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The LysR-type transcriptional regulator STM0030 contributes to Salmonella Typhimurium growth in macrophages and virulence in mice.
J Basic Microbiol. 2019 Nov; 59(11):1143-1153.JB

Abstract

Salmonella enterica serovar Typhimurium (S. Tm) is a major intracellular pathogen that infects humans and animals, and its survival and growth in macrophages is essential for its pathogenicity. More than 50 putative regulatory proteins are encoded by the S. Tm genome, but the functions of these regulatory proteins in mediating S. Tm pathogenicity are largely unknown. In this study, we investigated the biological function of the STM0030 gene, which encodes a putative LysR-type transcriptional regulator. We found that STM0030 is upregulated 2.8-5.7-fold during S. Tm growth in macrophages. Further, mutating this gene decreased bacterial growth in macrophages and attenuated virulence in mice. RNA-sequencing to investigate the regulatory function of STM0030 in S. Tm revealed that 447 genes were differentially expressed between the mutant and the wild-type strains; 429 of these genes were downregulated, suggesting that STM0030 mainly acts as a transcriptional activator. Moreover, the expression of gluconate, maltose, and hexose-p transport genes, as well as allantoin utilization genes were downregulated in the STM0030 mutant; this might be associated with the observed decrease in intracellular replication and pathogenicity of the mutant. Our findings suggest that STM0030 is a new pathogenicity-associated regulatory protein that broadens our understanding of the virulence regulatory network of S. Tm.

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Authors+Show Affiliations

Zhang H
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China. Key Laboratory of Microbial Functional Genomics, Tianjin, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, China. College of Life Sciences, Nankai University, Tianjin, China.
Song X
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China. Key Laboratory of Microbial Functional Genomics, Tianjin, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, China. College of Life Sciences, Nankai University, Tianjin, China.
Lv R
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China. Key Laboratory of Microbial Functional Genomics, Tianjin, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, China.
Liu X
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China. Key Laboratory of Microbial Functional Genomics, Tianjin, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, China.
Wang P
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China. Key Laboratory of Microbial Functional Genomics, Tianjin, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, China. College of Life Sciences, Nankai University, Tianjin, China.
Jiang L
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China. Key Laboratory of Microbial Functional Genomics, Tianjin, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, China.

MeSH

AnimalsBacterial ProteinsFemaleGene Expression Regulation, BacterialMacrophagesMiceMice, Inbred BALB CMutationRAW 264.7 CellsSalmonella Infections, AnimalSalmonella typhimuriumTranscription FactorsVirulenceVirulence Factors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31577373

Citation

Zhang, Huan, et al. "The LysR-type Transcriptional Regulator STM0030 Contributes to Salmonella Typhimurium Growth in Macrophages and Virulence in Mice." Journal of Basic Microbiology, vol. 59, no. 11, 2019, pp. 1143-1153.
Zhang H, Song X, Lv R, et al. The LysR-type transcriptional regulator STM0030 contributes to Salmonella Typhimurium growth in macrophages and virulence in mice. J Basic Microbiol. 2019;59(11):1143-1153.
Zhang, H., Song, X., Lv, R., Liu, X., Wang, P., & Jiang, L. (2019). The LysR-type transcriptional regulator STM0030 contributes to Salmonella Typhimurium growth in macrophages and virulence in mice. Journal of Basic Microbiology, 59(11), 1143-1153. https://doi.org/10.1002/jobm.201900315
Zhang H, et al. The LysR-type Transcriptional Regulator STM0030 Contributes to Salmonella Typhimurium Growth in Macrophages and Virulence in Mice. J Basic Microbiol. 2019;59(11):1143-1153. PubMed PMID: 31577373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The LysR-type transcriptional regulator STM0030 contributes to Salmonella Typhimurium growth in macrophages and virulence in mice. AU - Zhang,Huan, AU - Song,Xiaorui, AU - Lv,Runxia, AU - Liu,Xiaoqian, AU - Wang,Peisheng, AU - Jiang,Lingyan, Y1 - 2019/10/02/ PY - 2019/06/06/received PY - 2019/08/01/revised PY - 2019/08/29/accepted PY - 2019/10/3/pubmed PY - 2019/12/21/medline PY - 2019/10/3/entrez KW - RNA sequencing KW - STM0030 KW - Salmonella Typhimurium KW - intracellular replication KW - pathogenicity SP - 1143 EP - 1153 JF - Journal of basic microbiology JO - J Basic Microbiol VL - 59 IS - 11 N2 - Salmonella enterica serovar Typhimurium (S. Tm) is a major intracellular pathogen that infects humans and animals, and its survival and growth in macrophages is essential for its pathogenicity. More than 50 putative regulatory proteins are encoded by the S. Tm genome, but the functions of these regulatory proteins in mediating S. Tm pathogenicity are largely unknown. In this study, we investigated the biological function of the STM0030 gene, which encodes a putative LysR-type transcriptional regulator. We found that STM0030 is upregulated 2.8-5.7-fold during S. Tm growth in macrophages. Further, mutating this gene decreased bacterial growth in macrophages and attenuated virulence in mice. RNA-sequencing to investigate the regulatory function of STM0030 in S. Tm revealed that 447 genes were differentially expressed between the mutant and the wild-type strains; 429 of these genes were downregulated, suggesting that STM0030 mainly acts as a transcriptional activator. Moreover, the expression of gluconate, maltose, and hexose-p transport genes, as well as allantoin utilization genes were downregulated in the STM0030 mutant; this might be associated with the observed decrease in intracellular replication and pathogenicity of the mutant. Our findings suggest that STM0030 is a new pathogenicity-associated regulatory protein that broadens our understanding of the virulence regulatory network of S. Tm. SN - 1521-4028 UR - https://www.unboundmedicine.com/medline/citation/31577373/The_LysR_type_transcriptional_regulator_STM0030_contributes_to_Salmonella_Typhimurium_growth_in_macrophages_and_virulence_in_mice_ DB - PRIME DP - Unbound Medicine ER -