Tags

Type your tag names separated by a space and hit enter

Blastomyces dermatitidis Antibody and Antigen Detection: Comparison of Four Lysate Antigens and Antibodies Prepared from Human Isolates from a Blastomycosis Outbreak.
Mycopathologia. 2019 Oct; 184(5):661-666.M

Abstract

Blastomycosis is a systemic fungal disease of humans and other animals produced by the thermally dimorphic fungal organism, Blastomyces dermatitidis. Recent studies have focused on the utilization of antibody and antigen detection in the development of immunoassays for the diagnosis of blastomycosis. This study was designed to evaluate four B. dermatitidis yeast lysate antigenic preparations from human isolates (591, 592, 597, 598) from an outbreak of blastomycosis in Eagle River, Wisconsin. The indirect enzyme-linked immunosorbent assay (ELISA) was used to compare these four antigens for their ability to detect antibodies in 28 serum specimens from immunized rabbits and in 18 sera from dogs with blastomycosis. This study also compared antibodies prepared from each of the four B. dermatitidis lysate antigens for their ability to detect antigen using the competitive enzyme-linked immunosorbent assay in 18 urine specimens from the same dogs as above with blastomycosis. All four reagents proved to be immunoreactive and were able to detect antibody in the rabbit and dog sera and antigen in each of the urine specimens with only slight variations in the mean absorbance values evidenced. Antibody detection, mean absorbance values with the four lysates, ranged from 1.522 (592 antigen) to 2.047 (597 antigen) in the rabbit sera and from 1.504 (591 antigen) to 1.878 (597 antigen) in the dog sera. Antigen detection, sensitivity values obtained with the antibodies prepared from the four lysates, ranged from 89% (598 serum) to 100% (591 and 592 serum specimens).

Authors+Show Affiliations

Department of Biological Sciences, Idaho State University, Pocatello, ID, USA.Department of Biological Sciences, Idaho State University, Pocatello, ID, USA.Department of Biological Sciences, Idaho State University, Pocatello, ID, USA. scalgene@isu.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31583528

Citation

Hammon, Jordan T., et al. "Blastomyces Dermatitidis Antibody and Antigen Detection: Comparison of Four Lysate Antigens and Antibodies Prepared From Human Isolates From a Blastomycosis Outbreak." Mycopathologia, vol. 184, no. 5, 2019, pp. 661-666.
Hammon JT, Kennell BC, Scalarone GM. Blastomyces dermatitidis Antibody and Antigen Detection: Comparison of Four Lysate Antigens and Antibodies Prepared from Human Isolates from a Blastomycosis Outbreak. Mycopathologia. 2019;184(5):661-666.
Hammon, J. T., Kennell, B. C., & Scalarone, G. M. (2019). Blastomyces dermatitidis Antibody and Antigen Detection: Comparison of Four Lysate Antigens and Antibodies Prepared from Human Isolates from a Blastomycosis Outbreak. Mycopathologia, 184(5), 661-666. https://doi.org/10.1007/s11046-019-00385-0
Hammon JT, Kennell BC, Scalarone GM. Blastomyces Dermatitidis Antibody and Antigen Detection: Comparison of Four Lysate Antigens and Antibodies Prepared From Human Isolates From a Blastomycosis Outbreak. Mycopathologia. 2019;184(5):661-666. PubMed PMID: 31583528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blastomyces dermatitidis Antibody and Antigen Detection: Comparison of Four Lysate Antigens and Antibodies Prepared from Human Isolates from a Blastomycosis Outbreak. AU - Hammon,Jordan T, AU - Kennell,Bryn C, AU - Scalarone,Gene M, Y1 - 2019/10/03/ PY - 2018/04/30/received PY - 2019/09/14/accepted PY - 2019/10/5/pubmed PY - 2020/4/9/medline PY - 2019/10/5/entrez KW - Antibody detection KW - Antigen detection KW - Blastomyces dermatitidis KW - ELISA KW - Lysate antigens SP - 661 EP - 666 JF - Mycopathologia JO - Mycopathologia VL - 184 IS - 5 N2 - Blastomycosis is a systemic fungal disease of humans and other animals produced by the thermally dimorphic fungal organism, Blastomyces dermatitidis. Recent studies have focused on the utilization of antibody and antigen detection in the development of immunoassays for the diagnosis of blastomycosis. This study was designed to evaluate four B. dermatitidis yeast lysate antigenic preparations from human isolates (591, 592, 597, 598) from an outbreak of blastomycosis in Eagle River, Wisconsin. The indirect enzyme-linked immunosorbent assay (ELISA) was used to compare these four antigens for their ability to detect antibodies in 28 serum specimens from immunized rabbits and in 18 sera from dogs with blastomycosis. This study also compared antibodies prepared from each of the four B. dermatitidis lysate antigens for their ability to detect antigen using the competitive enzyme-linked immunosorbent assay in 18 urine specimens from the same dogs as above with blastomycosis. All four reagents proved to be immunoreactive and were able to detect antibody in the rabbit and dog sera and antigen in each of the urine specimens with only slight variations in the mean absorbance values evidenced. Antibody detection, mean absorbance values with the four lysates, ranged from 1.522 (592 antigen) to 2.047 (597 antigen) in the rabbit sera and from 1.504 (591 antigen) to 1.878 (597 antigen) in the dog sera. Antigen detection, sensitivity values obtained with the antibodies prepared from the four lysates, ranged from 89% (598 serum) to 100% (591 and 592 serum specimens). SN - 1573-0832 UR - https://www.unboundmedicine.com/medline/citation/31583528/Blastomyces_dermatitidis_Antibody_and_Antigen_Detection:_Comparison_of_Four_Lysate_Antigens_and_Antibodies_Prepared_from_Human_Isolates_from_a_Blastomycosis_Outbreak_ L2 - https://dx.doi.org/10.1007/s11046-019-00385-0 DB - PRIME DP - Unbound Medicine ER -