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Androgens augment pulmonary responses to ozone in mice.
Physiol Rep 2019; 7(18):e14214PR

Abstract

Ozone causes airway hyperresponsiveness, a defining feature of asthma, and is an asthma trigger. In mice, ozone-induced airway hyperresponsiveness is greater in males than in females, suggesting a role for sex hormones in the response to ozone. To examine the role of androgens in these sex differences, we castrated 4-week-old mice. Controls underwent sham surgery. At 8 weeks of age, mice were exposed to ozone (2ppm, 3 h) or room air. Twenty-four hours later, mice were anesthetized and measurements of airway responsiveness to inhaled aerosolized methacholine were made. Mice were then euthanized and bronchoalveolar lavage was performed. Castration attenuated ozone-induced airway hyperresponsiveness and reduced bronchoalveolar lavage cells. In intact males, flutamide, an androgen receptor inhibitor, had similar effects to castration. Bronchoalveolar lavage concentrations of several cytokines were reduced by either castration or flutamide treatment, but only IL-1α was reduced by both castration and flutamide. Furthermore, an anti-IL-1α antibody reduced bronchoalveolar lavage neutrophils in intact males, although it did not alter ozone-induced airway hyperresponsiveness. Our data indicate that androgens augment pulmonary responses to ozone and that IL-1α may contribute to the effects of androgens on ozone-induced cellular inflammation but not airway hyperresponsiveness.

Authors+Show Affiliations

Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, Massachusetts.Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, Massachusetts.Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, Massachusetts.Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, Massachusetts.Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, Massachusetts.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31544355

Citation

Osgood, Ross S., et al. "Androgens Augment Pulmonary Responses to Ozone in Mice." Physiological Reports, vol. 7, no. 18, 2019, pp. e14214.
Osgood RS, Kasahara DI, Tashiro H, et al. Androgens augment pulmonary responses to ozone in mice. Physiol Rep. 2019;7(18):e14214.
Osgood, R. S., Kasahara, D. I., Tashiro, H., Cho, Y., & Shore, S. A. (2019). Androgens augment pulmonary responses to ozone in mice. Physiological Reports, 7(18), pp. e14214. doi:10.14814/phy2.14214.
Osgood RS, et al. Androgens Augment Pulmonary Responses to Ozone in Mice. Physiol Rep. 2019;7(18):e14214. PubMed PMID: 31544355.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Androgens augment pulmonary responses to ozone in mice. AU - Osgood,Ross S, AU - Kasahara,David I, AU - Tashiro,Hiroki, AU - Cho,Youngji, AU - Shore,Stephanie A, PY - 2019/06/06/received PY - 2019/08/05/revised PY - 2019/08/06/accepted PY - 2019/9/24/entrez PY - 2019/9/24/pubmed PY - 2019/9/24/medline KW - Airway hyperresponsiveness KW - IL-1α KW - castration KW - flutamide SP - e14214 EP - e14214 JF - Physiological reports JO - Physiol Rep VL - 7 IS - 18 N2 - Ozone causes airway hyperresponsiveness, a defining feature of asthma, and is an asthma trigger. In mice, ozone-induced airway hyperresponsiveness is greater in males than in females, suggesting a role for sex hormones in the response to ozone. To examine the role of androgens in these sex differences, we castrated 4-week-old mice. Controls underwent sham surgery. At 8 weeks of age, mice were exposed to ozone (2ppm, 3 h) or room air. Twenty-four hours later, mice were anesthetized and measurements of airway responsiveness to inhaled aerosolized methacholine were made. Mice were then euthanized and bronchoalveolar lavage was performed. Castration attenuated ozone-induced airway hyperresponsiveness and reduced bronchoalveolar lavage cells. In intact males, flutamide, an androgen receptor inhibitor, had similar effects to castration. Bronchoalveolar lavage concentrations of several cytokines were reduced by either castration or flutamide treatment, but only IL-1α was reduced by both castration and flutamide. Furthermore, an anti-IL-1α antibody reduced bronchoalveolar lavage neutrophils in intact males, although it did not alter ozone-induced airway hyperresponsiveness. Our data indicate that androgens augment pulmonary responses to ozone and that IL-1α may contribute to the effects of androgens on ozone-induced cellular inflammation but not airway hyperresponsiveness. SN - 2051-817X UR - https://www.unboundmedicine.com/medline/citation/31544355/Androgens_augment_pulmonary_responses_to_ozone_in_mice_ L2 - https://doi.org/10.14814/phy2.14214 DB - PRIME DP - Unbound Medicine ER -