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Development of an LC-MS/MS method for quantitative analysis of Chlorogenic acid in human plasma and its application to a pharmacokinetic study in Chinese patients with advanced solid tumor.
J Pharm Biomed Anal 2019; 177:112809JP

Abstract

A simple and specific, rapid resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for determination of chlorogenic acid in human plasma using neochlorogenic acid as the internal standard. Plasma samples were precipitated with methanol and separated on a Zorbax C18 column (50 × 2.1 mm, i.d. 1.8 μm) at a flow rate of 0.4 mL/min using a gradient mobile phase of methanol-water containing 0.1% formic acid (v/v). The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring in negative ESI mode. The method was fully validated over the concentration range of 10-2000 ng/mL. The indicators of inter- and intra-day precision (RSD%) were all within 10.7%, and the accuracy (RE%) was ranged from -3.0% to 10.6%. Moreover, we evaluated this bioanalytical method by re-analysis of incurred samples as an additional measure of assay reproducibility. This method was successfully applied to pharmacokinetic study of CGA in Chinese subjects with advanced solid tumor after intramuscular injection administration of Chlorogenic acid for injection (CAFI).

Authors+Show Affiliations

Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), National drug clinical trial center, Peking University Cancer Hospital & Institute, Haidian District, Beijing, PR China.Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gastrointestinal Oncology, Peking University Cancer Hospital & Institute, Haidian District, Beijing, PR China. Electronic address: goodjf@163.com.Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gastrointestinal Oncology, Peking University Cancer Hospital & Institute, Haidian District, Beijing, PR China.Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Clinical Laboratory, Peking University Cancer Hospital & Institute, Haidian District, Beijing, PR China.Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Daycare Center, Peking University Cancer Hospital & Institute, Haidian District, Beijing, PR China.Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gastrointestinal Oncology, Peking University Cancer Hospital & Institute, Haidian District, Beijing, PR China.Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), National drug clinical trial center, Peking University Cancer Hospital & Institute, Haidian District, Beijing, PR China.Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Clinical Laboratory, Peking University Cancer Hospital & Institute, Haidian District, Beijing, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31541942

Citation

Yang, Fen, et al. "Development of an LC-MS/MS Method for Quantitative Analysis of Chlorogenic Acid in Human Plasma and Its Application to a Pharmacokinetic Study in Chinese Patients With Advanced Solid Tumor." Journal of Pharmaceutical and Biomedical Analysis, vol. 177, 2019, p. 112809.
Yang F, Gong JF, Shen L, et al. Development of an LC-MS/MS method for quantitative analysis of Chlorogenic acid in human plasma and its application to a pharmacokinetic study in Chinese patients with advanced solid tumor. J Pharm Biomed Anal. 2019;177:112809.
Yang, F., Gong, J. F., Shen, L., Zhang, C., Kou, F. R., Gao, J., ... Bing Xu, G. (2019). Development of an LC-MS/MS method for quantitative analysis of Chlorogenic acid in human plasma and its application to a pharmacokinetic study in Chinese patients with advanced solid tumor. Journal of Pharmaceutical and Biomedical Analysis, 177, p. 112809. doi:10.1016/j.jpba.2019.112809.
Yang F, et al. Development of an LC-MS/MS Method for Quantitative Analysis of Chlorogenic Acid in Human Plasma and Its Application to a Pharmacokinetic Study in Chinese Patients With Advanced Solid Tumor. J Pharm Biomed Anal. 2019 Aug 14;177:112809. PubMed PMID: 31541942.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of an LC-MS/MS method for quantitative analysis of Chlorogenic acid in human plasma and its application to a pharmacokinetic study in Chinese patients with advanced solid tumor. AU - Yang,Fen, AU - Gong,Ji Fang, AU - Shen,Lin, AU - Zhang,Chen, AU - Kou,Fu Rong, AU - Gao,Jing, AU - Li,Yan, AU - Bing Xu,Guo, Y1 - 2019/08/14/ PY - 2019/06/04/received PY - 2019/07/31/revised PY - 2019/08/07/accepted PY - 2019/9/22/pubmed PY - 2019/9/22/medline PY - 2019/9/22/entrez KW - Chlorogenic acid KW - Chlorogenic acid for injection KW - Human plasma KW - LC–MS/MS KW - Pharmacokinetic study SP - 112809 EP - 112809 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 177 N2 - A simple and specific, rapid resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for determination of chlorogenic acid in human plasma using neochlorogenic acid as the internal standard. Plasma samples were precipitated with methanol and separated on a Zorbax C18 column (50 × 2.1 mm, i.d. 1.8 μm) at a flow rate of 0.4 mL/min using a gradient mobile phase of methanol-water containing 0.1% formic acid (v/v). The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring in negative ESI mode. The method was fully validated over the concentration range of 10-2000 ng/mL. The indicators of inter- and intra-day precision (RSD%) were all within 10.7%, and the accuracy (RE%) was ranged from -3.0% to 10.6%. Moreover, we evaluated this bioanalytical method by re-analysis of incurred samples as an additional measure of assay reproducibility. This method was successfully applied to pharmacokinetic study of CGA in Chinese subjects with advanced solid tumor after intramuscular injection administration of Chlorogenic acid for injection (CAFI). SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/31541942/Development_of_an_LC-MS/MS_method_for_quantitative_analysis_of_Chlorogenic_acid_in_human_plasma_and_its_application_to_a_pharmacokinetic_study_in_Chinese_patients_with_advanced_solid_tumor L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(18)30051-7 DB - PRIME DP - Unbound Medicine ER -