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Effect of chronic L-carnitine supplementation on carnitine levels, oxidative stress and apoptotic markers in peripheral organs of adult Wistar rats.
Food Chem Toxicol 2019; 134:110851FC

Abstract

This study investigated the effects of L-carnitine supplementation on carnitine levels, oxidative stress and apoptotic markers in the stomach, kidney, liver and testis tissues in adult rats. Rats were randomized to control and L-carnitine supplemented (LCAR) groups. Control group received distilled water for 7 months by intragastric gavage and the LCAR group was given 50 mg/kg/day L-carnitine via intragastric intubation for the same period. L-carnitine concentrations and caspase-3 activity were measured by fluorometric methods while cleaved caspase-3 was determined by Western blot analysis. Bcl-2 associated X protein (Bax) and B-cell lymphoma/leukemia-2 (Bcl-2) were quantified by enzyme immunoassay and Western blot analysis. Oxygen/nitrogen species (ROS/RNS) and total antioxidant capacity (TAC) were analyzed by colorimetric assay. Tissue L-carnitine concentrations were significantly increased in the LCAR group compared to controls. Anti-apoptotic Bcl-2 levels were significantly increased while pro-apoptotic Bax was significantly decreased in LCAR group rats compared to controls. Tissue caspase-3 was significantly alleviated in the LCAR group compared to controls. L-carnitine supplementation increased TAC and decreased ROS/RNS generation in the kidney, liver, stomach and testis tissues compared to controls. Obtained data suggests that L-carnitine supplementation can potentially be used to lessen both oxidative and apoptotic progression in peripheral organs.

Authors+Show Affiliations

Vocational School of Health Services, Akdeniz University, Antalya, 07070, Turkey. Electronic address: sevimercan@akdeniz.edu.tr.Department of Medical Biochemistry, Akdeniz University Medical School, Antalya, 07070, Turkey. Electronic address: ebrukiracc@gmail.com.Department of Biophysics, Akdeniz University Medical School, Antalya, 07070, Turkey. Electronic address: guvenakcayibu@gmail.com.Department of Biophysics, Akdeniz University Medical School, Antalya, 07070, Turkey. Electronic address: dbetul@akdeniz.edu.tr.Department of Medical Biochemistry, Akdeniz University Medical School, Antalya, 07070, Turkey. Electronic address: mutayaslan@akdeniz.edu.tr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31568849

Citation

Kelek, Sevim Ercan, et al. "Effect of Chronic L-carnitine Supplementation On Carnitine Levels, Oxidative Stress and Apoptotic Markers in Peripheral Organs of Adult Wistar Rats." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 134, 2019, p. 110851.
Kelek SE, Afşar E, Akçay G, et al. Effect of chronic L-carnitine supplementation on carnitine levels, oxidative stress and apoptotic markers in peripheral organs of adult Wistar rats. Food Chem Toxicol. 2019;134:110851.
Kelek, S. E., Afşar, E., Akçay, G., Danışman, B., & Aslan, M. (2019). Effect of chronic L-carnitine supplementation on carnitine levels, oxidative stress and apoptotic markers in peripheral organs of adult Wistar rats. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 134, p. 110851. doi:10.1016/j.fct.2019.110851.
Kelek SE, et al. Effect of Chronic L-carnitine Supplementation On Carnitine Levels, Oxidative Stress and Apoptotic Markers in Peripheral Organs of Adult Wistar Rats. Food Chem Toxicol. 2019;134:110851. PubMed PMID: 31568849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of chronic L-carnitine supplementation on carnitine levels, oxidative stress and apoptotic markers in peripheral organs of adult Wistar rats. AU - Kelek,Sevim Ercan, AU - Afşar,Ebru, AU - Akçay,Güven, AU - Danışman,Betül, AU - Aslan,Mutay, Y1 - 2019/09/27/ PY - 2019/06/24/received PY - 2019/09/22/revised PY - 2019/09/24/accepted PY - 2019/10/1/pubmed PY - 2019/10/1/medline PY - 2019/10/1/entrez KW - Apoptosis KW - L-carnitine KW - Oxidative stress SP - 110851 EP - 110851 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 134 N2 - This study investigated the effects of L-carnitine supplementation on carnitine levels, oxidative stress and apoptotic markers in the stomach, kidney, liver and testis tissues in adult rats. Rats were randomized to control and L-carnitine supplemented (LCAR) groups. Control group received distilled water for 7 months by intragastric gavage and the LCAR group was given 50 mg/kg/day L-carnitine via intragastric intubation for the same period. L-carnitine concentrations and caspase-3 activity were measured by fluorometric methods while cleaved caspase-3 was determined by Western blot analysis. Bcl-2 associated X protein (Bax) and B-cell lymphoma/leukemia-2 (Bcl-2) were quantified by enzyme immunoassay and Western blot analysis. Oxygen/nitrogen species (ROS/RNS) and total antioxidant capacity (TAC) were analyzed by colorimetric assay. Tissue L-carnitine concentrations were significantly increased in the LCAR group compared to controls. Anti-apoptotic Bcl-2 levels were significantly increased while pro-apoptotic Bax was significantly decreased in LCAR group rats compared to controls. Tissue caspase-3 was significantly alleviated in the LCAR group compared to controls. L-carnitine supplementation increased TAC and decreased ROS/RNS generation in the kidney, liver, stomach and testis tissues compared to controls. Obtained data suggests that L-carnitine supplementation can potentially be used to lessen both oxidative and apoptotic progression in peripheral organs. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/31568849/Effect_of_chronic_L-carnitine_supplementation_on_carnitine_levels,_oxidative_stress_and_apoptotic_markers_in_peripheral_organs_of_adult_Wistar_rats L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(19)30641-6 DB - PRIME DP - Unbound Medicine ER -