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LC-MS/MS quantitative analysis of phylloquinone, menaquinone-4 and menaquinone-7 in the human serum of a healthy population.
PeerJ 2019; 7:e7695P

Abstract

A novel application of the liquid chromatography method combined with the triple quadrupole tandem mass spectrometry method was developed for the quantification of vitamin K1 and two forms of vitamin K2 (menaquinone-4, menaquinone-7) in human serum. Total chromatography time for each run was 9 min. Time required for the sample pretreatment procedures was approximately 4 h. The coefficients of variation (CVs) of intra-assay were 10.4%, 3.2 % and 2.3% for vitamin K1 in three levels of quality control samples; were 14.3%, 3.2% and 6.7% for menaquinone-4; and were 11.1%, 6.0% and 7.0% for menaquinone-7. The inter-assay CVs were 12.8%, 11.3% and 7.4% for vitamin K1; were 15.2%, 9.2% and 8.7% for menaquinone-4; and were 13.2%,11.1% and 7.2% for menaquinone-7. No interference was found between K1, menaquinone-4 and menaquinone-7, nor any deuterated internal standards. This method was then used to determine reference values for Caucasian populations of central European origin. Samples were measured from 191 healthy volunteers (51.2 ± 16.2 years (mean ± SD)) and the values concerning K1 were 0.044-1.357 ng/mL for women and 0.030-1.214 ng/mL for men. The values for menaquinone-4 and menaquinone-7 did not exhibit any differences between women and men, and were 0.050-1.598 and 0.074-0.759 ng/mL, respectively.

Authors+Show Affiliations

Department of Medical Chemistry and Clinical Biochemistry, Charles University Prague, Second Faculty of Medicine and University Hospital Motol, Prague, Czech Republic.Department of Medical Chemistry and Clinical Biochemistry, Charles University Prague, Second Faculty of Medicine and University Hospital Motol, Prague, Czech Republic.Department of Medical Chemistry and Clinical Biochemistry, Charles University Prague, Second Faculty of Medicine and University Hospital Motol, Prague, Czech Republic.Department of Medical Chemistry and Clinical Biochemistry, Charles University Prague, Second Faculty of Medicine and University Hospital Motol, Prague, Czech Republic.Department of Medical Chemistry and Clinical Biochemistry, Charles University Prague, Second Faculty of Medicine and University Hospital Motol, Prague, Czech Republic.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31579595

Citation

Dunovska, Katerina, et al. "LC-MS/MS Quantitative Analysis of Phylloquinone, Menaquinone-4 and Menaquinone-7 in the Human Serum of a Healthy Population." PeerJ, vol. 7, 2019, pp. e7695.
Dunovska K, Klapkova E, Sopko B, et al. LC-MS/MS quantitative analysis of phylloquinone, menaquinone-4 and menaquinone-7 in the human serum of a healthy population. PeerJ. 2019;7:e7695.
Dunovska, K., Klapkova, E., Sopko, B., Cepova, J., & Prusa, R. (2019). LC-MS/MS quantitative analysis of phylloquinone, menaquinone-4 and menaquinone-7 in the human serum of a healthy population. PeerJ, 7, pp. e7695. doi:10.7717/peerj.7695.
Dunovska K, et al. LC-MS/MS Quantitative Analysis of Phylloquinone, Menaquinone-4 and Menaquinone-7 in the Human Serum of a Healthy Population. PeerJ. 2019;7:e7695. PubMed PMID: 31579595.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LC-MS/MS quantitative analysis of phylloquinone, menaquinone-4 and menaquinone-7 in the human serum of a healthy population. AU - Dunovska,Katerina, AU - Klapkova,Eva, AU - Sopko,Bruno, AU - Cepova,Jana, AU - Prusa,Richard, Y1 - 2019/09/19/ PY - 2019/03/07/received PY - 2019/08/19/accepted PY - 2019/10/4/entrez PY - 2019/10/4/pubmed PY - 2019/10/4/medline KW - Deuterated internal standard KW - LC–MS/MS KW - Menaquinone KW - Phylloquinone SP - e7695 EP - e7695 JF - PeerJ JO - PeerJ VL - 7 N2 - A novel application of the liquid chromatography method combined with the triple quadrupole tandem mass spectrometry method was developed for the quantification of vitamin K1 and two forms of vitamin K2 (menaquinone-4, menaquinone-7) in human serum. Total chromatography time for each run was 9 min. Time required for the sample pretreatment procedures was approximately 4 h. The coefficients of variation (CVs) of intra-assay were 10.4%, 3.2 % and 2.3% for vitamin K1 in three levels of quality control samples; were 14.3%, 3.2% and 6.7% for menaquinone-4; and were 11.1%, 6.0% and 7.0% for menaquinone-7. The inter-assay CVs were 12.8%, 11.3% and 7.4% for vitamin K1; were 15.2%, 9.2% and 8.7% for menaquinone-4; and were 13.2%,11.1% and 7.2% for menaquinone-7. No interference was found between K1, menaquinone-4 and menaquinone-7, nor any deuterated internal standards. This method was then used to determine reference values for Caucasian populations of central European origin. Samples were measured from 191 healthy volunteers (51.2 ± 16.2 years (mean ± SD)) and the values concerning K1 were 0.044-1.357 ng/mL for women and 0.030-1.214 ng/mL for men. The values for menaquinone-4 and menaquinone-7 did not exhibit any differences between women and men, and were 0.050-1.598 and 0.074-0.759 ng/mL, respectively. SN - 2167-8359 UR - https://www.unboundmedicine.com/medline/citation/31579595/LC-MS/MS_quantitative_analysis_of_phylloquinone,_menaquinone-4_and_menaquinone-7_in_the_human_serum_of_a_healthy_population L2 - https://doi.org/10.7717/peerj.7695 DB - PRIME DP - Unbound Medicine ER -