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Expression of Long Non-Coding RNAs by Human Retinal Müller Glial Cells Infected with Clonal and Exotic Virulent Toxoplasma gondii.
Noncoding RNA 2019; 5(4)NR

Abstract

Retinal infection with Toxoplasma gondii-ocular toxoplasmosis-is a common cause of vision impairment worldwide. Pathology combines parasite-induced retinal cell death and reactive intraocular inflammation. Müller glial cells, which represent the supporting cell population of the retina, are relatively susceptible to infection with T. gondii. We investigated expression of long non-coding RNAs (lncRNAs) with immunologic regulatory activity in Müller cells infected with virulent T. gondii strains-GT1 (haplogroup 1, type I) and GPHT (haplogroup 6). We first confirmed expression of 33 lncRNA in primary cell isolates. MIO-M1 human retinal Müller cell monolayers were infected with T. gondii tachyzoites (multiplicity of infection = 5) and harvested at 4, 12, 24, and 36 h post-infection, with infection being tracked by the expression of parasite surface antigen 1 (SAG1). Significant fold-changes were observed for 31 lncRNAs at one or more time intervals. Similar changes between strains were measured for BANCR, CYTOR, FOXD3-AS1, GAS5, GSTT1-AS1, LINC-ROR, LUCAT1, MALAT1, MIR22HG, MIR143HG, PVT1, RMRP, SNHG15, and SOCS2-AS1. Changes differing between strains were measured for APTR, FIRRE, HOTAIR, HOXD-AS1, KCNQ1OT1, LINC00968, LINC01105, lnc-SGK1, MEG3, MHRT, MIAT, MIR17HG, MIR155HG, NEAT1, NeST, NRON, and PACER. Our findings suggest roles for lncRNAs in regulating retinal Müller cell immune responses to T. gondii, and encourage future studies on lncRNA as biomarkers and/or drug targets in ocular toxoplasmosis.

Authors+Show Affiliations

Flinders University College of Medicine & Public Health, Adelaide, SA 5042, Australia. rochet.elise@gmail.com.Flinders University College of Medicine & Public Health, Adelaide, SA 5042, Australia. binoy.appukuttan@flinders.edu.au.Flinders University College of Medicine & Public Health, Adelaide, SA 5042, Australia. yuefang.ma@flinders.edu.au.Flinders University College of Medicine & Public Health, Adelaide, SA 5042, Australia. liam.ashander@flinders.edu.au.Flinders University College of Medicine & Public Health, Adelaide, SA 5042, Australia. justine.smith@flinders.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31547203

Citation

Rochet, Elise, et al. "Expression of Long Non-Coding RNAs By Human Retinal Müller Glial Cells Infected With Clonal and Exotic Virulent Toxoplasma Gondii." Non-coding RNA, vol. 5, no. 4, 2019.
Rochet E, Appukuttan B, Ma Y, et al. Expression of Long Non-Coding RNAs by Human Retinal Müller Glial Cells Infected with Clonal and Exotic Virulent Toxoplasma gondii. Noncoding RNA. 2019;5(4).
Rochet, E., Appukuttan, B., Ma, Y., Ashander, L. M., & Smith, J. R. (2019). Expression of Long Non-Coding RNAs by Human Retinal Müller Glial Cells Infected with Clonal and Exotic Virulent Toxoplasma gondii. Non-coding RNA, 5(4), doi:10.3390/ncrna5040048.
Rochet E, et al. Expression of Long Non-Coding RNAs By Human Retinal Müller Glial Cells Infected With Clonal and Exotic Virulent Toxoplasma Gondii. Noncoding RNA. 2019 Sep 20;5(4) PubMed PMID: 31547203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of Long Non-Coding RNAs by Human Retinal Müller Glial Cells Infected with Clonal and Exotic Virulent Toxoplasma gondii. AU - Rochet,Elise, AU - Appukuttan,Binoy, AU - Ma,Yuefang, AU - Ashander,Liam M, AU - Smith,Justine R, Y1 - 2019/09/20/ PY - 2019/08/15/received PY - 2019/09/16/revised PY - 2019/09/18/accepted PY - 2019/9/25/entrez KW - Müller cells KW - Toxoplasma gondii KW - eye KW - human KW - long non-coding RNA KW - retina KW - toxoplasmosis JF - Non-coding RNA JO - Noncoding RNA VL - 5 IS - 4 N2 - Retinal infection with Toxoplasma gondii-ocular toxoplasmosis-is a common cause of vision impairment worldwide. Pathology combines parasite-induced retinal cell death and reactive intraocular inflammation. Müller glial cells, which represent the supporting cell population of the retina, are relatively susceptible to infection with T. gondii. We investigated expression of long non-coding RNAs (lncRNAs) with immunologic regulatory activity in Müller cells infected with virulent T. gondii strains-GT1 (haplogroup 1, type I) and GPHT (haplogroup 6). We first confirmed expression of 33 lncRNA in primary cell isolates. MIO-M1 human retinal Müller cell monolayers were infected with T. gondii tachyzoites (multiplicity of infection = 5) and harvested at 4, 12, 24, and 36 h post-infection, with infection being tracked by the expression of parasite surface antigen 1 (SAG1). Significant fold-changes were observed for 31 lncRNAs at one or more time intervals. Similar changes between strains were measured for BANCR, CYTOR, FOXD3-AS1, GAS5, GSTT1-AS1, LINC-ROR, LUCAT1, MALAT1, MIR22HG, MIR143HG, PVT1, RMRP, SNHG15, and SOCS2-AS1. Changes differing between strains were measured for APTR, FIRRE, HOTAIR, HOXD-AS1, KCNQ1OT1, LINC00968, LINC01105, lnc-SGK1, MEG3, MHRT, MIAT, MIR17HG, MIR155HG, NEAT1, NeST, NRON, and PACER. Our findings suggest roles for lncRNAs in regulating retinal Müller cell immune responses to T. gondii, and encourage future studies on lncRNA as biomarkers and/or drug targets in ocular toxoplasmosis. SN - 2311-553X UR - https://www.unboundmedicine.com/medline/citation/31547203/Expression_of_Long_Non-Coding_RNAs_by_Human_Retinal_Müller_Glial_Cells_Infected_with_Clonal_and_Exotic_Virulent_Toxoplasma_gondii DB - PRIME DP - Unbound Medicine ER -