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Castor patatin-like phospholipase A IIIβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds.
Plant Mol Biol. 2019 Dec; 101(6):521-536.PM

Abstract

KEY MESSAGE

Castor patatin-like phospholipase A IIIβ facilitates the exclusion of hydroxy fatty acids from phosphatidylcholine in developing transgenic Arabidopsis seeds. Hydroxy fatty acids (HFAs) are industrial useful, but their major natural source castor contains toxic components. Although expressing a castor OLEATE 12-HYDROXYLASE in Arabidopsis thaliana leads to the synthesis of HFAs in seeds, a high proportion of the HFAs are retained in phosphatidylcholine (PC). Thus, the liberation of HFA from PC seems to be critical for obtaining HFA-enriched seed oils. Plant phospholipase A (PLA) catalyzes the hydrolysis of PC to release fatty acyl chains that can be subsequently channeled into triacylglycerol (TAG) synthesis or other metabolic pathways. To further our knowledge regarding the function of PLAs from HFA-producing plant species, two class III patatin-like PLA cDNAs (pPLAIIIβ or pPLAIIIδ) from castor or Physaria fendleri were overexpressed in a transgenic line of A. thaliana producing C18-HFA, respectively. Only the overexpression of RcpPLAIIIβ resulted in a significant reduction in seed HFA content with concomitant changes in fatty acid composition. Reductions in HFA content occurred in both PC and TAG indicating that HFAs released from PC were not incorporated into TAG. These results suggest that RcpPLAIIIβ may catalyze the removal of HFAs from PC in the developing seeds synthesizing these unusual fatty acids.

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Authors+Show Affiliations

Lin Y
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
Chen G
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada. gc24@ualberta.ca.
Mietkiewska E
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada. Okanagan Specialty Fruits Inc. (OSF), 410 Downey Road, Saskatoon, SK, S7N 4N1, Canada.
Song Z
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
Caldo KMP
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
Singer SD
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada. Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada.
Dyer J
USDA-ARS, Arid-Land Agricultural Research Center, 21881 North Cardon Lane, Maricopa, AZ, 85138, USA.
Smith M
Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, S7N 0X2, Canada.
McKeon T
USDA-ARS, Western Regional Research Center, 800 Buchanan St, Albany, CA, 94710, USA.
Weselake RJ
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada. randall.weselake@ualberta.ca.

MeSH

ArabidopsisArabidopsis ProteinsFatty AcidsGene Expression Regulation, PlantPhosphatidylcholinesPhospholipasesPlants, Genetically Modified

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31549344

Citation

Lin, Yingyu, et al. "Castor Patatin-like Phospholipase a IIIβ Facilitates Removal of Hydroxy Fatty Acids From Phosphatidylcholine in Transgenic Arabidopsis Seeds." Plant Molecular Biology, vol. 101, no. 6, 2019, pp. 521-536.
Lin Y, Chen G, Mietkiewska E, et al. Castor patatin-like phospholipase A IIIβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds. Plant Mol Biol. 2019;101(6):521-536.
Lin, Y., Chen, G., Mietkiewska, E., Song, Z., Caldo, K. M. P., Singer, S. D., Dyer, J., Smith, M., McKeon, T., & Weselake, R. J. (2019). Castor patatin-like phospholipase A IIIβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds. Plant Molecular Biology, 101(6), 521-536. https://doi.org/10.1007/s11103-019-00915-w
Lin Y, et al. Castor Patatin-like Phospholipase a IIIβ Facilitates Removal of Hydroxy Fatty Acids From Phosphatidylcholine in Transgenic Arabidopsis Seeds. Plant Mol Biol. 2019;101(6):521-536. PubMed PMID: 31549344.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Castor patatin-like phospholipase A IIIβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds. AU - Lin,Yingyu, AU - Chen,Guanqun, AU - Mietkiewska,Elzbieta, AU - Song,Ziliang, AU - Caldo,Kristian Mark P, AU - Singer,Stacy D, AU - Dyer,John, AU - Smith,Mark, AU - McKeon,Thomas, AU - Weselake,Randall J, Y1 - 2019/09/23/ PY - 2019/04/08/received PY - 2019/09/04/accepted PY - 2019/9/25/pubmed PY - 2019/12/18/medline PY - 2019/9/25/entrez KW - Castor KW - Hydroxy fatty acid KW - Phosphatidylcholine KW - Phospholipase A KW - Physaria fendleri KW - Ricinus communis SP - 521 EP - 536 JF - Plant molecular biology JO - Plant Mol Biol VL - 101 IS - 6 N2 - KEY MESSAGE: Castor patatin-like phospholipase A IIIβ facilitates the exclusion of hydroxy fatty acids from phosphatidylcholine in developing transgenic Arabidopsis seeds. Hydroxy fatty acids (HFAs) are industrial useful, but their major natural source castor contains toxic components. Although expressing a castor OLEATE 12-HYDROXYLASE in Arabidopsis thaliana leads to the synthesis of HFAs in seeds, a high proportion of the HFAs are retained in phosphatidylcholine (PC). Thus, the liberation of HFA from PC seems to be critical for obtaining HFA-enriched seed oils. Plant phospholipase A (PLA) catalyzes the hydrolysis of PC to release fatty acyl chains that can be subsequently channeled into triacylglycerol (TAG) synthesis or other metabolic pathways. To further our knowledge regarding the function of PLAs from HFA-producing plant species, two class III patatin-like PLA cDNAs (pPLAIIIβ or pPLAIIIδ) from castor or Physaria fendleri were overexpressed in a transgenic line of A. thaliana producing C18-HFA, respectively. Only the overexpression of RcpPLAIIIβ resulted in a significant reduction in seed HFA content with concomitant changes in fatty acid composition. Reductions in HFA content occurred in both PC and TAG indicating that HFAs released from PC were not incorporated into TAG. These results suggest that RcpPLAIIIβ may catalyze the removal of HFAs from PC in the developing seeds synthesizing these unusual fatty acids. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/31549344/Castor_patatin_like_phospholipase_A_IIIβ_facilitates_removal_of_hydroxy_fatty_acids_from_phosphatidylcholine_in_transgenic_Arabidopsis_seeds_ L2 - https://doi.org/10.1007/s11103-019-00915-w DB - PRIME DP - Unbound Medicine ER -