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FSH1 regulates the phenotype and pathogenicity of the pathogenic dermatophyte Microsporum canis.
Int J Mol Med 2019; 44(6):2047-2056IJ

Abstract

Microsporum canis (M. canis) is a common pathogen that causes tinea capitis and is present worldwide. The incidence of M. canis infection, particularly tinea capitis, has been increasing in China. In our previous studies, family of serine hydrolases 1 (FSH1) was identified as a potential virulence factor in tinea capitis infection caused by M. canis. To determine the function of this gene in M. canis, FSH1 was knocked down using double‑stranded RNA interference mediated by Agrobacterium tumefaciens. Reverse transcription‑quantitative PCR analysis was used to confirm gene knockdown. Loss of FSH1 expression by RNAi resulted in a minor phenotype alteration, but M. canis pathogenicity in guinea pig cutaneous infection was decreased compared with the wild‑type strain. To the best of our knowledge, the present study is the first to demonstrate that FSH1 is associated with macroconidia septa formation and is an important contributor to M. canis virulence. These findings may advance the understanding of the function of the FSH1 gene and provide a foundation for future studies on macroconidia septa formation and pathogenicity of M. canis.

Authors+Show Affiliations

Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Chengdu, Sichuan 11736, P.R. China.Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Chengdu, Sichuan 11736, P.R. China.Department of Dermatology, Hospital of Anjing Town, Chengdu, Sichuan 11736, P.R. China.Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Chengdu, Sichuan 11736, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31573050

Citation

Zhang, Furong, et al. "FSH1 Regulates the Phenotype and Pathogenicity of the Pathogenic Dermatophyte Microsporum Canis." International Journal of Molecular Medicine, vol. 44, no. 6, 2019, pp. 2047-2056.
Zhang F, Tan C, Xu Y, et al. FSH1 regulates the phenotype and pathogenicity of the pathogenic dermatophyte Microsporum canis. Int J Mol Med. 2019;44(6):2047-2056.
Zhang, F., Tan, C., Xu, Y., & Yang, G. (2019). FSH1 regulates the phenotype and pathogenicity of the pathogenic dermatophyte Microsporum canis. International Journal of Molecular Medicine, 44(6), pp. 2047-2056. doi:10.3892/ijmm.2019.4355.
Zhang F, et al. FSH1 Regulates the Phenotype and Pathogenicity of the Pathogenic Dermatophyte Microsporum Canis. Int J Mol Med. 2019;44(6):2047-2056. PubMed PMID: 31573050.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - FSH1 regulates the phenotype and pathogenicity of the pathogenic dermatophyte Microsporum canis. AU - Zhang,Furong, AU - Tan,Can, AU - Xu,Yu, AU - Yang,Guoling, Y1 - 2019/09/27/ PY - 2019/05/31/received PY - 2019/09/03/accepted PY - 2019/10/2/pubmed PY - 2019/10/2/medline PY - 2019/10/2/entrez SP - 2047 EP - 2056 JF - International journal of molecular medicine JO - Int. J. Mol. Med. VL - 44 IS - 6 N2 - Microsporum canis (M. canis) is a common pathogen that causes tinea capitis and is present worldwide. The incidence of M. canis infection, particularly tinea capitis, has been increasing in China. In our previous studies, family of serine hydrolases 1 (FSH1) was identified as a potential virulence factor in tinea capitis infection caused by M. canis. To determine the function of this gene in M. canis, FSH1 was knocked down using double‑stranded RNA interference mediated by Agrobacterium tumefaciens. Reverse transcription‑quantitative PCR analysis was used to confirm gene knockdown. Loss of FSH1 expression by RNAi resulted in a minor phenotype alteration, but M. canis pathogenicity in guinea pig cutaneous infection was decreased compared with the wild‑type strain. To the best of our knowledge, the present study is the first to demonstrate that FSH1 is associated with macroconidia septa formation and is an important contributor to M. canis virulence. These findings may advance the understanding of the function of the FSH1 gene and provide a foundation for future studies on macroconidia septa formation and pathogenicity of M. canis. SN - 1791-244X UR - https://www.unboundmedicine.com/medline/citation/31573050/FSH1_regulates_the_phenotype_and_pathogenicity_of_the_pathogenic_dermatophyte_Microsporum_canis L2 - http://www.spandidos-publications.com/ijmm/44/6/2047 DB - PRIME DP - Unbound Medicine ER -