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Physical Mapping of Stem Rust Resistance Gene Sr52 from Dasypyrum villosum Based on ph1b-Induced Homoeologous Recombination.
Int J Mol Sci. 2019 Oct 02; 20(19)IJ

Abstract

Wheat stem rust caused by Puccinia graminis f. sp. tritici (Pgt) had been a devastating foliar disease worldwide during the 20th century. With the emergence of Ug99 races, which are virulent to most stem rust resistance genes deployed in wheat varieties and advanced lines, stem rust has once again become a disease threatening global wheat production. Sr52, derived from Dasypyrum villosum and mapped to the long arm of 6V#3, is one of the few effective genes against Ug99 races. In this study, the wheat-D. villosum Robertsonian translocation T6AS·6V#3L, the only stock carrying Sr52 released to experimental and breeding programs so far, was crossed with a CS ph1b mutant to induce recombinants with shortened 6V#3L chromosome segments locating Sr52. Six independent homozygous recombinants with different segment sizes and breakpoints were developed and characterized using in situ hybridization and molecular markers analyses. Stem rust resistance evaluation showed that only three terminal recombinants (1381, 1380, and 1392) containing 8%, 22%, and 30% of the distal segment of 6V#3L, respectively, were resistant to stem rust. Thus, the gene Sr52 was mapped into 6V#3L bin FL 0.92-1.00. In addition, three molecular markers in the Sr52-located interval of 6V#3L were confirmed to be diagnostic markers for selection of Sr52 introgressed into common wheat. The newly developed small segment translocation lines with Sr52 and the identified molecular markers closely linked to Sr52 will be valuable for wheat disease breeding.

Authors+Show Affiliations

College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. lihuanhuanhappy@henau.edu.cn.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. zhenjiedong@hotmail.com.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. machao0813@hotmail.com.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. XiubinTian2019@hotmail.com.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. zjqi@njau.edu.cn.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. 2017201043@njau.edu.cn.Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA. friebe@ksu.edu.Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. xiangwheat@126.com.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. qingxia412@hotmail.com.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China. liuwenxuan@henau.edu.cn.College of Plant Protection, Shenyang Agricultural University, Shenyang 110000, China. litianya11@syau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31581639

Citation

Li, Huanhuan, et al. "Physical Mapping of Stem Rust Resistance Gene Sr52 From Dasypyrum Villosum Based On ph1b-Induced Homoeologous Recombination." International Journal of Molecular Sciences, vol. 20, no. 19, 2019.
Li H, Dong Z, Ma C, et al. Physical Mapping of Stem Rust Resistance Gene Sr52 from Dasypyrum villosum Based on ph1b-Induced Homoeologous Recombination. Int J Mol Sci. 2019;20(19).
Li, H., Dong, Z., Ma, C., Tian, X., Qi, Z., Wu, N., Friebe, B., Xiang, Z., Xia, Q., Liu, W., & Li, T. (2019). Physical Mapping of Stem Rust Resistance Gene Sr52 from Dasypyrum villosum Based on ph1b-Induced Homoeologous Recombination. International Journal of Molecular Sciences, 20(19). https://doi.org/10.3390/ijms20194887
Li H, et al. Physical Mapping of Stem Rust Resistance Gene Sr52 From Dasypyrum Villosum Based On ph1b-Induced Homoeologous Recombination. Int J Mol Sci. 2019 Oct 2;20(19) PubMed PMID: 31581639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physical Mapping of Stem Rust Resistance Gene Sr52 from Dasypyrum villosum Based on ph1b-Induced Homoeologous Recombination. AU - Li,Huanhuan, AU - Dong,Zhenjie, AU - Ma,Chao, AU - Tian,Xiubin, AU - Qi,Zengjun, AU - Wu,Nan, AU - Friebe,Bernd, AU - Xiang,Zhiguo, AU - Xia,Qing, AU - Liu,Wenxuan, AU - Li,Tianya, Y1 - 2019/10/02/ PY - 2019/09/18/received PY - 2019/09/30/revised PY - 2019/10/01/accepted PY - 2019/10/5/entrez PY - 2019/10/5/pubmed PY - 2020/2/13/medline KW - D. villosum KW - Sr52 KW - common wheat KW - molecular marker KW - small segment translocation line KW - stem rust resistance JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 19 N2 - Wheat stem rust caused by Puccinia graminis f. sp. tritici (Pgt) had been a devastating foliar disease worldwide during the 20th century. With the emergence of Ug99 races, which are virulent to most stem rust resistance genes deployed in wheat varieties and advanced lines, stem rust has once again become a disease threatening global wheat production. Sr52, derived from Dasypyrum villosum and mapped to the long arm of 6V#3, is one of the few effective genes against Ug99 races. In this study, the wheat-D. villosum Robertsonian translocation T6AS·6V#3L, the only stock carrying Sr52 released to experimental and breeding programs so far, was crossed with a CS ph1b mutant to induce recombinants with shortened 6V#3L chromosome segments locating Sr52. Six independent homozygous recombinants with different segment sizes and breakpoints were developed and characterized using in situ hybridization and molecular markers analyses. Stem rust resistance evaluation showed that only three terminal recombinants (1381, 1380, and 1392) containing 8%, 22%, and 30% of the distal segment of 6V#3L, respectively, were resistant to stem rust. Thus, the gene Sr52 was mapped into 6V#3L bin FL 0.92-1.00. In addition, three molecular markers in the Sr52-located interval of 6V#3L were confirmed to be diagnostic markers for selection of Sr52 introgressed into common wheat. The newly developed small segment translocation lines with Sr52 and the identified molecular markers closely linked to Sr52 will be valuable for wheat disease breeding. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31581639/Physical_Mapping_of_Stem_Rust_Resistance_Gene_Sr52_from_Dasypyrum_villosum_Based_on_ph1b_Induced_Homoeologous_Recombination_ L2 - https://www.mdpi.com/resolver?pii=ijms20194887 DB - PRIME DP - Unbound Medicine ER -