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Sexual reproduction of Zymoseptoria tritici on durum wheat in Tunisia revealed by presence of airborne inoculum, fruiting bodies and high levels of genetic diversity.
Fungal Biol. 2019 10; 123(10):763-772.FB

Abstract

Septoria tritici blotch (STB) caused by the heterothallic ascomycete Zymoseptoria tritici is currently one of the most devastating diseases of wheat worldwide. The extent of sexual reproduction of this pathogen is well documented on bread wheat, but not on durum wheat. The objective of the present study was to quantify the occurrence of Z. tritici sexual reproduction on durum wheat in the Tunisian environment. The assessment was undertaken using a triple approach combining fruiting body assessment, ascospore trapping and population genetic analyses. The results highlighted the formation of pseudothecia on leaves and stubble from the autumn until the end of the growing season. Likewise, qPCR monitoring highlighted a constant release of Z. tritici airborne inoculum during the wheat-growing season, with a peak of production at the end of the season. Genetic investigations using microsatellites revealed high levels of gene and genotypic diversities, an equal distribution of mating types, and a lack of genetic clustering within and between growing seasons. Taken together, these findings indicate that Z. tritici undergoes sexual reproduction on durum wheat in Tunisia at least to the same extent than on bread wheat in Western Europe, and that the dry and warm climate does not affect the mating process of the fungus. Frequent occurrence of sexual reproduction is a valuable knowledge to take into account in STB control strategies on durum wheat.

Authors+Show Affiliations

University of Carthage, National Agronomic Institute of Tunisia, LR14AGR01, Laboratory of Genetics and Cereal Breeding, National Agronomic Institute of Tunisia, Avenue Charles Nicolle 43, 1082 Tunis, Tunisia. Electronic address: marwa.hassine1@gmail.com.ISA, INRA, Univ. Artois, Univ. Lille, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: ali.siah@yncrea.fr.Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Croix du Sud 2 Box L7.05.03, 1348 Louvain-la-Neuve, Belgium; Plant Protection and Ecotoxicology Unit, Walloon Agricultural Research Center, Rue du Bordia 11, 5030 Gembloux, Belgium. Electronic address: p.hellin@cra.wallonie.be.ISA, INRA, Univ. Artois, Univ. Lille, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: thierry.cadalen@univ-lille1.fr.ISA, INRA, Univ. Artois, Univ. Lille, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: patrice.halama@yncrea.fr.ISA, INRA, Univ. Artois, Univ. Lille, Univ. Littoral Côte d'Opale, EA 7394 - ICV - Institut Charles Viollette, F-59000 Lille, France. Electronic address: jean-louis.hilbert@univ-lille.fr.University of Carthage, National Agronomic Institute of Tunisia, LR14AGR01, Laboratory of Genetics and Cereal Breeding, National Agronomic Institute of Tunisia, Avenue Charles Nicolle 43, 1082 Tunis, Tunisia. Electronic address: hamada.walid@iresa.agrinet.tn.National Research Institute of Rural Engineering, Water and Forestry, Rue Hédi EL Karray El Menzah IV 1004 2080 Ariana, Tunisia. Electronic address: moktar.baraket@gmail.com.International Maize and Wheat Improvement Center, Carretera México-Veracruz Km. 45, El Batán, 56237 Texcoco, México. Electronic address: ah.yahyaoui@cgiar.org.Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Croix du Sud 2 Box L7.05.03, 1348 Louvain-la-Neuve, Belgium. Electronic address: anne.legreve@uclouvain.be.Plant Protection and Ecotoxicology Unit, Walloon Agricultural Research Center, Rue du Bordia 11, 5030 Gembloux, Belgium. Electronic address: m.duvivier@cra.wallonie.be.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31542193

Citation

Hassine, M, et al. "Sexual Reproduction of Zymoseptoria Tritici On Durum Wheat in Tunisia Revealed By Presence of Airborne Inoculum, Fruiting Bodies and High Levels of Genetic Diversity." Fungal Biology, vol. 123, no. 10, 2019, pp. 763-772.
Hassine M, Siah A, Hellin P, et al. Sexual reproduction of Zymoseptoria tritici on durum wheat in Tunisia revealed by presence of airborne inoculum, fruiting bodies and high levels of genetic diversity. Fungal Biol. 2019;123(10):763-772.
Hassine, M., Siah, A., Hellin, P., Cadalen, T., Halama, P., Hilbert, J. L., Hamada, W., Baraket, M., Yahyaoui, A., Legrève, A., & Duvivier, M. (2019). Sexual reproduction of Zymoseptoria tritici on durum wheat in Tunisia revealed by presence of airborne inoculum, fruiting bodies and high levels of genetic diversity. Fungal Biology, 123(10), 763-772. https://doi.org/10.1016/j.funbio.2019.06.006
Hassine M, et al. Sexual Reproduction of Zymoseptoria Tritici On Durum Wheat in Tunisia Revealed By Presence of Airborne Inoculum, Fruiting Bodies and High Levels of Genetic Diversity. Fungal Biol. 2019;123(10):763-772. PubMed PMID: 31542193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sexual reproduction of Zymoseptoria tritici on durum wheat in Tunisia revealed by presence of airborne inoculum, fruiting bodies and high levels of genetic diversity. AU - Hassine,M, AU - Siah,A, AU - Hellin,P, AU - Cadalen,T, AU - Halama,P, AU - Hilbert,J-L, AU - Hamada,W, AU - Baraket,M, AU - Yahyaoui,A, AU - Legrève,A, AU - Duvivier,M, Y1 - 2019/06/22/ PY - 2018/10/17/received PY - 2019/04/05/revised PY - 2019/06/19/accepted PY - 2019/9/23/entrez PY - 2019/9/23/pubmed PY - 2020/5/15/medline KW - Ascospores KW - Epidemiology KW - Population genetics KW - Septoria tritici blotch KW - qPCR SP - 763 EP - 772 JF - Fungal biology JO - Fungal Biol VL - 123 IS - 10 N2 - Septoria tritici blotch (STB) caused by the heterothallic ascomycete Zymoseptoria tritici is currently one of the most devastating diseases of wheat worldwide. The extent of sexual reproduction of this pathogen is well documented on bread wheat, but not on durum wheat. The objective of the present study was to quantify the occurrence of Z. tritici sexual reproduction on durum wheat in the Tunisian environment. The assessment was undertaken using a triple approach combining fruiting body assessment, ascospore trapping and population genetic analyses. The results highlighted the formation of pseudothecia on leaves and stubble from the autumn until the end of the growing season. Likewise, qPCR monitoring highlighted a constant release of Z. tritici airborne inoculum during the wheat-growing season, with a peak of production at the end of the season. Genetic investigations using microsatellites revealed high levels of gene and genotypic diversities, an equal distribution of mating types, and a lack of genetic clustering within and between growing seasons. Taken together, these findings indicate that Z. tritici undergoes sexual reproduction on durum wheat in Tunisia at least to the same extent than on bread wheat in Western Europe, and that the dry and warm climate does not affect the mating process of the fungus. Frequent occurrence of sexual reproduction is a valuable knowledge to take into account in STB control strategies on durum wheat. SN - 1878-6146 UR - https://www.unboundmedicine.com/medline/citation/31542193/Sexual_reproduction_of_Zymoseptoria_tritici_on_durum_wheat_in_Tunisia_revealed_by_presence_of_airborne_inoculum_fruiting_bodies_and_high_levels_of_genetic_diversity_ DB - PRIME DP - Unbound Medicine ER -