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Barley isochorismate synthase mutant is phylloquinone-deficient, but has normal basal salicylic acid level.
Plant Signal Behav 2019; 14(11):1671122PS

Abstract

Salicylic acid (SA) is an important signaling hormone in plant immunity. It can be synthesized by either the phenylpropanoid pathway or the isochorismate pathway, but mutant studies of this have been scarce in other species than Arabidopsis. Here we identified a mutation that introduced a stop-codon early in the barley gene for isochorismate synthase (ICS). We found that homozygous ics plants wilted if not sprayed with 1,4-dihydroxy-2-naphthoic acid, a precursor of phylloquinone, also synthesized via the isochorismate pathway. Interestingly, ics had unchanged SA, suggesting that the basal level of SA is synthesized via the phenylpropanoid pathway. Previous studies have failed seeing increased SA levels in barley after attack by the powdery mildew fungus, Blumeria graminis f.sp. hordei (Bgh), and indeed, we saw no changes in the interaction of ics with this fungus. Overall, we hope this mutant will be useful for other studies of SA in barley.

Authors+Show Affiliations

Department of Plant and Environmental Sciences, Copenhagen Plant Science Centre, University of Copenhagen , Copenhagen , Denmark.Department of Plant and Environmental Sciences, Copenhagen Plant Science Centre, University of Copenhagen , Copenhagen , Denmark.Institute of Biology, Neuchâtel Platform Analytical Chemistry, University of Neuchâtel , Neuchâtel , Switzerland.Department of Plant and Environmental Sciences, Copenhagen Plant Science Centre, University of Copenhagen , Copenhagen , Denmark.Department of Plant and Environmental Sciences, Copenhagen Plant Science Centre, University of Copenhagen , Copenhagen , Denmark.Department of Plant and Environmental Sciences, Copenhagen Plant Science Centre, University of Copenhagen , Copenhagen , Denmark.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31559895

Citation

Qin, Yuan, et al. "Barley Isochorismate Synthase Mutant Is Phylloquinone-deficient, but Has Normal Basal Salicylic Acid Level." Plant Signaling & Behavior, vol. 14, no. 11, 2019, p. 1671122.
Qin Y, Torp AM, Glauser G, et al. Barley isochorismate synthase mutant is phylloquinone-deficient, but has normal basal salicylic acid level. Plant Signal Behav. 2019;14(11):1671122.
Qin, Y., Torp, A. M., Glauser, G., Pedersen, C., Rasmussen, S. K., & Thordal-Christensen, H. (2019). Barley isochorismate synthase mutant is phylloquinone-deficient, but has normal basal salicylic acid level. Plant Signaling & Behavior, 14(11), p. 1671122. doi:10.1080/15592324.2019.1671122.
Qin Y, et al. Barley Isochorismate Synthase Mutant Is Phylloquinone-deficient, but Has Normal Basal Salicylic Acid Level. Plant Signal Behav. 2019;14(11):1671122. PubMed PMID: 31559895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Barley isochorismate synthase mutant is phylloquinone-deficient, but has normal basal salicylic acid level. AU - Qin,Yuan, AU - Torp,Anna Maria, AU - Glauser,Gaëtan, AU - Pedersen,Carsten, AU - Rasmussen,Søren K, AU - Thordal-Christensen,Hans, Y1 - 2019/09/27/ PY - 2020/09/27/pmc-release PY - 2019/9/29/pubmed PY - 2019/9/29/medline PY - 2019/9/28/entrez KW - Salicylic acid KW - barley KW - isochorismate synthase KW - phylloquinone SP - 1671122 EP - 1671122 JF - Plant signaling & behavior JO - Plant Signal Behav VL - 14 IS - 11 N2 - Salicylic acid (SA) is an important signaling hormone in plant immunity. It can be synthesized by either the phenylpropanoid pathway or the isochorismate pathway, but mutant studies of this have been scarce in other species than Arabidopsis. Here we identified a mutation that introduced a stop-codon early in the barley gene for isochorismate synthase (ICS). We found that homozygous ics plants wilted if not sprayed with 1,4-dihydroxy-2-naphthoic acid, a precursor of phylloquinone, also synthesized via the isochorismate pathway. Interestingly, ics had unchanged SA, suggesting that the basal level of SA is synthesized via the phenylpropanoid pathway. Previous studies have failed seeing increased SA levels in barley after attack by the powdery mildew fungus, Blumeria graminis f.sp. hordei (Bgh), and indeed, we saw no changes in the interaction of ics with this fungus. Overall, we hope this mutant will be useful for other studies of SA in barley. SN - 1559-2324 UR - https://www.unboundmedicine.com/medline/citation/31559895/Barley_isochorismate_synthase_mutant_is_phylloquinone-deficient,_but_has_normal_basal_salicylic_acid_level L2 - http://www.tandfonline.com/doi/full/10.1080/15592324.2019.1671122 DB - PRIME DP - Unbound Medicine ER -