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Effects of Huangkui Capsule on the Expression of SPARC in the Kidney Tissue of a Rat Model with Diabetic Nephropathy.
Curr Gene Ther. 2019; 19(4):211-215.CG

Abstract

OBJECTIVE

The objective of the research is to investigate the effects of Huangkui capsule on the expression of SPARC in the kidney tissues of diabetic nephropathy.

METHODS

SD rats were divided into three groups: normal control group, untreated DN group and HKC-treated DN group. The therapeutic effects and underlying molecular mechanism of HKC on DN rats induced by streptozotocin were evaluated by the levels of serum creatinine, blood urea nitrogen, 24-hour urinary protein and the expression of SPARC. Pathological changes in kidney tissues were observed through hematoxylin-eosin (HE) staining. Moreover, western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the variation of SPARC.

RESULTS

This study was performed to investigate the effects of HKC on DN in SD rats model and its molecular mechanism. Our results showed that the rats treated with HKC had an improved general state and reduced creatinine, blood urea nitrogen and 24-hour urinary protein levels. The deterioration of renal function was delayed due to treatment with HKC. HE staining was utilized to observe that HKC can improve histopathological findings in the kidney tissues of DN rats, including kidney fibrosis. Results of western blot and qRT-PCR showed that HKC can inhibit the expressions of SPARC in the rat model of DN.

CONCLUSION

The present findings demonstrated that HKC inhibited SPARC level and had significant therapeutic effects on DN.

Authors+Show Affiliations

Department of Science and Education, Heilongjiang Provincial Hospital, Harbin, China.School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.Department of Science and Education, Heilongjiang Provincial Hospital, Harbin, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31553295

Citation

Yang, Xiaoyao, et al. "Effects of Huangkui Capsule On the Expression of SPARC in the Kidney Tissue of a Rat Model With Diabetic Nephropathy." Current Gene Therapy, vol. 19, no. 4, 2019, pp. 211-215.
Yang X, Luo M, Jiang Q, et al. Effects of Huangkui Capsule on the Expression of SPARC in the Kidney Tissue of a Rat Model with Diabetic Nephropathy. Curr Gene Ther. 2019;19(4):211-215.
Yang, X., Luo, M., Jiang, Q., & Wang, Y. (2019). Effects of Huangkui Capsule on the Expression of SPARC in the Kidney Tissue of a Rat Model with Diabetic Nephropathy. Current Gene Therapy, 19(4), 211-215. https://doi.org/10.2174/1566523219666190925112249
Yang X, et al. Effects of Huangkui Capsule On the Expression of SPARC in the Kidney Tissue of a Rat Model With Diabetic Nephropathy. Curr Gene Ther. 2019;19(4):211-215. PubMed PMID: 31553295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Huangkui Capsule on the Expression of SPARC in the Kidney Tissue of a Rat Model with Diabetic Nephropathy. AU - Yang,Xiaoyao, AU - Luo,Meng, AU - Jiang,Qinghua, AU - Wang,Yiwei, PY - 2019/04/11/received PY - 2019/06/03/revised PY - 2019/06/12/accepted PY - 2019/9/26/pubmed PY - 2020/8/19/medline PY - 2019/9/26/entrez KW - Huangkui capsule KW - SPARC KW - TCMs KW - diabetes KW - diabetic nephropathy KW - renal function. SP - 211 EP - 215 JF - Current gene therapy JO - Curr Gene Ther VL - 19 IS - 4 N2 - OBJECTIVE: The objective of the research is to investigate the effects of Huangkui capsule on the expression of SPARC in the kidney tissues of diabetic nephropathy. METHODS: SD rats were divided into three groups: normal control group, untreated DN group and HKC-treated DN group. The therapeutic effects and underlying molecular mechanism of HKC on DN rats induced by streptozotocin were evaluated by the levels of serum creatinine, blood urea nitrogen, 24-hour urinary protein and the expression of SPARC. Pathological changes in kidney tissues were observed through hematoxylin-eosin (HE) staining. Moreover, western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were applied to detect the variation of SPARC. RESULTS: This study was performed to investigate the effects of HKC on DN in SD rats model and its molecular mechanism. Our results showed that the rats treated with HKC had an improved general state and reduced creatinine, blood urea nitrogen and 24-hour urinary protein levels. The deterioration of renal function was delayed due to treatment with HKC. HE staining was utilized to observe that HKC can improve histopathological findings in the kidney tissues of DN rats, including kidney fibrosis. Results of western blot and qRT-PCR showed that HKC can inhibit the expressions of SPARC in the rat model of DN. CONCLUSION: The present findings demonstrated that HKC inhibited SPARC level and had significant therapeutic effects on DN. SN - 1875-5631 UR - https://www.unboundmedicine.com/medline/citation/31553295/Effects_of_Huangkui_Capsule_on_the_Expression_of_SPARC_in_the_Kidney_Tissue_of_a_Rat_Model_with_Diabetic_Nephropathy_ L2 - http://www.eurekaselect.com/175096/article DB - PRIME DP - Unbound Medicine ER -