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Large scale production of lacto-N-biose I, a building block of type I human milk oligosaccharides, using sugar phosphorylases.
Biosci Biotechnol Biochem. 2020 Jan; 84(1):17-24.BB

Abstract

Human milk oligosaccharides (HMOs) have drawn attention for their contribution to the explosive bifidobacterial growth in the intestines of neonates. We found that bifidobacteria can efficiently metabolize lacto-N-biose I (LNB), the major building blocks of HMOs, and we have developed a method to synthesize LNB by applying this system. We produced LNB on a kilogram scale by the method. This proved that, among the enterobacteria, only bifidobacteria can assimilate LNB, and provided the data that supported the explosive growth of bifidobacteria in neonates. Furthermore, we were also able to reveal the structure of LNB crystal and the low stability for heating at neutral pH, which has not been clarified so far. In this paper, using bifidobacteria and LNB as examples, I describe the research on oligosaccharide synthesis that was conducted by utilizing a sugar metabolism.Abbreviations: LNB: lacto-N-biose I; GNB: galacto-N-biose; HMOs: human milk oligosaccharides; GLNBP: GNB/LNB phosphorylase; NahK: N-acetylhexosamine 1-kinase; GalT: UDP-glucose-hexose-1-phosphate uridylyltransferase; GalE: UDP-glucose 4-epimerase; SP: sucrose phosphorylase.

Authors+Show Affiliations

Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31566084

Citation

Nishimoto, Mamoru. "Large Scale Production of lacto-N-biose I, a Building Block of Type I Human Milk Oligosaccharides, Using Sugar Phosphorylases." Bioscience, Biotechnology, and Biochemistry, vol. 84, no. 1, 2020, pp. 17-24.
Nishimoto M. Large scale production of lacto-N-biose I, a building block of type I human milk oligosaccharides, using sugar phosphorylases. Biosci Biotechnol Biochem. 2020;84(1):17-24.
Nishimoto, M. (2020). Large scale production of lacto-N-biose I, a building block of type I human milk oligosaccharides, using sugar phosphorylases. Bioscience, Biotechnology, and Biochemistry, 84(1), 17-24. https://doi.org/10.1080/09168451.2019.1670047
Nishimoto M. Large Scale Production of lacto-N-biose I, a Building Block of Type I Human Milk Oligosaccharides, Using Sugar Phosphorylases. Biosci Biotechnol Biochem. 2020;84(1):17-24. PubMed PMID: 31566084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Large scale production of lacto-N-biose I, a building block of type I human milk oligosaccharides, using sugar phosphorylases. A1 - Nishimoto,Mamoru, Y1 - 2019/09/28/ PY - 2019/10/1/pubmed PY - 2020/1/14/medline PY - 2019/10/1/entrez KW - Lacto-N-biose I KW - growth factor for bifidobacteria KW - sugar phosphorylase SP - 17 EP - 24 JF - Bioscience, biotechnology, and biochemistry JO - Biosci. Biotechnol. Biochem. VL - 84 IS - 1 N2 - Human milk oligosaccharides (HMOs) have drawn attention for their contribution to the explosive bifidobacterial growth in the intestines of neonates. We found that bifidobacteria can efficiently metabolize lacto-N-biose I (LNB), the major building blocks of HMOs, and we have developed a method to synthesize LNB by applying this system. We produced LNB on a kilogram scale by the method. This proved that, among the enterobacteria, only bifidobacteria can assimilate LNB, and provided the data that supported the explosive growth of bifidobacteria in neonates. Furthermore, we were also able to reveal the structure of LNB crystal and the low stability for heating at neutral pH, which has not been clarified so far. In this paper, using bifidobacteria and LNB as examples, I describe the research on oligosaccharide synthesis that was conducted by utilizing a sugar metabolism.Abbreviations: LNB: lacto-N-biose I; GNB: galacto-N-biose; HMOs: human milk oligosaccharides; GLNBP: GNB/LNB phosphorylase; NahK: N-acetylhexosamine 1-kinase; GalT: UDP-glucose-hexose-1-phosphate uridylyltransferase; GalE: UDP-glucose 4-epimerase; SP: sucrose phosphorylase. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/31566084/Large_scale_production_of_lacto-N-biose_I,_a_building_block_of_type_I_human_milk_oligosaccharides,_using_sugar_phosphorylases L2 - http://www.tandfonline.com/doi/full/10.1080/09168451.2019.1670047 DB - PRIME DP - Unbound Medicine ER -