promising candidate for the development of non-conventional antibiotics that could help fight infectious diseases and represents an exciting discovery for this taxon.

Authors+Show Affiliations

Special Laboratory for Applied Toxinology (LETA), Butantan Institute, São Paulo, 05503-900, Brazil.Special Laboratory for Applied Toxinology (LETA), Butantan Institute, São Paulo, 05503-900, Brazil. paulasegura64@gmail.com. Biomedical Science Institute, University of São Paulo, São Paulo, 05508-000, Brazil. paulasegura64@gmail.com.Department of Biophysics, Federal University of São Paulo, São Paulo, 04044-020, Brazil.Department of Biophysics, Federal University of São Paulo, São Paulo, 04044-020, Brazil.Department of Biophysics, Federal University of São Paulo, São Paulo, 04044-020, Brazil.Special Laboratory for Applied Toxinology (LETA), Butantan Institute, São Paulo, 05503-900, Brazil. Biomedical Science Institute, University of São Paulo, São Paulo, 05508-000, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31541146

Citation

Chaparro-Aguirre, Elisa, et al. "Antimicrobial Activity and Mechanism of Action of a Novel Peptide Present in the Ecdysis Process of Centipede Scolopendra Subspinipes Subspinipes." Scientific Reports, vol. 9, no. 1, 2019, p. 13631.
Chaparro-Aguirre E, Segura-Ramírez PJ, Alves FL, et al. Antimicrobial activity and mechanism of action of a novel peptide present in the ecdysis process of centipede Scolopendra subspinipes subspinipes. Sci Rep. 2019;9(1):13631.
Chaparro-Aguirre, E., Segura-Ramírez, P. J., Alves, F. L., Riske, K. A., Miranda, A., & Silva Júnior, P. I. (2019). Antimicrobial activity and mechanism of action of a novel peptide present in the ecdysis process of centipede Scolopendra subspinipes subspinipes. Scientific Reports, 9(1), p. 13631. doi:10.1038/s41598-019-50061-y.
Chaparro-Aguirre E, et al. Antimicrobial Activity and Mechanism of Action of a Novel Peptide Present in the Ecdysis Process of Centipede Scolopendra Subspinipes Subspinipes. Sci Rep. 2019 Sep 20;9(1):13631. PubMed PMID: 31541146.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial activity and mechanism of action of a novel peptide present in the ecdysis process of centipede Scolopendra subspinipes subspinipes. AU - Chaparro-Aguirre,Elisa, AU - Segura-Ramírez,Paula J, AU - Alves,Flavio L, AU - Riske,Karin A, AU - Miranda,Antonio, AU - Silva Júnior,Pedro I, Y1 - 2019/09/20/ PY - 2019/05/23/received PY - 2019/08/30/accepted PY - 2019/9/22/entrez SP - 13631 EP - 13631 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - One of the most important cellular events in arthropods is the moulting of the cuticle (ecdysis). This process allows them to grow until they reach sexual maturity. Nevertheless, during this stage, the animals are highly exposed to pathogens. Consequently, it can be assumed that arthropods counter with an efficient anti-infective strategy that facilitates their survival during ecdysis. Herein, we characterized a novel antimicrobial peptide called Pinipesin, present in the exuviae extract of the centipede Scolopendra subspinipes subspinipes. The antimicrobial activity of Pinipesin was tested. The haemolytic activity of the peptide was evaluated and its possible mechanism of action was investigated. Identification was carried out by mass spectrometry analysis. Pinipesin displayed potent antimicrobial effects against different microorganisms and showed low haemolytic effects against human erythrocytes at high concentrations. It has a monoisotopic mass of 1213.57 Da, its sequence exhibited high similarity with some cuticular proteins, and it might act intracellularly by interfering with protein synthesis. Our data suggest that Pinipesin might be part of a prophylactic immune response during the ecdysis process of centipedes. Therefore, it is a promising candidate for the development of non-conventional antibiotics that could help fight infectious diseases and represents an exciting discovery for this taxon. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31541146/Antimicrobial_activity_and_mechanism_of_action_of_a_novel_peptide_present_in_the_ecdysis_process_of_centipede_Scolopendra_subspinipes_subspinipes L2 - http://dx.doi.org/10.1038/s41598-019-50061-y DB - PRIME DP - Unbound Medicine ER -