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Novel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus coral snake venoms: Phylogenetic relationships and characterization of Clarkitoxin-I-Mdum.
Toxicon. 2019 Dec; 170:85-93.T

Abstract

Micrurus mipartitus and M. dumerilii are the most medically important coral snakes in Colombia. Proteomic characterization of their venoms has previously shown that proteins of the three-finger toxin (3FTx) family are abundant components, especially in M. mipartitus (61%) and to a lesser extent in M. dumerilii (28%). In order to increase knowledge on these toxins, in this work a major 3FTx of M. dumerilii venom (8% of the venom proteins), named Clarkitoxin-I-Mdum, was isolated and characterized. Its amino acid sequence comprises 66 residues, with an isotope-averaged molecular mass of 7537 ± 2 Da and a theoretical pI of 9.36, presenting the conserved pattern of eight cysteines that classifies it as a short-chain (type I) 3FTx. Clarkitoxin-I-Mdum was not lethal to mice by intravenous or intracerebroventricular route and was not cytolytic to myogenic cells in vitro. On the other hand, five coding sequences for 3FTxs were obtained from the venom gland of M. mipartitus. These novel toxin sequences were named Mm3FTx-01 to Mm3FTx-05, all of them also presenting the eight conserved cysteines of short-chain 3FTxs. Phylogenetic analysis revealed high variability of 3FTxs from Micrurus, and ELISA using antibodies raised to the major 3FTxs from M. mipartitus and M. dumerilii confirmed their immunochemical divergence. These results highlight the relevance of performing further studies aiming at a deeper understanding of the functional and antigenic relationships among specific Micrurus toxins, with important implications for the production of antivenoms.

Authors+Show Affiliations

Programa de Ofidismo y Escorpionismo, Universidad de Antioquia, Calle 62 #52-59, Medellín, Colombia.Centro de Investigación en Recursos Naturales y Sustentabilidad (CIRENYS), Universidad Bernardo O'Higgins, Santiago de Chile, Chile. Electronic address: monica.saldarriaga@ubo.cl.Programa de Ofidismo y Escorpionismo, Universidad de Antioquia, Calle 62 #52-59, Medellín, Colombia.Departamento de Microbiología y Parasitología Facultad de Medicina, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Programa de Ofidismo y Escorpionismo, Universidad de Antioquia, Calle 62 #52-59, Medellín, Colombia; Escuela de Microbiología, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31557485

Citation

Rey-Suárez, Paola, et al. "Novel Three-finger Toxins From Micrurus Dumerilii and Micrurus Mipartitus Coral Snake Venoms: Phylogenetic Relationships and Characterization of Clarkitoxin-I-Mdum." Toxicon : Official Journal of the International Society On Toxinology, vol. 170, 2019, pp. 85-93.
Rey-Suárez P, Saldarriaga-Córdoba M, Torres U, et al. Novel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus coral snake venoms: Phylogenetic relationships and characterization of Clarkitoxin-I-Mdum. Toxicon. 2019;170:85-93.
Rey-Suárez, P., Saldarriaga-Córdoba, M., Torres, U., Marin-Villa, M., Lomonte, B., & Núñez, V. (2019). Novel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus coral snake venoms: Phylogenetic relationships and characterization of Clarkitoxin-I-Mdum. Toxicon : Official Journal of the International Society On Toxinology, 170, 85-93. https://doi.org/10.1016/j.toxicon.2019.09.017
Rey-Suárez P, et al. Novel Three-finger Toxins From Micrurus Dumerilii and Micrurus Mipartitus Coral Snake Venoms: Phylogenetic Relationships and Characterization of Clarkitoxin-I-Mdum. Toxicon. 2019;170:85-93. PubMed PMID: 31557485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus coral snake venoms: Phylogenetic relationships and characterization of Clarkitoxin-I-Mdum. AU - Rey-Suárez,Paola, AU - Saldarriaga-Córdoba,Mónica, AU - Torres,Uday, AU - Marin-Villa,Marcel, AU - Lomonte,Bruno, AU - Núñez,Vitelbina, Y1 - 2019/09/23/ PY - 2019/07/08/received PY - 2019/09/13/revised PY - 2019/09/16/accepted PY - 2019/9/27/pubmed PY - 2020/4/9/medline PY - 2019/9/27/entrez KW - Coral snake KW - Micrurus dumerilii KW - Micrurus mipartitus KW - Snake venom KW - Three-finger toxins KW - α-neurotoxin SP - 85 EP - 93 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 170 N2 - Micrurus mipartitus and M. dumerilii are the most medically important coral snakes in Colombia. Proteomic characterization of their venoms has previously shown that proteins of the three-finger toxin (3FTx) family are abundant components, especially in M. mipartitus (61%) and to a lesser extent in M. dumerilii (28%). In order to increase knowledge on these toxins, in this work a major 3FTx of M. dumerilii venom (8% of the venom proteins), named Clarkitoxin-I-Mdum, was isolated and characterized. Its amino acid sequence comprises 66 residues, with an isotope-averaged molecular mass of 7537 ± 2 Da and a theoretical pI of 9.36, presenting the conserved pattern of eight cysteines that classifies it as a short-chain (type I) 3FTx. Clarkitoxin-I-Mdum was not lethal to mice by intravenous or intracerebroventricular route and was not cytolytic to myogenic cells in vitro. On the other hand, five coding sequences for 3FTxs were obtained from the venom gland of M. mipartitus. These novel toxin sequences were named Mm3FTx-01 to Mm3FTx-05, all of them also presenting the eight conserved cysteines of short-chain 3FTxs. Phylogenetic analysis revealed high variability of 3FTxs from Micrurus, and ELISA using antibodies raised to the major 3FTxs from M. mipartitus and M. dumerilii confirmed their immunochemical divergence. These results highlight the relevance of performing further studies aiming at a deeper understanding of the functional and antigenic relationships among specific Micrurus toxins, with important implications for the production of antivenoms. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/31557485/Novel_three_finger_toxins_from_Micrurus_dumerilii_and_Micrurus_mipartitus_coral_snake_venoms:_Phylogenetic_relationships_and_characterization_of_Clarkitoxin_I_Mdum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(19)30459-3 DB - PRIME DP - Unbound Medicine ER -