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Inulinase production and mathematical modeling from carob extract by using Aspergillus niger.
Biotechnol Prog. 2020 01; 36(1):e2919.BP

Abstract

The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium. In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase. For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4)2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed. After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract. As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively. Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively. Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source. Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.

Authors+Show Affiliations

Akdeniz University, Department of Food Engineering, Antalya, Turkey.Akdeniz University, Department of Food Engineering, Antalya, Turkey.Akdeniz University, Department of Food Engineering, Antalya, Turkey.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31581350

Citation

Ilgın, Merve, et al. "Inulinase Production and Mathematical Modeling From Carob Extract By Using Aspergillus Niger." Biotechnology Progress, vol. 36, no. 1, 2020, pp. e2919.
Ilgın M, Germec M, Turhan I. Inulinase production and mathematical modeling from carob extract by using Aspergillus niger. Biotechnol Prog. 2020;36(1):e2919.
Ilgın, M., Germec, M., & Turhan, I. (2020). Inulinase production and mathematical modeling from carob extract by using Aspergillus niger. Biotechnology Progress, 36(1), e2919. https://doi.org/10.1002/btpr.2919
Ilgın M, Germec M, Turhan I. Inulinase Production and Mathematical Modeling From Carob Extract By Using Aspergillus Niger. Biotechnol Prog. 2020;36(1):e2919. PubMed PMID: 31581350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inulinase production and mathematical modeling from carob extract by using Aspergillus niger. AU - Ilgın,Merve, AU - Germec,Mustafa, AU - Turhan,Irfan, Y1 - 2019/10/22/ PY - 2019/07/19/received PY - 2019/09/17/revised PY - 2019/09/22/accepted PY - 2019/10/4/pubmed PY - 2019/10/4/medline PY - 2019/10/4/entrez KW - Aspergillus KW - carob extract KW - flexible models KW - fungal inulinase KW - optimization SP - e2919 EP - e2919 JF - Biotechnology progress JO - Biotechnol. Prog. VL - 36 IS - 1 N2 - The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium. In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase. For medium optimization, eight different variables including initial sugar concentration (°Bx), (NH4)2 HPO4 , MgSO4 .7H2 O, KH2 PO4 , NH4 NO3 , yeast extract, peptone, and ZnSO4 .7H2 O were employed. After fermentations, optimum medium composition contained 1% yeast extract in 5°Bx carob extract. As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively. Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively. Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source. Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium. SN - 1520-6033 UR - https://www.unboundmedicine.com/medline/citation/31581350/Inulinase_production_and_mathematical_modeling_from_carob_extract_by_using_Aspergillus_niger_ L2 - https://doi.org/10.1002/btpr.2919 DB - PRIME DP - Unbound Medicine ER -
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