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Spontaneous pain-associated facial expression and efficacy of clinically used drugs in the reserpine-induced rat model of fibromyalgia.
Eur J Pharmacol. 2019 Dec 01; 864:172716.EJ

Abstract

Fibromyalgia-associated chronic pain occurring without organic causes exerts negative effects on patients' quality of life, thereby necessitating the development of superior drugs. Since non-organic pain in patients with fibromyalgia occurs without external stimuli, an endpoint measure that reflects patients' spontaneous pain should be implemented in preclinical research. The present study is the first to apply the rat grimace scale (RGS), a facial expression-dependent measure developed for quantifying spontaneous pain, to the rat with reserpine-induced myalgia, an animal model of fibromyalgia exhibiting non-organic pain. Animals were videotaped and still images of facial expressions were captured and scored in a blind fashion. The reserpine-induced myalgia rats exhibited a significant increase in the RGS score, which was sustained for 2 weeks or more after the induction of fibromyalgia-like state by reserpine injection. The period of RGS score elevation was similar to that of reduced paw withdrawal threshold (PWT) measured using the von Frey filament test, a conventional measure of evoked pain. The elevated RGS score and the decreased PWT were relieved by gabapentin (an α2δ subunit ligand) and duloxetine (a serotonin and noradrenaline reuptake inhibitor), but not by diclofenac (a nonsteroidal anti-inflammatory drug), buprenorphine (a mu-opioid receptor agonist), or diazepam (a benzodiazepine). The present study suggests that facial expressions in reserpine-induced myalgia rats simulate non-organic pain occurring spontaneously in patients with fibromyalgia. This finding achieves a coordination of pain measures between the animal model and patients with fibromyalgia and would improve the translation of analgesic efficacies between them.

Authors+Show Affiliations

Faculty of Pharmaceutical Sciences, Aomori University, 2-3-1 Kohbata, Aomori-city, Aomori, 030-0943, Japan; School of Pharmacy, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara-city, Tochigi, 324-8501, Japan. Electronic address: nagakurayu@iuhw.ac.jp.Faculty of Pharmaceutical Sciences, Aomori University, 2-3-1 Kohbata, Aomori-city, Aomori, 030-0943, Japan.Faculty of Pharmaceutical Sciences, Aomori University, 2-3-1 Kohbata, Aomori-city, Aomori, 030-0943, Japan.Faculty of Pharmaceutical Sciences, Aomori University, 2-3-1 Kohbata, Aomori-city, Aomori, 030-0943, Japan.Faculty of Pharmaceutical Sciences, Aomori University, 2-3-1 Kohbata, Aomori-city, Aomori, 030-0943, Japan; Faculty of Pharmaceutical Sciences, Nihon Pharmaceutical University, 10281 Komuro, Ina-machi, Kitaadachi-gun, Saitama, 362-0806, Japan.School of Pharmacy, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara-city, Tochigi, 324-8501, Japan.School of Pharmacy, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara-city, Tochigi, 324-8501, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31589868

Citation

Nagakura, Yukinori, et al. "Spontaneous Pain-associated Facial Expression and Efficacy of Clinically Used Drugs in the Reserpine-induced Rat Model of Fibromyalgia." European Journal of Pharmacology, vol. 864, 2019, p. 172716.
Nagakura Y, Miwa M, Yoshida M, et al. Spontaneous pain-associated facial expression and efficacy of clinically used drugs in the reserpine-induced rat model of fibromyalgia. Eur J Pharmacol. 2019;864:172716.
Nagakura, Y., Miwa, M., Yoshida, M., Miura, R., Tanei, S., Tsuji, M., & Takeda, H. (2019). Spontaneous pain-associated facial expression and efficacy of clinically used drugs in the reserpine-induced rat model of fibromyalgia. European Journal of Pharmacology, 864, 172716. https://doi.org/10.1016/j.ejphar.2019.172716
Nagakura Y, et al. Spontaneous Pain-associated Facial Expression and Efficacy of Clinically Used Drugs in the Reserpine-induced Rat Model of Fibromyalgia. Eur J Pharmacol. 2019 Dec 1;864:172716. PubMed PMID: 31589868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spontaneous pain-associated facial expression and efficacy of clinically used drugs in the reserpine-induced rat model of fibromyalgia. AU - Nagakura,Yukinori, AU - Miwa,Machiko, AU - Yoshida,Miku, AU - Miura,Reina, AU - Tanei,Shigeharu, AU - Tsuji,Minoru, AU - Takeda,Hiroshi, Y1 - 2019/10/04/ PY - 2019/07/04/received PY - 2019/09/20/revised PY - 2019/10/01/accepted PY - 2019/10/8/pubmed PY - 2020/3/26/medline PY - 2019/10/8/entrez KW - Analgesic efficacy translation KW - Animal model KW - Facial expression KW - Fibromyalgia KW - Non-organic pain KW - Spontaneous pain measure SP - 172716 EP - 172716 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 864 N2 - Fibromyalgia-associated chronic pain occurring without organic causes exerts negative effects on patients' quality of life, thereby necessitating the development of superior drugs. Since non-organic pain in patients with fibromyalgia occurs without external stimuli, an endpoint measure that reflects patients' spontaneous pain should be implemented in preclinical research. The present study is the first to apply the rat grimace scale (RGS), a facial expression-dependent measure developed for quantifying spontaneous pain, to the rat with reserpine-induced myalgia, an animal model of fibromyalgia exhibiting non-organic pain. Animals were videotaped and still images of facial expressions were captured and scored in a blind fashion. The reserpine-induced myalgia rats exhibited a significant increase in the RGS score, which was sustained for 2 weeks or more after the induction of fibromyalgia-like state by reserpine injection. The period of RGS score elevation was similar to that of reduced paw withdrawal threshold (PWT) measured using the von Frey filament test, a conventional measure of evoked pain. The elevated RGS score and the decreased PWT were relieved by gabapentin (an α2δ subunit ligand) and duloxetine (a serotonin and noradrenaline reuptake inhibitor), but not by diclofenac (a nonsteroidal anti-inflammatory drug), buprenorphine (a mu-opioid receptor agonist), or diazepam (a benzodiazepine). The present study suggests that facial expressions in reserpine-induced myalgia rats simulate non-organic pain occurring spontaneously in patients with fibromyalgia. This finding achieves a coordination of pain measures between the animal model and patients with fibromyalgia and would improve the translation of analgesic efficacies between them. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/31589868/Spontaneous_pain_associated_facial_expression_and_efficacy_of_clinically_used_drugs_in_the_reserpine_induced_rat_model_of_fibromyalgia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(19)30668-5 DB - PRIME DP - Unbound Medicine ER -