Tags

Type your tag names separated by a space and hit enter

Analysis of the effect of methyl 2-acetamide-3-methylquinoxaline-7-carboxylate 1,4-di-N-oxide on the relative expression of the trypanothione reductase gene in Trypanosoma cruzi epimastigotes.
Pak J Pharm Sci. 2019 May; 32(3 Special):1447-1452.PJ

Abstract

In recent decades, some quinoxaline 1,4-di-N-oxide derivatives have been shown to have better trypanocidal activity than the reference drugs; however, their mechanism of action is not yet clear, although it is suggested that they mainly produce reactive oxygen species that cause oxidative stress and parasite death. Trypanosoma cruzi relies on the enzyme trypanothione reductase, among others, to defend itself against oxidative stress. With the aim of contributing to the elucidation of the mechanism of action of quinoxaline 1,4-di-N-oxide derivatives on Trypanosoma cruzi, this study was carried out to evaluate the effect of methyl 2-amide-3-methylquinoxaline-7-carboxylate 1,4-di-N-oxide (compound M-8) on the expression of the trypanothione reductase gene in an in vitro model on Trypanosoma cruzi epimastigotes of the CL-Brener strain. The results show that compound M-8 does not cause a significant effect on the trypanothione reductase gene, suggesting a mechanism of action not related to oxidative stress.

Authors+Show Affiliations

Laboratorio de Biotecnologia Farmaceutica, Centro de Biotecnología Genomica, Instituto Politecnico Nacional, Reynosa, Mexico.Laboratorio de Biotecnologia Farmaceutica, Centro de Biotecnología Genomica, Instituto Politecnico Nacional, Reynosa, Mexico.Laboratorio de Biotecnologia Farmaceutica, Centro de Biotecnología Genomica, Instituto Politecnico Nacional, Reynosa, Mexico.Departamento de Parasitologia, Escuela Nacional de Ciencias Biologicas Instituto Politecnico Nacional, Ciudad de México, Mexico.Laboratorio de Biotecnologia Farmaceutica, Centro de Biotecnología Genomica, Instituto Politecnico Nacional, Reynosa, Mexico.Laboratorio de Biotecnologia Farmaceutica, Centro de Biotecnología Genomica, Instituto Politecnico Nacional, Reynosa, Mexico.Laboratorio de Biotecnologia Farmaceutica, Centro de Biotecnología Genomica, Instituto Politecnico Nacional, Reynosa, Mexico.Laboratorio de Biotecnologia Farmaceutica, Centro de Biotecnología Genomica, Instituto Politecnico Nacional, Reynosa, Mexico.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31551230

Citation

Vazquez-Jimenez, Lenci K K., et al. "Analysis of the Effect of Methyl 2-acetamide-3-methylquinoxaline-7-carboxylate 1,4-di-N-oxide On the Relative Expression of the Trypanothione Reductase Gene in Trypanosoma Cruzi Epimastigotes." Pakistan Journal of Pharmaceutical Sciences, vol. 32, no. 3 Special, 2019, pp. 1447-1452.
Vazquez-Jimenez LKK, Hernandez-Posada MI, Paz-Gonzalez AD, et al. Analysis of the effect of methyl 2-acetamide-3-methylquinoxaline-7-carboxylate 1,4-di-N-oxide on the relative expression of the trypanothione reductase gene in Trypanosoma cruzi epimastigotes. Pak J Pharm Sci. 2019;32(3 Special):1447-1452.
Vazquez-Jimenez, L. K. K., Hernandez-Posada, M. I., Paz-Gonzalez, A. D., Nogueda-Torres, B., Martinez-Vazquez, A. V., Herrera-Mayorga, V., Bocanegra-Garcia, V., & Rivera, G. R. (2019). Analysis of the effect of methyl 2-acetamide-3-methylquinoxaline-7-carboxylate 1,4-di-N-oxide on the relative expression of the trypanothione reductase gene in Trypanosoma cruzi epimastigotes. Pakistan Journal of Pharmaceutical Sciences, 32(3 Special), 1447-1452.
Vazquez-Jimenez LKK, et al. Analysis of the Effect of Methyl 2-acetamide-3-methylquinoxaline-7-carboxylate 1,4-di-N-oxide On the Relative Expression of the Trypanothione Reductase Gene in Trypanosoma Cruzi Epimastigotes. Pak J Pharm Sci. 2019;32(3 Special):1447-1452. PubMed PMID: 31551230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the effect of methyl 2-acetamide-3-methylquinoxaline-7-carboxylate 1,4-di-N-oxide on the relative expression of the trypanothione reductase gene in Trypanosoma cruzi epimastigotes. AU - Vazquez-Jimenez,Lenci K K, AU - Hernandez-Posada,Maria I, AU - Paz-Gonzalez,Alma D, AU - Nogueda-Torres,Benjamin, AU - Martinez-Vazquez,Ana V, AU - Herrera-Mayorga,Veronica, AU - Bocanegra-Garcia,Virgilio, AU - Rivera,Gildardo Rivera, PY - 2019/9/26/entrez PY - 2019/9/26/pubmed PY - 2020/2/13/medline SP - 1447 EP - 1452 JF - Pakistan journal of pharmaceutical sciences JO - Pak J Pharm Sci VL - 32 IS - 3 Special N2 - In recent decades, some quinoxaline 1,4-di-N-oxide derivatives have been shown to have better trypanocidal activity than the reference drugs; however, their mechanism of action is not yet clear, although it is suggested that they mainly produce reactive oxygen species that cause oxidative stress and parasite death. Trypanosoma cruzi relies on the enzyme trypanothione reductase, among others, to defend itself against oxidative stress. With the aim of contributing to the elucidation of the mechanism of action of quinoxaline 1,4-di-N-oxide derivatives on Trypanosoma cruzi, this study was carried out to evaluate the effect of methyl 2-amide-3-methylquinoxaline-7-carboxylate 1,4-di-N-oxide (compound M-8) on the expression of the trypanothione reductase gene in an in vitro model on Trypanosoma cruzi epimastigotes of the CL-Brener strain. The results show that compound M-8 does not cause a significant effect on the trypanothione reductase gene, suggesting a mechanism of action not related to oxidative stress. SN - 1011-601X UR - https://www.unboundmedicine.com/medline/citation/31551230/Analysis_of_the_effect_of_methyl_2_acetamide_3_methylquinoxaline_7_carboxylate_14_di_N_oxide_on_the_relative_expression_of_the_trypanothione_reductase_gene_in_Trypanosoma_cruzi_epimastigotes_ DB - PRIME DP - Unbound Medicine ER -