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Determination of vitamin A in blood serum based on solid-phase extraction using cetyltrimethyl ammonium bromide-modified attapulgite.
J Sep Sci. 2019 Dec; 42(23):3521-3527.JS

Abstract

Cetyltrimethyl ammonium bromide-modified attapulgite was prepared and utilized as a novel sorbent in a simple solid-phase extraction method for the determination of vitamin A in blood serum. Several factors affecting extraction efficiency were systematically optimized, including the sampling solvent and its volume, as well as the elution solvent and its volume. Under the optimal solid-phase extraction conditions, the adsorption capacity of vitamin A was as high as 28 mg/g according to the Langmuir isotherm model. Based on the developed solid-phase extraction method, the level of vitamin A in 200 µL blood serum sample could be accurately determined by high-performance liquid chromatography. The recoveries of vitamin A spiked in 10% v/v methanol aqueous solutions were in the range of 86.9-92.8%, with the relative standard deviations not more than 8.1%. The method was applied to the determination of vitamin A in serum samples from 20 pregnant women. Compared with the previously reported solid-phase extraction methods for determination of vitamin A in serum, our developed cetyltrimethyl ammonium bromide-modified attapulgite-based solid-phase extraction method used lower serum volume, omitted extra steps (i.e. evaporation and re-dissolution), and eliminated internal standard. The results were promising for it to be used in routine monitoring during pregnancy.

Authors+Show Affiliations

The Affiliated Hospital of Medical School of Ningbo University, Ningbo, P. R. China.Fenghua Institute, Ningbo University of Technology, Ningbo, P. R. China. School of Materials and Chemical Engineering, Ningbo University of Technology, Ningbo, P. R. China.Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.School of Materials and Chemical Engineering, Ningbo University of Technology, Ningbo, P. R. China.School of Materials and Chemical Engineering, Ningbo University of Technology, Ningbo, P. R. China.Ningbo Entry-Exit Inspection and Quarantine Bureau Technical Center, Ningbo, P. R. China.The Affiliated Hospital of Medical School of Ningbo University, Ningbo, P. R. China.Fenghua Institute, Ningbo University of Technology, Ningbo, P. R. China. School of Materials and Chemical Engineering, Ningbo University of Technology, Ningbo, P. R. China.School of Materials and Chemical Engineering, Ningbo University of Technology, Ningbo, P. R. China.Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

31560415

Citation

Xuan, Rongrong, et al. "Determination of Vitamin a in Blood Serum Based On Solid-phase Extraction Using Cetyltrimethyl Ammonium Bromide-modified Attapulgite." Journal of Separation Science, vol. 42, no. 23, 2019, pp. 3521-3527.
Xuan R, Wang T, Hou C, et al. Determination of vitamin A in blood serum based on solid-phase extraction using cetyltrimethyl ammonium bromide-modified attapulgite. J Sep Sci. 2019;42(23):3521-3527.
Xuan, R., Wang, T., Hou, C., Li, X., Li, Y., Chen, Y., Gao, Y., Qiu, D., Xiao, X., Zhang, L., & Zhang, Y. (2019). Determination of vitamin A in blood serum based on solid-phase extraction using cetyltrimethyl ammonium bromide-modified attapulgite. Journal of Separation Science, 42(23), 3521-3527. https://doi.org/10.1002/jssc.201900778
Xuan R, et al. Determination of Vitamin a in Blood Serum Based On Solid-phase Extraction Using Cetyltrimethyl Ammonium Bromide-modified Attapulgite. J Sep Sci. 2019;42(23):3521-3527. PubMed PMID: 31560415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of vitamin A in blood serum based on solid-phase extraction using cetyltrimethyl ammonium bromide-modified attapulgite. AU - Xuan,Rongrong, AU - Wang,Tingting, AU - Hou,Chunyan, AU - Li,Xie, AU - Li,Ying, AU - Chen,Yihui, AU - Gao,Yajie, AU - Qiu,Dan, AU - Xiao,Xunwen, AU - Zhang,Lihua, AU - Zhang,Yukui, Y1 - 2019/10/10/ PY - 2019/08/01/received PY - 2019/09/20/revised PY - 2019/09/21/accepted PY - 2019/9/29/pubmed PY - 2020/4/11/medline PY - 2019/9/28/entrez KW - attapulgite KW - cetyltrimethyl ammonium bromide KW - serum KW - solid-phase extraction KW - vitamin A SP - 3521 EP - 3527 JF - Journal of separation science JO - J Sep Sci VL - 42 IS - 23 N2 - Cetyltrimethyl ammonium bromide-modified attapulgite was prepared and utilized as a novel sorbent in a simple solid-phase extraction method for the determination of vitamin A in blood serum. Several factors affecting extraction efficiency were systematically optimized, including the sampling solvent and its volume, as well as the elution solvent and its volume. Under the optimal solid-phase extraction conditions, the adsorption capacity of vitamin A was as high as 28 mg/g according to the Langmuir isotherm model. Based on the developed solid-phase extraction method, the level of vitamin A in 200 µL blood serum sample could be accurately determined by high-performance liquid chromatography. The recoveries of vitamin A spiked in 10% v/v methanol aqueous solutions were in the range of 86.9-92.8%, with the relative standard deviations not more than 8.1%. The method was applied to the determination of vitamin A in serum samples from 20 pregnant women. Compared with the previously reported solid-phase extraction methods for determination of vitamin A in serum, our developed cetyltrimethyl ammonium bromide-modified attapulgite-based solid-phase extraction method used lower serum volume, omitted extra steps (i.e. evaporation and re-dissolution), and eliminated internal standard. The results were promising for it to be used in routine monitoring during pregnancy. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/31560415/Determination_of_vitamin_A_in_blood_serum_based_on_solid-phase_extraction_using_cetyltrimethyl_ammonium_bromide-modified_attapulgite L2 - https://doi.org/10.1002/jssc.201900778 DB - PRIME DP - Unbound Medicine ER -