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Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor.

Abstract

An accurate, rapid, and cost-effective biosensor for the quantification of disease biomarkers is vital for the development of early-diagnostic point-of-care systems. The recent discovery of the trans-cleavage property of CRISPR type V effectors makes CRISPR a potential high-accuracy bio-recognition tool. Herein, a CRISPR-Cas12a (cpf1) based electrochemical biosensor (E-CRISPR) is reported, which is more cost-effective and portable than optical-transduction-based biosensors. Through optimizing the in vitro trans-cleavage activity of Cas12a, E-CRIPSR was used to detect viral nucleic acids, including human papillomavirus 16 (HPV-16) and parvovirus B19 (PB-19), with a picomolar sensitivity. An aptamer-based E-CRISPR cascade was further designed for the detection of transforming growth factor β1 (TGF-β1) protein in clinical samples. As demonstrated, E-CRISPR could enable the development of portable, accurate, and cost-effective point-of-care diagnostic systems.

Authors+Show Affiliations

Department of Chemical and Biomolecular Engineering, Electronics Design Center, Case Western Reserve University, Cleveland, OH, 44106, USA.Department of Biology, Skeletal Research Center &, Center for Multimodal Evaluation of Engineered Cartilage, Case Western Reserve University, Cleveland, OH, 44106, USA.Department of Genetics and Genome Sciences, School of Medicine, Case Western Reserve University, Cleveland, OH, 44106, USA.Department of Biology, Skeletal Research Center &, Center for Multimodal Evaluation of Engineered Cartilage, Case Western Reserve University, Cleveland, OH, 44106, USA.Department of Genetics and Genome Sciences, School of Medicine, Case Western Reserve University, Cleveland, OH, 44106, USA.Department of Biology, Skeletal Research Center &, Center for Multimodal Evaluation of Engineered Cartilage, Case Western Reserve University, Cleveland, OH, 44106, USA.Department of Chemical and Biomolecular Engineering, Electronics Design Center, Case Western Reserve University, Cleveland, OH, 44106, USA.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

31568601

Citation

Dai, Yifan, et al. "Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor." Angewandte Chemie (International Ed. in English), 2019.
Dai Y, Somoza RA, Wang L, et al. Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor. Angew Chem Int Ed Engl. 2019.
Dai, Y., Somoza, R. A., Wang, L., Welter, J. F., Li, Y., Caplan, A. I., & Liu, C. C. (2019). Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor. Angewandte Chemie (International Ed. in English), doi:10.1002/anie.201910772.
Dai Y, et al. Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor. Angew Chem Int Ed Engl. 2019 Sep 30; PubMed PMID: 31568601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor. AU - Dai,Yifan, AU - Somoza,Rodrigo A, AU - Wang,Liu, AU - Welter,Jean F, AU - Li,Yan, AU - Caplan,Arnold I, AU - Liu,Chung Chiun, Y1 - 2019/09/30/ PY - 2019/08/22/received PY - 2019/09/23/revised PY - 2019/10/1/pubmed PY - 2019/10/1/medline PY - 2019/10/1/entrez KW - CRISPR Cas12a (cpf1) KW - bioanalytical chemistry KW - biosensor KW - electrochemistry KW - trans-acting cleavage JF - Angewandte Chemie (International ed. in English) JO - Angew. Chem. Int. Ed. Engl. N2 - An accurate, rapid, and cost-effective biosensor for the quantification of disease biomarkers is vital for the development of early-diagnostic point-of-care systems. The recent discovery of the trans-cleavage property of CRISPR type V effectors makes CRISPR a potential high-accuracy bio-recognition tool. Herein, a CRISPR-Cas12a (cpf1) based electrochemical biosensor (E-CRISPR) is reported, which is more cost-effective and portable than optical-transduction-based biosensors. Through optimizing the in vitro trans-cleavage activity of Cas12a, E-CRIPSR was used to detect viral nucleic acids, including human papillomavirus 16 (HPV-16) and parvovirus B19 (PB-19), with a picomolar sensitivity. An aptamer-based E-CRISPR cascade was further designed for the detection of transforming growth factor β1 (TGF-β1) protein in clinical samples. As demonstrated, E-CRISPR could enable the development of portable, accurate, and cost-effective point-of-care diagnostic systems. SN - 1521-3773 UR - https://www.unboundmedicine.com/medline/citation/31568601/Exploring_the_Trans-Cleavage_Activity_of_CRISPR-Cas12a_(cpf1)_for_the_Development_of_a_Universal_Electrochemical_Biosensor L2 - https://doi.org/10.1002/anie.201910772 DB - PRIME DP - Unbound Medicine ER -