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Feedlot performance and biological responses to coated and non-coated steroidal implants containing trenbolone acetate and estradiol benzoate in finishing beef steers.
J Anim Sci 2019JA

Abstract

Predominately Angus steers (n = 24; initial BW = 435 ± 28.3 kg) were used to evaluate non-coated and coated implants containing equal amounts of trenbolone acetate (TBA; 200 mg) and estradiol benzoate (EB; 28 mg) in finishing steers on sera metabolite responses, gene expression, and immunohistochemical analyses of the Longissimus muscle. Performance data were analyzed as a randomized complete block design and all other data were analyzed as repeated measures for a completely randomized design. Treatments were no implant (NI), non-coated (NC; Synovex-PLUS; Zoetis, Parsippany, NJ) and coated (CI; Synovex-One Feedlot) implant. There were 2 pen replicates per treatment (n = 4 steers/pen). Longissimus muscle (LM) biopsies, blood, and BW were collected before feeding on day (d) 0, 14, 28, 56, 84, 112, and 133, with final BW being captured on d 140. Genes of interest were determined by RT-qPCR using two housekeeping genes. Sera was analyzed for estradiol-17β (E2),17β-trenbolone (TbOH), IGF-I, NEFA, and urea-N (SUN). An α of 0.10 determined significance for performance and sera data; α of 0.05 was used for gene and histology data. No performance differences (P ≥ 0.10) were detected. An implant × d interaction (P ≤ 0.10) for E2, IGF-I, and SUN was detected; implants elevated (P ≤ 0.10) E2, 17β-TbOH, and IGF-I; and decreased SUN across d of the study, meaning sera metabolites are not altered with time on feed. An implant × d interaction was detected for myogenic factor 5 (Myf-5) positive cells and proportions of MHCIIX. In LM, CI had greater (P < 0.10) IGF-I in LM over NI. Coated implant increased (P < 0.05) GPER1 expression, as well as, GPER1 semi quantitative scores over NI and NC. An implant × d interaction (P ≤ 0.05) for estrogen and androgen receptor positive nuclei was detected; implants had increased (P ≤ 0.05) estrogen and androgen receptor positive nuclei compared to NI. Coated implants increase genes associated with muscle tissue growth.

Authors+Show Affiliations

Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX.Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX.Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31541251

Citation

Smith, Z K., et al. "Feedlot Performance and Biological Responses to Coated and Non-coated Steroidal Implants Containing Trenbolone Acetate and Estradiol Benzoate in Finishing Beef Steers." Journal of Animal Science, 2019.
Smith ZK, Kim J, Johnson BJ. Feedlot performance and biological responses to coated and non-coated steroidal implants containing trenbolone acetate and estradiol benzoate in finishing beef steers. J Anim Sci. 2019.
Smith, Z. K., Kim, J., & Johnson, B. J. (2019). Feedlot performance and biological responses to coated and non-coated steroidal implants containing trenbolone acetate and estradiol benzoate in finishing beef steers. Journal of Animal Science, doi:10.1093/jas/skz298.
Smith ZK, Kim J, Johnson BJ. Feedlot Performance and Biological Responses to Coated and Non-coated Steroidal Implants Containing Trenbolone Acetate and Estradiol Benzoate in Finishing Beef Steers. J Anim Sci. 2019 Sep 21; PubMed PMID: 31541251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Feedlot performance and biological responses to coated and non-coated steroidal implants containing trenbolone acetate and estradiol benzoate in finishing beef steers. AU - Smith,Z K, AU - Kim,J, AU - Johnson,B J, Y1 - 2019/09/21/ PY - 2019/02/19/received PY - 2019/9/22/entrez KW - beef KW - estradiol KW - insulin-like growth factor I KW - satellite cell KW - trenbolone JF - Journal of animal science JO - J. Anim. Sci. N2 - Predominately Angus steers (n = 24; initial BW = 435 ± 28.3 kg) were used to evaluate non-coated and coated implants containing equal amounts of trenbolone acetate (TBA; 200 mg) and estradiol benzoate (EB; 28 mg) in finishing steers on sera metabolite responses, gene expression, and immunohistochemical analyses of the Longissimus muscle. Performance data were analyzed as a randomized complete block design and all other data were analyzed as repeated measures for a completely randomized design. Treatments were no implant (NI), non-coated (NC; Synovex-PLUS; Zoetis, Parsippany, NJ) and coated (CI; Synovex-One Feedlot) implant. There were 2 pen replicates per treatment (n = 4 steers/pen). Longissimus muscle (LM) biopsies, blood, and BW were collected before feeding on day (d) 0, 14, 28, 56, 84, 112, and 133, with final BW being captured on d 140. Genes of interest were determined by RT-qPCR using two housekeeping genes. Sera was analyzed for estradiol-17β (E2),17β-trenbolone (TbOH), IGF-I, NEFA, and urea-N (SUN). An α of 0.10 determined significance for performance and sera data; α of 0.05 was used for gene and histology data. No performance differences (P ≥ 0.10) were detected. An implant × d interaction (P ≤ 0.10) for E2, IGF-I, and SUN was detected; implants elevated (P ≤ 0.10) E2, 17β-TbOH, and IGF-I; and decreased SUN across d of the study, meaning sera metabolites are not altered with time on feed. An implant × d interaction was detected for myogenic factor 5 (Myf-5) positive cells and proportions of MHCIIX. In LM, CI had greater (P < 0.10) IGF-I in LM over NI. Coated implant increased (P < 0.05) GPER1 expression, as well as, GPER1 semi quantitative scores over NI and NC. An implant × d interaction (P ≤ 0.05) for estrogen and androgen receptor positive nuclei was detected; implants had increased (P ≤ 0.05) estrogen and androgen receptor positive nuclei compared to NI. Coated implants increase genes associated with muscle tissue growth. SN - 1525-3163 UR - https://www.unboundmedicine.com/medline/citation/31541251/Feedlot_performance_and_biological_responses_to_coated_and_non-coated_steroidal_implants_containing_trenbolone_acetate_and_estradiol_benzoate_in_finishing_beef_steers L2 - https://academic.oup.com/jas/article-lookup/doi/10.1093/jas/skz298 DB - PRIME DP - Unbound Medicine ER -