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Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium crepidatum.
Biomolecules. 2019 09 12; 9(9)B

Abstract

Dendrobium crepidatum is an epiphytic orchid found in south Asia including Nepal and China. This orchid species is widely used in traditional Chinese medicine (TCM) for the treatment of cancer, diabetes, cataracts, and fever. The objectives of the present research were to assess the antioxidant and cytotoxic properties of its stem's extracts with the identification of bioactive secondary metabolites. The antioxidant and cytotoxic activities were evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, respectively, and compounds were identified using GC-MS (gas chromatography and mass spectrometry). Ethanol and acetone extracts scavenged 94.69 ± 0.10% and 93.41 ± 0.86% of DPPH free radicals, respectively. They showed 50% inhibition of DPPH free radicals (IC50) at concentrations of 73.90 µg/mL and 99.44 µg/mL, which were found to be statistically similar to that of ascorbic acid (control). Chloroform extract inhibited the growth of 81.49 ± 0.43% of HeLa (human cervical carcinoma) cells and hexane extract inhibited the growth of 76.45 ± 4.26% of U251 (human glioblastoma) cells at 800 µg/mL concentration. These extracts showed 50% inhibition of cell growth (IC50) toward both the HeLa and U251 cell lines at their high concentrations, which were found statistically significantly different from that of cisplatin drug (control). The above extracts showed antioxidant and cytotoxic properties, potentially due to the presence of tetracosane, triacontane, stigmasterol, and some phenol derivatives (2-methoxy-4-vinylphenol, 2-methoxy-5-(1-propenyl)-phenol, p-mesyloxyphenol, and 2,6-dimethoxy-4-(2-propenyl)-phenol). This study explores the potential of this orchid in alternative medicine toward the development of drugs from its medicinally active compounds.

Authors+Show Affiliations

Central Department of Botany, Tribhuvan University, Kathmandu 44613, Nepal. mr.paudel@cdbtu.edu.np.Central Department of Botany, Tribhuvan University, Kathmandu 44613, Nepal.Annapurna Research Center, Kathmandu 44600, Nepal.Central Department of Botany, Tribhuvan University, Kathmandu 44613, Nepal.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31547263

Citation

Paudel, Mukti Ram, et al. "Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium Crepidatum." Biomolecules, vol. 9, no. 9, 2019.
Paudel MR, Chand MB, Pant B, et al. Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium crepidatum. Biomolecules. 2019;9(9).
Paudel, M. R., Chand, M. B., Pant, B., & Pant, B. (2019). Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium crepidatum. Biomolecules, 9(9). https://doi.org/10.3390/biom9090478
Paudel MR, et al. Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium Crepidatum. Biomolecules. 2019 09 12;9(9) PubMed PMID: 31547263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium crepidatum. AU - Paudel,Mukti Ram, AU - Chand,Mukesh Babu, AU - Pant,Basant, AU - Pant,Bijaya, Y1 - 2019/09/12/ PY - 2019/08/02/received PY - 2019/08/25/revised PY - 2019/08/28/accepted PY - 2019/9/25/entrez PY - 2019/9/25/pubmed PY - 2020/7/7/medline KW - DPPH KW - GC–MS KW - MTT KW - extract KW - orchid JF - Biomolecules JO - Biomolecules VL - 9 IS - 9 N2 - Dendrobium crepidatum is an epiphytic orchid found in south Asia including Nepal and China. This orchid species is widely used in traditional Chinese medicine (TCM) for the treatment of cancer, diabetes, cataracts, and fever. The objectives of the present research were to assess the antioxidant and cytotoxic properties of its stem's extracts with the identification of bioactive secondary metabolites. The antioxidant and cytotoxic activities were evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, respectively, and compounds were identified using GC-MS (gas chromatography and mass spectrometry). Ethanol and acetone extracts scavenged 94.69 ± 0.10% and 93.41 ± 0.86% of DPPH free radicals, respectively. They showed 50% inhibition of DPPH free radicals (IC50) at concentrations of 73.90 µg/mL and 99.44 µg/mL, which were found to be statistically similar to that of ascorbic acid (control). Chloroform extract inhibited the growth of 81.49 ± 0.43% of HeLa (human cervical carcinoma) cells and hexane extract inhibited the growth of 76.45 ± 4.26% of U251 (human glioblastoma) cells at 800 µg/mL concentration. These extracts showed 50% inhibition of cell growth (IC50) toward both the HeLa and U251 cell lines at their high concentrations, which were found statistically significantly different from that of cisplatin drug (control). The above extracts showed antioxidant and cytotoxic properties, potentially due to the presence of tetracosane, triacontane, stigmasterol, and some phenol derivatives (2-methoxy-4-vinylphenol, 2-methoxy-5-(1-propenyl)-phenol, p-mesyloxyphenol, and 2,6-dimethoxy-4-(2-propenyl)-phenol). This study explores the potential of this orchid in alternative medicine toward the development of drugs from its medicinally active compounds. SN - 2218-273X UR - https://www.unboundmedicine.com/medline/citation/31547263/Assessment_of_Antioxidant_and_Cytotoxic_Activities_of_Extracts_of_Dendrobium_crepidatum_ L2 - https://www.mdpi.com/resolver?pii=biom9090478 DB - PRIME DP - Unbound Medicine ER -