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Medium optimization and kinetic modeling for the production of Aspergillus niger inulinase.
Bioprocess Biosyst Eng. 2020 Feb; 43(2):217-232.BB

Abstract

The goals of this study were to optimize the medium formulation for enhanced production of Aspergillus niger inulinase using Plackett-Burman Design (PBD) and to model the fermentation in optimal medium formulation. Results indicated that (NH4)2SO4 (negative effect), yeast extract and peptone (positive effect) were determined as significant factors affecting the inulinase production. Different media including Medium A (non-enriched), Medium B (contains both negative and positive factors) and Medium C (contains only positive factors) were formed and inulinase fermentations were performed. Findings showed that the best nutritional formulation was Medium C, which yielded to be 1011.02 U/mL, 834.28 U/mL, 1.22, 4383.44 U/mg, 4186 U/mg, 158.49 U/mL/day, 128.60 U/mL/day and 94.54% of PInulinase, SInulinase, I/S ratio, SInulinase, SSucrase, QInulinase, QSucrase and SUY, respectively. Additionally, fungal growth, enzyme or protein production and substrate consumption were modeled using the logistic model, Luedeking-Piret model, and modified Luedeking-Piret model, respectively, and found that enzyme or protein production was non-growth associated. Besides, maintenance value (Z) was lower than γ value, indicating that A. niger mainly utilizes the sugars for enzyme production and fungal growth. Consequently, optimum medium composition was successfully determined by PBD and also the kinetic models fitted the experimental data very well with high regression coefficient.

Authors+Show Affiliations

Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey.Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey.Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey.Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey.Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey.Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey. iturhan@akdeniz.edu.tr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31559507

Citation

Germec, Mustafa, et al. "Medium Optimization and Kinetic Modeling for the Production of Aspergillus Niger Inulinase." Bioprocess and Biosystems Engineering, vol. 43, no. 2, 2020, pp. 217-232.
Germec M, Gürler HN, Ozcan A, et al. Medium optimization and kinetic modeling for the production of Aspergillus niger inulinase. Bioprocess Biosyst Eng. 2020;43(2):217-232.
Germec, M., Gürler, H. N., Ozcan, A., Erkan, S. B., Karahalil, E., & Turhan, I. (2020). Medium optimization and kinetic modeling for the production of Aspergillus niger inulinase. Bioprocess and Biosystems Engineering, 43(2), 217-232. https://doi.org/10.1007/s00449-019-02219-1
Germec M, et al. Medium Optimization and Kinetic Modeling for the Production of Aspergillus Niger Inulinase. Bioprocess Biosyst Eng. 2020;43(2):217-232. PubMed PMID: 31559507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Medium optimization and kinetic modeling for the production of Aspergillus niger inulinase. AU - Germec,Mustafa, AU - Gürler,Hilal Nur, AU - Ozcan,Ali, AU - Erkan,Selime Benemir, AU - Karahalil,Ercan, AU - Turhan,Irfan, Y1 - 2019/09/26/ PY - 2019/04/25/received PY - 2019/09/16/accepted PY - 2019/9/29/pubmed PY - 2019/9/29/medline PY - 2019/9/28/entrez KW - Aspergillus niger KW - Inulinase KW - Kinetic modeling KW - Medium optimization KW - Sucrase KW - Sugar beet molasses SP - 217 EP - 232 JF - Bioprocess and biosystems engineering JO - Bioprocess Biosyst Eng VL - 43 IS - 2 N2 - The goals of this study were to optimize the medium formulation for enhanced production of Aspergillus niger inulinase using Plackett-Burman Design (PBD) and to model the fermentation in optimal medium formulation. Results indicated that (NH4)2SO4 (negative effect), yeast extract and peptone (positive effect) were determined as significant factors affecting the inulinase production. Different media including Medium A (non-enriched), Medium B (contains both negative and positive factors) and Medium C (contains only positive factors) were formed and inulinase fermentations were performed. Findings showed that the best nutritional formulation was Medium C, which yielded to be 1011.02 U/mL, 834.28 U/mL, 1.22, 4383.44 U/mg, 4186 U/mg, 158.49 U/mL/day, 128.60 U/mL/day and 94.54% of PInulinase, SInulinase, I/S ratio, SInulinase, SSucrase, QInulinase, QSucrase and SUY, respectively. Additionally, fungal growth, enzyme or protein production and substrate consumption were modeled using the logistic model, Luedeking-Piret model, and modified Luedeking-Piret model, respectively, and found that enzyme or protein production was non-growth associated. Besides, maintenance value (Z) was lower than γ value, indicating that A. niger mainly utilizes the sugars for enzyme production and fungal growth. Consequently, optimum medium composition was successfully determined by PBD and also the kinetic models fitted the experimental data very well with high regression coefficient. SN - 1615-7605 UR - https://www.unboundmedicine.com/medline/citation/31559507/Medium_optimization_and_kinetic_modeling_for_the_production_of_Aspergillus_niger_inulinase_ L2 - https://dx.doi.org/10.1007/s00449-019-02219-1 DB - PRIME DP - Unbound Medicine ER -
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