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Untargeted and Targeted Discrimination of Honey Collected by Apis cerana and Apis mellifera Based on Volatiles Using HS-GC-IMS and HS-SPME-GC-MS.
J Agric Food Chem. 2019 Oct 30; 67(43):12144-12152.JA

Abstract

Fraudulent acts regarding honey authenticity that use Apis mellifera honey as a substitute for Apis cerana honey have garnered considerable concern in China and triggered a trust crisis from consumers. In this study, untargeted metabolomics analysis was carried out based on volatile fractions in honey from A. cerana and A. mellifera using headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). Honey from A. cerana and A. mellifera was discriminated by HS-GC-IMS profiling, principal component analysis, and orthogonal partial least-squares discrimination analysis. Tentative markers were identified from p-values and the variable importance in projection analysis and confirmed using the retention index, mass fragments, and reference standards by gas chromatography-mass spectrometry (GC-MS). A targeted method was established using the headspace solid phase coupled with microextraction GC-MS (HS-SPME-GC-MS) to quantitate the markers. The results demonstrated that the developed untargeted and targeted metabolomics approach performed well when discriminating honey from A. cerana and A. mellifera.

Authors+Show Affiliations

Institute of Apicultural Research , Chinese Academy of Agricultural Sciences , Beijing 100093 , PR China.National Isotope Centre , GNS Science , 30 Gracefield Road , Lower Hutt 5040 , New Zealand.Institute of Apicultural Research , Chinese Academy of Agricultural Sciences , Beijing 100093 , PR China.Institute of Apicultural Research , Chinese Academy of Agricultural Sciences , Beijing 100093 , PR China.Institute of Apicultural Research , Chinese Academy of Agricultural Sciences , Beijing 100093 , PR China.Institute of Apicultural Research , Chinese Academy of Agricultural Sciences , Beijing 100093 , PR China.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

31587558

Citation

Wang, Xinran, et al. "Untargeted and Targeted Discrimination of Honey Collected By Apis Cerana and Apis Mellifera Based On Volatiles Using HS-GC-IMS and HS-SPME-GC-MS." Journal of Agricultural and Food Chemistry, vol. 67, no. 43, 2019, pp. 12144-12152.
Wang X, Rogers KM, Li Y, et al. Untargeted and Targeted Discrimination of Honey Collected by Apis cerana and Apis mellifera Based on Volatiles Using HS-GC-IMS and HS-SPME-GC-MS. J Agric Food Chem. 2019;67(43):12144-12152.
Wang, X., Rogers, K. M., Li, Y., Yang, S., Chen, L., & Zhou, J. (2019). Untargeted and Targeted Discrimination of Honey Collected by Apis cerana and Apis mellifera Based on Volatiles Using HS-GC-IMS and HS-SPME-GC-MS. Journal of Agricultural and Food Chemistry, 67(43), 12144-12152. https://doi.org/10.1021/acs.jafc.9b04438
Wang X, et al. Untargeted and Targeted Discrimination of Honey Collected By Apis Cerana and Apis Mellifera Based On Volatiles Using HS-GC-IMS and HS-SPME-GC-MS. J Agric Food Chem. 2019 Oct 30;67(43):12144-12152. PubMed PMID: 31587558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Untargeted and Targeted Discrimination of Honey Collected by Apis cerana and Apis mellifera Based on Volatiles Using HS-GC-IMS and HS-SPME-GC-MS. AU - Wang,Xinran, AU - Rogers,Karyne M, AU - Li,Yi, AU - Yang,Shupeng, AU - Chen,Lanzhen, AU - Zhou,Jinhui, Y1 - 2019/10/18/ PY - 2019/10/8/pubmed PY - 2020/1/3/medline PY - 2019/10/8/entrez KW - HS-GC-IMS KW - HS-SPME-GC−MS KW - honey KW - untargeted and targeted discrimination KW - volatiles markers SP - 12144 EP - 12152 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 67 IS - 43 N2 - Fraudulent acts regarding honey authenticity that use Apis mellifera honey as a substitute for Apis cerana honey have garnered considerable concern in China and triggered a trust crisis from consumers. In this study, untargeted metabolomics analysis was carried out based on volatile fractions in honey from A. cerana and A. mellifera using headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). Honey from A. cerana and A. mellifera was discriminated by HS-GC-IMS profiling, principal component analysis, and orthogonal partial least-squares discrimination analysis. Tentative markers were identified from p-values and the variable importance in projection analysis and confirmed using the retention index, mass fragments, and reference standards by gas chromatography-mass spectrometry (GC-MS). A targeted method was established using the headspace solid phase coupled with microextraction GC-MS (HS-SPME-GC-MS) to quantitate the markers. The results demonstrated that the developed untargeted and targeted metabolomics approach performed well when discriminating honey from A. cerana and A. mellifera. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/31587558/Untargeted_and_Targeted_Discrimination_of_Honey_Collected_by_Apis_cerana_and_Apis_mellifera_Based_on_Volatiles_Using_HS_GC_IMS_and_HS_SPME_GC_MS_ DB - PRIME DP - Unbound Medicine ER -