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Multivariate effects of Chinese keemun black tea grades (Camellia sinensis var. sinensis) on the phenolic composition, antioxidant, antihemolytic and cytotoxic/cytoprotection activities.
Food Res Int. 2019 11; 125:108516.FR

Abstract

The main objectives of the study were to compare the phenolic composition, chemical and biological antioxidant activities, and cytotoxicity towards IMR90, HCT8, and A549 cell lines of eight grades of Chinese keemun black tea (Camellia sinensis var. sinensis) using a statistical approach. No cytotoxic effects were observed on IMR90 normal cells. Our results all together show that the chemical antioxidant capacity of high-grade black teas measured by DPPH, FRAP, and total reducing capacity assays was correspondingly higher than the mean values of low-grade teas and these antioxidant assays were not associated with cytotoxicity towards cancerous cell lines (HCT8 and A549). High grades of Chinese keemun black teas contained higher contents of total phenolics, flavonoids and ortho-diphenols than lower grades and theaflavin-3,3'-di-gallate could only be detected in high black tea grades (T1 and T2). Intermediate-high keemun black tea grades - C1, C3, T1, and T2 - which also had the highest mean values of TPC, flavonoids, o-diphenols, theaflavin-3-gallate, theaflavin-3'-gallate, Fe2+ chelating ability, and chemical antioxidant activity, presented the highest inhibition of Wistar rat's brain oxidation. No clear differentiation and trend were observed between erythrocyte protection and Chinese black tea grades as results clearly showed that intermediate black tea grades (C3 and C4) protected more the human erythrocytes against mechanical stress. Our study shows that although higher Chinese keemun black tea grades (T1 and T2) presented the highest TPC, flavonoids, and chemical antioxidant activity, these in vitro chemical assays were not translated into higher biological activity.

Authors+Show Affiliations

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 230036 Hefei, PR China. Electronic address: zhli2091@sina.com.Graduation School of Food Science and Technology, State University of Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Brazil.Department of Chemistry, State University of Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Brazil.Department of Chemistry, State University of Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Brazil.Department of Biological Sciences, Division of Nutrition, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 714, 37130-000 Alfenas, Brazil.Department of Biological Sciences, Division of Nutrition, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 714, 37130-000 Alfenas, Brazil.State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 230036 Hefei, PR China.Graduation School of Food Science and Technology, State University of Ponta Grossa, Av. Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, Brazil; Innovative Food System, Production Systems Unit, Natural Resources Institute Finland (LUKE), Open Innovation House, FI-02150 Espoo, Finland. Electronic address: dgranato@uepg.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31554085

Citation

Zhang, Liang, et al. "Multivariate Effects of Chinese Keemun Black Tea Grades (Camellia Sinensis Var. Sinensis) On the Phenolic Composition, Antioxidant, Antihemolytic and Cytotoxic/cytoprotection Activities." Food Research International (Ottawa, Ont.), vol. 125, 2019, p. 108516.
Zhang L, Santos JS, Cruz TM, et al. Multivariate effects of Chinese keemun black tea grades (Camellia sinensis var. sinensis) on the phenolic composition, antioxidant, antihemolytic and cytotoxic/cytoprotection activities. Food Res Int. 2019;125:108516.
Zhang, L., Santos, J. S., Cruz, T. M., Marques, M. B., do Carmo, M. A. V., Azevedo, L., Wang, Y., & Granato, D. (2019). Multivariate effects of Chinese keemun black tea grades (Camellia sinensis var. sinensis) on the phenolic composition, antioxidant, antihemolytic and cytotoxic/cytoprotection activities. Food Research International (Ottawa, Ont.), 125, 108516. https://doi.org/10.1016/j.foodres.2019.108516
Zhang L, et al. Multivariate Effects of Chinese Keemun Black Tea Grades (Camellia Sinensis Var. Sinensis) On the Phenolic Composition, Antioxidant, Antihemolytic and Cytotoxic/cytoprotection Activities. Food Res Int. 2019;125:108516. PubMed PMID: 31554085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multivariate effects of Chinese keemun black tea grades (Camellia sinensis var. sinensis) on the phenolic composition, antioxidant, antihemolytic and cytotoxic/cytoprotection activities. AU - Zhang,Liang, AU - Santos,Jânio Sousa, AU - Cruz,Thiago Mendanha, AU - Marques,Mariza Boscacci, AU - do Carmo,Mariana Araújo Vieira, AU - Azevedo,Luciana, AU - Wang,Yijun, AU - Granato,Daniel, Y1 - 2019/06/24/ PY - 2019/04/02/received PY - 2019/06/19/revised PY - 2019/06/21/accepted PY - 2019/9/27/entrez PY - 2019/9/27/pubmed PY - 2019/9/27/medline KW - Antihemolytic effect KW - Antioxidants KW - Antiproliferative agents KW - Catechins KW - Reactive oxygen species SP - 108516 EP - 108516 JF - Food research international (Ottawa, Ont.) JO - Food Res Int VL - 125 N2 - The main objectives of the study were to compare the phenolic composition, chemical and biological antioxidant activities, and cytotoxicity towards IMR90, HCT8, and A549 cell lines of eight grades of Chinese keemun black tea (Camellia sinensis var. sinensis) using a statistical approach. No cytotoxic effects were observed on IMR90 normal cells. Our results all together show that the chemical antioxidant capacity of high-grade black teas measured by DPPH, FRAP, and total reducing capacity assays was correspondingly higher than the mean values of low-grade teas and these antioxidant assays were not associated with cytotoxicity towards cancerous cell lines (HCT8 and A549). High grades of Chinese keemun black teas contained higher contents of total phenolics, flavonoids and ortho-diphenols than lower grades and theaflavin-3,3'-di-gallate could only be detected in high black tea grades (T1 and T2). Intermediate-high keemun black tea grades - C1, C3, T1, and T2 - which also had the highest mean values of TPC, flavonoids, o-diphenols, theaflavin-3-gallate, theaflavin-3'-gallate, Fe2+ chelating ability, and chemical antioxidant activity, presented the highest inhibition of Wistar rat's brain oxidation. No clear differentiation and trend were observed between erythrocyte protection and Chinese black tea grades as results clearly showed that intermediate black tea grades (C3 and C4) protected more the human erythrocytes against mechanical stress. Our study shows that although higher Chinese keemun black tea grades (T1 and T2) presented the highest TPC, flavonoids, and chemical antioxidant activity, these in vitro chemical assays were not translated into higher biological activity. SN - 1873-7145 UR - https://www.unboundmedicine.com/medline/citation/31554085/Multivariate_effects_of_Chinese_keemun_black_tea_grades__Camellia_sinensis_var__sinensis__on_the_phenolic_composition_antioxidant_antihemolytic_and_cytotoxic/cytoprotection_activities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0963-9969(19)30387-4 DB - PRIME DP - Unbound Medicine ER -