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Upregulated Expression of Macrophage Migration Inhibitory Factor, Its Analogue D-Dopachrome Tautomerase, and the CD44 Receptor in Peripheral CD4 T Cells from Clinically Isolated Syndrome Patients with Rapid Conversion to Clinical Defined Multiple Sclerosis.
Medicina (Kaunas) 2019; 55(10)M

Abstract

Background and objectives: Macrophage Migration Inhibitory Factor (MIF) and D-Dopachrome Tautomerase (DDT) are two pleiotropic and primarily, but not exclusively, proinflammatory cytokines belonging to the MIF family of cytokines that have recently been shown to be implicated in the pathogenesis of progressive forms of human progressive Multiple Sclerosis (MS) and the experimental model counterpart in rodents. Materials and

Methods:

We have presently evaluated a transcriptomic analysis of the expression of MIF, DDT, their receptors CD74 and CD44, and MIF co-receptors CXCR2, CXCR4, and CXCR7 in peripheral blood of patients with Clinically Isolated Syndrome (CIS), with rapid progression to clinical defined MS.

Results:

Our analysis reveals that MIF, DDT, and CD44 are overexpressed in CD4+ T cells from patients with CIS, as compared to healthy controls. Accordingly, a significant overlap was observed between the genes overexpressed in CD4+ T cells from patients with CIS and the genes belonging to the MIF regulatory network. This upregulated expression appeared to be unique for CD4+T cells, as other immune cells including CD8+ T cells, B cells, and monocytes from these patients exhibited expression levels of these molecules that were superimposable to those observed in healthy controls.

Conclusions:

Overall, our data suggest that the overexpression MIF cytokine family signature may occur in CD4+ T cells from patients with CIS, and that this phenomenon may be implicated in the pathogenesis of the disease, offering the possibility to represent both a diagnostic marker and a therapeutic target.

Authors+Show Affiliations

Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy. eugeniocavalli9@hotmail.it.IRCCS Centro Neurolesi Bonino Pulejo, C.da Casazza, 98124 Messina, Italy. emanuela.mazzon@irccsme.it.Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy. sofiabasile@hotmail.it.Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy. kmangano@unict.it.Department of Medicine and Health Sciences "Vincenzo Tiberio", University of Molise, 86100 Campobasso, Italy. roberto.dimarco@unimol.it.IRCCS Centro Neurolesi Bonino Pulejo, C.da Casazza, 98124 Messina, Italy. placido.bramanti@irccsmet.it.Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy. ferdinic@unict.it.Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy. paolofagone@yahoo.it.IRCCS Centro Neurolesi Bonino Pulejo, C.da Casazza, 98124 Messina, Italy. m.cristinapetralia@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31581595

Citation

Cavalli, Eugenio, et al. "Upregulated Expression of Macrophage Migration Inhibitory Factor, Its Analogue D-Dopachrome Tautomerase, and the CD44 Receptor in Peripheral CD4 T Cells From Clinically Isolated Syndrome Patients With Rapid Conversion to Clinical Defined Multiple Sclerosis." Medicina (Kaunas, Lithuania), vol. 55, no. 10, 2019.
Cavalli E, Mazzon E, Basile MS, et al. Upregulated Expression of Macrophage Migration Inhibitory Factor, Its Analogue D-Dopachrome Tautomerase, and the CD44 Receptor in Peripheral CD4 T Cells from Clinically Isolated Syndrome Patients with Rapid Conversion to Clinical Defined Multiple Sclerosis. Medicina (Kaunas). 2019;55(10).
Cavalli, E., Mazzon, E., Basile, M. S., Mangano, K., Di Marco, R., Bramanti, P., ... Petralia, M. C. (2019). Upregulated Expression of Macrophage Migration Inhibitory Factor, Its Analogue D-Dopachrome Tautomerase, and the CD44 Receptor in Peripheral CD4 T Cells from Clinically Isolated Syndrome Patients with Rapid Conversion to Clinical Defined Multiple Sclerosis. Medicina (Kaunas, Lithuania), 55(10), doi:10.3390/medicina55100667.
Cavalli E, et al. Upregulated Expression of Macrophage Migration Inhibitory Factor, Its Analogue D-Dopachrome Tautomerase, and the CD44 Receptor in Peripheral CD4 T Cells From Clinically Isolated Syndrome Patients With Rapid Conversion to Clinical Defined Multiple Sclerosis. Medicina (Kaunas). 2019 Oct 1;55(10) PubMed PMID: 31581595.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulated Expression of Macrophage Migration Inhibitory Factor, Its Analogue D-Dopachrome Tautomerase, and the CD44 Receptor in Peripheral CD4 T Cells from Clinically Isolated Syndrome Patients with Rapid Conversion to Clinical Defined Multiple Sclerosis. AU - Cavalli,Eugenio, AU - Mazzon,Emanuela, AU - Basile,Maria Sofia, AU - Mangano,Katia, AU - Di Marco,Roberto, AU - Bramanti,Placido, AU - Nicoletti,Ferdinando, AU - Fagone,Paolo, AU - Petralia,Maria Cristina, Y1 - 2019/10/01/ PY - 2019/09/11/received PY - 2019/09/26/revised PY - 2019/09/27/accepted PY - 2019/10/5/entrez KW - CD4+ T cells KW - d-dopachrome tautomerase KW - macrophage migration inhibitory factor KW - multiple sclerosis JF - Medicina (Kaunas, Lithuania) JO - Medicina (Kaunas) VL - 55 IS - 10 N2 - Background and objectives: Macrophage Migration Inhibitory Factor (MIF) and D-Dopachrome Tautomerase (DDT) are two pleiotropic and primarily, but not exclusively, proinflammatory cytokines belonging to the MIF family of cytokines that have recently been shown to be implicated in the pathogenesis of progressive forms of human progressive Multiple Sclerosis (MS) and the experimental model counterpart in rodents. Materials and Methods: We have presently evaluated a transcriptomic analysis of the expression of MIF, DDT, their receptors CD74 and CD44, and MIF co-receptors CXCR2, CXCR4, and CXCR7 in peripheral blood of patients with Clinically Isolated Syndrome (CIS), with rapid progression to clinical defined MS. Results: Our analysis reveals that MIF, DDT, and CD44 are overexpressed in CD4+ T cells from patients with CIS, as compared to healthy controls. Accordingly, a significant overlap was observed between the genes overexpressed in CD4+ T cells from patients with CIS and the genes belonging to the MIF regulatory network. This upregulated expression appeared to be unique for CD4+T cells, as other immune cells including CD8+ T cells, B cells, and monocytes from these patients exhibited expression levels of these molecules that were superimposable to those observed in healthy controls. Conclusions: Overall, our data suggest that the overexpression MIF cytokine family signature may occur in CD4+ T cells from patients with CIS, and that this phenomenon may be implicated in the pathogenesis of the disease, offering the possibility to represent both a diagnostic marker and a therapeutic target. SN - 1648-9144 UR - https://www.unboundmedicine.com/medline/citation/31581595/Upregulated_Expression_of_Macrophage_Migration_Inhibitory_Factor,_Its_Analogue_D-Dopachrome_Tautomerase,_and_the_CD44_Receptor_in_Peripheral_CD4_T_Cells_from_Clinically_Isolated_Syndrome_Patients_with_Rapid_Conversion_to_Clinical_Defined_Multiple_Sclerosis L2 - http://www.mdpi.com/resolver?pii=medicina55100667 DB - PRIME DP - Unbound Medicine ER -