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Small-scale wastewater treatment plants as a source of the dissemination of antibiotic resistance genes in the aquatic environment.
J Hazard Mater. 2020 01 05; 381:121221.JH

Abstract

Wastewater treatment plants (WWTPs) are significant source of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), which can spread further in the environment by reaching rivers together with effluents discharged from WWTPs. In this study untreated and treated wastewater (UWW, TWW), upstream and downstream river water (URW, DRW) were collected from 4 WWTPs, in the winter and autumn seasons. The occurrence of ARB resistant to beta-lactams and tetracyclines as well as the presence of antibiotics from these classes were analysed in water and wastewater samples. Additionally, the amounts of 12 ARGs, 2 genes of mobile genetic elements (MGEs), gene uidA identifying E. coli and 16S rRNA were also determined. Resistance to beta-lactams prevailed among ARB in water and wastewater samples (constituting 82-88% of total counts of bacteria). The dominant genes in water and wastewater samples were blaTEM, tetA, sul1. The gene blaOXA demonstrated high variability of its concentration in samples collected in both seasons. Despite the high per cent reduction of ARB and ARGs concentration observed during the wastewater treatment processes, their large quantities are still transmitted into the environment. The research focuses on WWTPs' role in the dissemination of ARGs and MGEs in the aquatic environment.

Authors+Show Affiliations

Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720, Olsztyn, Poland.Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720, Olsztyn, Poland. Electronic address: ewa.korzeniewska@uwm.edu.pl.Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720, Olsztyn, Poland.Environmental Biotechnology Department, Faculty of Energy and Environmental Engineering, Silesian University of Technology, Akademicka 2 Str., 44-100, Gliwice, Poland; The Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 Str., 44-100, Gliwice, Poland.Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100, Gliwice, Poland; The Biotechnology Centre, Silesian University of Technology, Krzywoustego 8 Str., 44-100, Gliwice, Poland.Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720, Olsztyn, Poland.Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720, Olsztyn, Poland.Chair of Plant Raw Materials Chemistry and Processing, Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Pl. Cieszyński 1, 10-957, Olsztyn, Poland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31561123

Citation

Osińska, Adriana, et al. "Small-scale Wastewater Treatment Plants as a Source of the Dissemination of Antibiotic Resistance Genes in the Aquatic Environment." Journal of Hazardous Materials, vol. 381, 2020, p. 121221.
Osińska A, Korzeniewska E, Harnisz M, et al. Small-scale wastewater treatment plants as a source of the dissemination of antibiotic resistance genes in the aquatic environment. J Hazard Mater. 2020;381:121221.
Osińska, A., Korzeniewska, E., Harnisz, M., Felis, E., Bajkacz, S., Jachimowicz, P., Niestępski, S., & Konopka, I. (2020). Small-scale wastewater treatment plants as a source of the dissemination of antibiotic resistance genes in the aquatic environment. Journal of Hazardous Materials, 381, 121221. https://doi.org/10.1016/j.jhazmat.2019.121221
Osińska A, et al. Small-scale Wastewater Treatment Plants as a Source of the Dissemination of Antibiotic Resistance Genes in the Aquatic Environment. J Hazard Mater. 2020 01 5;381:121221. PubMed PMID: 31561123.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Small-scale wastewater treatment plants as a source of the dissemination of antibiotic resistance genes in the aquatic environment. AU - Osińska,Adriana, AU - Korzeniewska,Ewa, AU - Harnisz,Monika, AU - Felis,Ewa, AU - Bajkacz,Sylwia, AU - Jachimowicz,Piotr, AU - Niestępski,Sebastian, AU - Konopka,Iwona, Y1 - 2019/09/13/ PY - 2019/08/06/received PY - 2019/09/11/revised PY - 2019/09/11/accepted PY - 2019/9/29/pubmed PY - 2019/9/29/medline PY - 2019/9/28/entrez KW - ARB KW - ARGs KW - Antibiotics KW - MGEs KW - River water KW - Wastewater SP - 121221 EP - 121221 JF - Journal of hazardous materials JO - J. Hazard. Mater. VL - 381 N2 - Wastewater treatment plants (WWTPs) are significant source of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), which can spread further in the environment by reaching rivers together with effluents discharged from WWTPs. In this study untreated and treated wastewater (UWW, TWW), upstream and downstream river water (URW, DRW) were collected from 4 WWTPs, in the winter and autumn seasons. The occurrence of ARB resistant to beta-lactams and tetracyclines as well as the presence of antibiotics from these classes were analysed in water and wastewater samples. Additionally, the amounts of 12 ARGs, 2 genes of mobile genetic elements (MGEs), gene uidA identifying E. coli and 16S rRNA were also determined. Resistance to beta-lactams prevailed among ARB in water and wastewater samples (constituting 82-88% of total counts of bacteria). The dominant genes in water and wastewater samples were blaTEM, tetA, sul1. The gene blaOXA demonstrated high variability of its concentration in samples collected in both seasons. Despite the high per cent reduction of ARB and ARGs concentration observed during the wastewater treatment processes, their large quantities are still transmitted into the environment. The research focuses on WWTPs' role in the dissemination of ARGs and MGEs in the aquatic environment. SN - 1873-3336 UR - https://www.unboundmedicine.com/medline/citation/31561123/Small_scale_wastewater_treatment_plants_as_a_source_of_the_dissemination_of_antibiotic_resistance_genes_in_the_aquatic_environment_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3894(19)31175-6 DB - PRIME DP - Unbound Medicine ER -
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