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Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera.
Infect Immun. 2019 12; 87(12)II

Abstract

Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components. Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators. The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities. Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species. MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups. The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition. LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species. Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species. These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS.

Authors+Show Affiliations

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.Department of Microbial Pathogenesis, University of Maryland, Baltimore, Maryland, USA.Division of Pulmonary and Critical Care Medicine, Harborview Medical Center, University of Washington, Seattle, Washington, USA. International Respiratory and Severe Illness Center, University of Washington, Seattle, Washington, USA.Department of Microbial Pathogenesis, University of Maryland, Baltimore, Maryland, USA.Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand narisara@tropmedres.ac. Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31548320

Citation

Sengyee, Sineenart, et al. "Lipopolysaccharides From Different Burkholderia Species With Different Lipid a Structures Induce Toll-Like Receptor 4 Activation and React With Melioidosis Patient Sera." Infection and Immunity, vol. 87, no. 12, 2019.
Sengyee S, Yoon SH, West TE, et al. Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera. Infect Immun. 2019;87(12).
Sengyee, S., Yoon, S. H., West, T. E., Ernst, R. K., & Chantratita, N. (2019). Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera. Infection and Immunity, 87(12). https://doi.org/10.1128/IAI.00692-19
Sengyee S, et al. Lipopolysaccharides From Different Burkholderia Species With Different Lipid a Structures Induce Toll-Like Receptor 4 Activation and React With Melioidosis Patient Sera. Infect Immun. 2019;87(12) PubMed PMID: 31548320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Sera. AU - Sengyee,Sineenart, AU - Yoon,Sung Hwan, AU - West,T Eoin, AU - Ernst,Robert K, AU - Chantratita,Narisara, Y1 - 2019/11/18/ PY - 2019/08/31/received PY - 2019/09/13/accepted PY - 2019/9/25/pubmed PY - 2020/1/22/medline PY - 2019/9/25/entrez KW - Burkholderia KW - LPS structure KW - O-polysaccharide KW - TLR4 KW - antibody KW - bacterial infection KW - diagnostics KW - innate immunity KW - lipopolysaccharide KW - melioidosis KW - serology JF - Infection and immunity JO - Infect Immun VL - 87 IS - 12 N2 - Lipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components. Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators. The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities. Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species. MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups. The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition. LPSs of all species induced TLR4-dependent NF-κB responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species. Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species. These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS. SN - 1098-5522 UR - https://www.unboundmedicine.com/medline/citation/31548320/Lipopolysaccharides_from_Different_Burkholderia_Species_with_Different_Lipid_A_Structures_Induce_Toll_Like_Receptor_4_Activation_and_React_with_Melioidosis_Patient_Sera_ L2 - http://iai.asm.org/lookup/pmidlookup?view=long&pmid=31548320 DB - PRIME DP - Unbound Medicine ER -