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Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region.
Malar J 2019; 18(1):327MJ

Abstract

BACKGROUND

Different antigens are needed to characterize Plasmodium falciparum infection in terms of seroreactivity and targets for invasion inhibition, in order to guide and identify the proper use of such proteins as tools for the development of serological markers and/or as vaccine candidates.

METHODS

IgG responses in 84 serum samples from individuals with P. falciparum infection [classified as symptomatic (Sym) or asymptomatic (Asym)], or acute Plasmodium vivax infection, from the Peruvian Amazon region, were evaluated by enzyme-linked immunosorbent assays specific for a baculovirus-produced recombinant protein P. falciparum Merozoite Surface Protein 10 (rMSP10) and for non-EGF region selected peptides of PfMSP10 selected by a bioinformatics tool (PfMSP10-1, PfMSP10-2 and PfMSP10-3). Monoclonal antibodies against the selected peptides were evaluated by western blotting, confocal microscopy and inhibition invasion assays.

RESULTS

Seroreactivity analysis of the P. falciparum Sym- and Asym-infected individuals against rMSP10 showed a higher response as compared to the individuals with P. vivax acute infection. IgG responses against peptide PfMSP10-1 were weak. Interestingly high IgG response was found against peptide PfMSP10-2 and the combination of peptides PfMSP10-1 + PfMSP10-2. Monoclonal antibodies were capable of detecting native PfMSP10 on purified schizonts by western blot and confocal microscopy. A low percentage of inhibition of merozoite invasion of erythrocytes in vitro was observed when the monoclonal antibodies were compared with the control antibody against AMA-1 antigen. Further studies are needed to evaluate the role of PfMSP10 in the merozoite invasion.

CONCLUSIONS

The rMSP10 and the PfMSP10-2 peptide synthesized for this study may be useful antigens for evaluation of P. falciparum malaria exposure in Sym and Asym individuals from the Peruvian Amazon region. Moreover, these antigens can be used for further investigation of the role of this protein in other malaria-endemic areas.

Authors+Show Affiliations

Laboratorios de Investigación y Desarrollo, FARVET, Carretera Panamericana Sur No 766 km 198.5, Chincha Alta, Ica, Peru. eduar156@yahoo.es.Laboratorios de Investigación y Desarrollo "Abraham Vaisberg Wolach, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru. Instituto de Medicina Tropical Alexander von Humboldt-Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martín de Porres, Lima, Peru.Laboratorios de Investigación y Desarrollo, FARVET, Carretera Panamericana Sur No 766 km 198.5, Chincha Alta, Ica, Peru.Laboratorios de Investigación y Desarrollo "Abraham Vaisberg Wolach, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.Laboratorios de Investigación y Desarrollo "Abraham Vaisberg Wolach, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.Laboratorios de Investigación y Desarrollo "Abraham Vaisberg Wolach, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.Laboratorios de Investigación y Desarrollo "Abraham Vaisberg Wolach, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.Laboratorios de Investigación y Desarrollo, FARVET, Carretera Panamericana Sur No 766 km 198.5, Chincha Alta, Ica, Peru.Laboratorios de Investigación y Desarrollo "Abraham Vaisberg Wolach, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru. Instituto de Medicina Tropical Alexander von Humboldt-Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martín de Porres, Lima, Peru.Laboratorios de Investigación y Desarrollo "Abraham Vaisberg Wolach, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru. katherine.torres.f@upch.pe. Instituto de Medicina Tropical Alexander von Humboldt-Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martín de Porres, Lima, Peru. katherine.torres.f@upch.pe.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31547821

Citation

Bendezu, Jorge, et al. "Evaluation of Plasmodium Falciparum MSP10 and Its Development as a Serological Tool for the Peruvian Amazon Region." Malaria Journal, vol. 18, no. 1, 2019, p. 327.
Bendezu J, Villasis E, Morales Ruiz S, et al. Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region. Malar J. 2019;18(1):327.
Bendezu, J., Villasis, E., Morales Ruiz, S., Garro, K., Infante, B., Gutierrez-Loli, R., ... Torres, K. (2019). Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region. Malaria Journal, 18(1), p. 327. doi:10.1186/s12936-019-2959-8.
Bendezu J, et al. Evaluation of Plasmodium Falciparum MSP10 and Its Development as a Serological Tool for the Peruvian Amazon Region. Malar J. 2019 Sep 23;18(1):327. PubMed PMID: 31547821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region. AU - Bendezu,Jorge, AU - Villasis,Elizabeth, AU - Morales Ruiz,Sandra, AU - Garro,Katherine, AU - Infante,Berónica, AU - Gutierrez-Loli,Renzo, AU - Rodríguez,Pamela, AU - Fernández-Díaz,Manolo, AU - Gamboa,Dionicia, AU - Torres,Katherine, Y1 - 2019/09/23/ PY - 2019/04/14/received PY - 2019/09/11/accepted PY - 2019/9/25/entrez PY - 2019/9/25/pubmed PY - 2019/9/25/medline KW - Monoclonal antibodies KW - Peptides KW - PfMSP10 KW - Plasmodium falciparum SP - 327 EP - 327 JF - Malaria journal JO - Malar. J. VL - 18 IS - 1 N2 - BACKGROUND: Different antigens are needed to characterize Plasmodium falciparum infection in terms of seroreactivity and targets for invasion inhibition, in order to guide and identify the proper use of such proteins as tools for the development of serological markers and/or as vaccine candidates. METHODS: IgG responses in 84 serum samples from individuals with P. falciparum infection [classified as symptomatic (Sym) or asymptomatic (Asym)], or acute Plasmodium vivax infection, from the Peruvian Amazon region, were evaluated by enzyme-linked immunosorbent assays specific for a baculovirus-produced recombinant protein P. falciparum Merozoite Surface Protein 10 (rMSP10) and for non-EGF region selected peptides of PfMSP10 selected by a bioinformatics tool (PfMSP10-1, PfMSP10-2 and PfMSP10-3). Monoclonal antibodies against the selected peptides were evaluated by western blotting, confocal microscopy and inhibition invasion assays. RESULTS: Seroreactivity analysis of the P. falciparum Sym- and Asym-infected individuals against rMSP10 showed a higher response as compared to the individuals with P. vivax acute infection. IgG responses against peptide PfMSP10-1 were weak. Interestingly high IgG response was found against peptide PfMSP10-2 and the combination of peptides PfMSP10-1 + PfMSP10-2. Monoclonal antibodies were capable of detecting native PfMSP10 on purified schizonts by western blot and confocal microscopy. A low percentage of inhibition of merozoite invasion of erythrocytes in vitro was observed when the monoclonal antibodies were compared with the control antibody against AMA-1 antigen. Further studies are needed to evaluate the role of PfMSP10 in the merozoite invasion. CONCLUSIONS: The rMSP10 and the PfMSP10-2 peptide synthesized for this study may be useful antigens for evaluation of P. falciparum malaria exposure in Sym and Asym individuals from the Peruvian Amazon region. Moreover, these antigens can be used for further investigation of the role of this protein in other malaria-endemic areas. SN - 1475-2875 UR - https://www.unboundmedicine.com/medline/citation/31547821/Evaluation_of_Plasmodium_falciparum_MSP10_and_its_development_as_a_serological_tool_for_the_Peruvian_Amazon_region L2 - https://malariajournal.biomedcentral.com/articles/10.1186/s12936-019-2959-8 DB - PRIME DP - Unbound Medicine ER -