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Analysis of 3-indolylacetic acid and abscisic acid by high-performance liquid chromatography and gas-liquid chromatography.
Anal Biochem. 1985 Apr; 146(1):184-90.AB

Abstract

A method of analysis of 3-indolylacetic acid (IAA) and abscisic acid (ABA), allowing the simultaneous extraction of both regulators from plant material, has been developed. The method involves extraction with methanol, isolation of the acid fraction, diazomethane methylation, separation of the hormones through reverse-phase preparative high-performance liquid chromatography, and quantification of both compounds by gas-liquid chromatography. The recovery percentage at each step was monitored with radioactive compounds added at the beginning of the process. The final recovery was 70% for IAA and 96% for ABA. The method was applied to the analysis of the IAA and ABA content of stems of hazel (Corylus avellana L.).

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3158244

Citation

Rodriguez, A, and R S. Sanchez Tames. "Analysis of 3-indolylacetic Acid and Abscisic Acid By High-performance Liquid Chromatography and Gas-liquid Chromatography." Analytical Biochemistry, vol. 146, no. 1, 1985, pp. 184-90.
Rodriguez A, Sanchez Tames RS. Analysis of 3-indolylacetic acid and abscisic acid by high-performance liquid chromatography and gas-liquid chromatography. Anal Biochem. 1985;146(1):184-90.
Rodriguez, A., & Sanchez Tames, R. S. (1985). Analysis of 3-indolylacetic acid and abscisic acid by high-performance liquid chromatography and gas-liquid chromatography. Analytical Biochemistry, 146(1), 184-90.
Rodriguez A, Sanchez Tames RS. Analysis of 3-indolylacetic Acid and Abscisic Acid By High-performance Liquid Chromatography and Gas-liquid Chromatography. Anal Biochem. 1985;146(1):184-90. PubMed PMID: 3158244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of 3-indolylacetic acid and abscisic acid by high-performance liquid chromatography and gas-liquid chromatography. AU - Rodriguez,A, AU - Sanchez Tames,R S, PY - 1985/4/1/pubmed PY - 1985/4/1/medline PY - 1985/4/1/entrez SP - 184 EP - 90 JF - Analytical biochemistry JO - Anal Biochem VL - 146 IS - 1 N2 - A method of analysis of 3-indolylacetic acid (IAA) and abscisic acid (ABA), allowing the simultaneous extraction of both regulators from plant material, has been developed. The method involves extraction with methanol, isolation of the acid fraction, diazomethane methylation, separation of the hormones through reverse-phase preparative high-performance liquid chromatography, and quantification of both compounds by gas-liquid chromatography. The recovery percentage at each step was monitored with radioactive compounds added at the beginning of the process. The final recovery was 70% for IAA and 96% for ABA. The method was applied to the analysis of the IAA and ABA content of stems of hazel (Corylus avellana L.). SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/3158244/Analysis_of_3_indolylacetic_acid_and_abscisic_acid_by_high_performance_liquid_chromatography_and_gas_liquid_chromatography_ DB - PRIME DP - Unbound Medicine ER -