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Role of tissue factor in the procoagulant and antibacterial effects of human adipose-derived mesenchymal stem cells during pneumosepsis in mice.
Stem Cell Res Ther 2019; 10(1):286SC

Abstract

BACKGROUND

Adult mesenchymal stem cells (MSCs) improve the host response during experimental sepsis in animals. MSCs from various sources express a procoagulant activity that has been linked to the expression of tissue factor. This study sought to determine the role of tissue factor associated with adipose-derived MSCs (ASCs) in their procoagulant and antibacterial effects during pneumonia-derived sepsis.

METHODS

Mice were infused intravenously with ASCs or vehicle after infection with the common human pathogen Klebsiella pneumoniae via the airways.

RESULTS

Infusion of freshly cultured or cryopreserved ASCs induced the expression of many genes associated with tissue factor signaling and coagulation activation in the lungs. Freshly cultured and cryopreserved ASCs, as well as ASC lysates, exerted procoagulant activity in vitro as determined by a fibrin generation assay, which was almost completely inhibited by an anti-tissue factor antibody. Infusion of cryopreserved ASCs was associated with a rise in plasma thrombin-antithrombin complexes (indicative of coagulation activation) and formation of multiple thrombi in the lungs 4 h post-infusion. Preincubation of ASCs with anti-tissue factor antibody prior to infusion prevented the rise in plasma thrombin-antithrombin complex concentrations but did not influence thrombus formation in the lungs. ASCs reduced bacterial loads in the lungs and liver at 48 h after infection, which was not influenced by preincubation with anti-tissue factor antibody. At this late time point, microthrombi in the lungs were not detected anymore.

CONCLUSION

These data indicate that ASC-associated tissue factor is responsible for systemic activation of coagulation after infusion of ASCs but not for the formation of microthrombi in the lungs or antibacterial effects.

Authors+Show Affiliations

Center of Experimental & Molecular Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands. d.perlee@amc.uva.nl.Center of Experimental & Molecular Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.Center of Experimental & Molecular Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands. Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.Center of Experimental & Molecular Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.TiGenix SAU, Madrid, Spain.TiGenix SAU, Madrid, Spain.TiGenix NV, Leuven, Belgium.Department of Pathology, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.Center of Experimental & Molecular Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands.TiGenix SAU, Madrid, Spain.Center of Experimental & Molecular Medicine, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands. Division of Infectious Diseases, Amsterdam University Medical Centers, University of Amsterdam, Meibergdreef 9, Room G2-130, 1105AZ, Amsterdam, the Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31547876

Citation

Perlee, Desirée, et al. "Role of Tissue Factor in the Procoagulant and Antibacterial Effects of Human Adipose-derived Mesenchymal Stem Cells During Pneumosepsis in Mice." Stem Cell Research & Therapy, vol. 10, no. 1, 2019, p. 286.
Perlee D, de Vos AF, Scicluna BP, et al. Role of tissue factor in the procoagulant and antibacterial effects of human adipose-derived mesenchymal stem cells during pneumosepsis in mice. Stem Cell Res Ther. 2019;10(1):286.
Perlee, D., de Vos, A. F., Scicluna, B. P., Maag, A., Mancheño, P., de la Rosa, O., ... van der Poll, T. (2019). Role of tissue factor in the procoagulant and antibacterial effects of human adipose-derived mesenchymal stem cells during pneumosepsis in mice. Stem Cell Research & Therapy, 10(1), p. 286. doi:10.1186/s13287-019-1391-x.
Perlee D, et al. Role of Tissue Factor in the Procoagulant and Antibacterial Effects of Human Adipose-derived Mesenchymal Stem Cells During Pneumosepsis in Mice. Stem Cell Res Ther. 2019 Sep 23;10(1):286. PubMed PMID: 31547876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of tissue factor in the procoagulant and antibacterial effects of human adipose-derived mesenchymal stem cells during pneumosepsis in mice. AU - Perlee,Desirée, AU - de Vos,Alex F, AU - Scicluna,Brendon P, AU - Maag,Anja, AU - Mancheño,Pablo, AU - de la Rosa,Olga, AU - Dalemans,Wilfried, AU - Florquin,Sandrine, AU - Van't Veer,Cornelis, AU - Lombardo,Eleuterio, AU - van der Poll,Tom, Y1 - 2019/09/23/ PY - 2019/05/01/received PY - 2019/08/19/accepted PY - 2019/08/03/revised PY - 2019/9/25/entrez PY - 2019/9/25/pubmed PY - 2019/9/25/medline KW - Coagulation KW - Immunomodulation KW - Mesenchymal stem cells KW - Sepsis SP - 286 EP - 286 JF - Stem cell research & therapy JO - Stem Cell Res Ther VL - 10 IS - 1 N2 - BACKGROUND: Adult mesenchymal stem cells (MSCs) improve the host response during experimental sepsis in animals. MSCs from various sources express a procoagulant activity that has been linked to the expression of tissue factor. This study sought to determine the role of tissue factor associated with adipose-derived MSCs (ASCs) in their procoagulant and antibacterial effects during pneumonia-derived sepsis. METHODS: Mice were infused intravenously with ASCs or vehicle after infection with the common human pathogen Klebsiella pneumoniae via the airways. RESULTS: Infusion of freshly cultured or cryopreserved ASCs induced the expression of many genes associated with tissue factor signaling and coagulation activation in the lungs. Freshly cultured and cryopreserved ASCs, as well as ASC lysates, exerted procoagulant activity in vitro as determined by a fibrin generation assay, which was almost completely inhibited by an anti-tissue factor antibody. Infusion of cryopreserved ASCs was associated with a rise in plasma thrombin-antithrombin complexes (indicative of coagulation activation) and formation of multiple thrombi in the lungs 4 h post-infusion. Preincubation of ASCs with anti-tissue factor antibody prior to infusion prevented the rise in plasma thrombin-antithrombin complex concentrations but did not influence thrombus formation in the lungs. ASCs reduced bacterial loads in the lungs and liver at 48 h after infection, which was not influenced by preincubation with anti-tissue factor antibody. At this late time point, microthrombi in the lungs were not detected anymore. CONCLUSION: These data indicate that ASC-associated tissue factor is responsible for systemic activation of coagulation after infusion of ASCs but not for the formation of microthrombi in the lungs or antibacterial effects. SN - 1757-6512 UR - https://www.unboundmedicine.com/medline/citation/31547876/Role_of_tissue_factor_in_the_procoagulant_and_antibacterial_effects_of_human_adipose-derived_mesenchymal_stem_cells_during_pneumosepsis_in_mice L2 - https://stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1391-x DB - PRIME DP - Unbound Medicine ER -