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Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians.
Nutrients. 2019 Oct 06; 11(10)N

Abstract

Lactovegetarians (n = 35) with low vitamin B12 (B12) status were intervened for eight weeks capsules containing cyano-B12 (CN-B12), (2 × 2.8 µg/day), or equivalent doses of endogenous B12 (mainly hydroxo-B12 (HO-B12)) in whey powder. Blood samples were examined at baseline, every second week during the intervention, and two weeks post-intervention. The groups did not differ at baseline in [global median (min/max)] plasma B12 [112(61/185)] pmol/L, holotranscobalamin [20(4/99)] pmol/L, folate [13(11/16)], the metabolites total homocysteine [18(9/52)] µmol/L and methylmalonic acid [0.90(0.28/2.5)] µmol/L, and the combined indicator of B12 status (4cB12) [-1.7(-3.0/-0.33)]. Both supplements caused significant effects, though none of the biomarkers returned to normal values. Total plasma B12 showed a higher increase in the capsule group compared to the whey powder group (p = 0.02). However, the increase of plasma holotranscobalamin (p = 0.06) and the lowering of the metabolites (p > 0.07) were alike in both groups. Thereby, the high total plasma B12 in the capsule group was not mirrored in enhanced B12 metabolism, possibly because the B12 surplus was mainly accumulated on an "inert" carrier haptocorrin, considered to be of marginal importance for tissue delivery of B12. In conclusion, we demonstrate that administration of whey powder (HO-B12) or capsules (CN-B12) equivalent to 5.6 µg of B12 daily for eight weeks similarly improves B12 status but does not normalize it. We document that the results for plasma B12 should be interpreted with caution following administration of CN-B12, since the change is disproportionately high compared to the responses of complementary biomarkers.

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Authors+Show Affiliations

Naik S
Department of Pathology, Deenanath Mangeshkar Hospital and Research Center, Pune, 411004, India. sadu_naik@rediffmail.com.
Mahalle N
Department of Pathology, Deenanath Mangeshkar Hospital and Research Center, Pune, 411004, India. pnmahalle@gmail.com.
Greibe E
Department of Clinical Biochemistry and Institute of Clinical Medicine, Aarhus University Hospital, DK-8200 Aarhus N, Denmark. evagreibe@gmail.com.
Ostenfeld MS
Arla Foods Ingredients Group P/S, DK-8260 Viby, Denmark. marie.stampe.ostenfeld@arlafoods.com.
Heegaard CW
Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus C, Denmark. cwh@mbg.au.dk.
Nexo E
Department of Clinical Biochemistry and Institute of Clinical Medicine, Aarhus University Hospital, DK-8200 Aarhus N, Denmark. ebbanexo@rm.dk.
Fedosov SN
Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus C, Denmark. snf@mbg.au.dk.

MeSH

AdolescentAdultBiomarkersDiet, VegetarianDietary SupplementsFemaleHumansIndiaMaleMiddle AgedNutritional StatusPowdersTime FactorsTreatment OutcomeVegetariansVitamin B 12Vitamin B 12 DeficiencyWhey ProteinsYoung Adult

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

31590426

Citation

Naik, Sadanand, et al. "Cyano-B12 or Whey Powder With Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians." Nutrients, vol. 11, no. 10, 2019.
Naik S, Mahalle N, Greibe E, et al. Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians. Nutrients. 2019;11(10).
Naik, S., Mahalle, N., Greibe, E., Ostenfeld, M. S., Heegaard, C. W., Nexo, E., & Fedosov, S. N. (2019). Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians. Nutrients, 11(10). https://doi.org/10.3390/nu11102382
Naik S, et al. Cyano-B12 or Whey Powder With Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians. Nutrients. 2019 Oct 6;11(10) PubMed PMID: 31590426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians. AU - Naik,Sadanand, AU - Mahalle,Namita, AU - Greibe,Eva, AU - Ostenfeld,Marie S, AU - Heegaard,Christian W, AU - Nexo,Ebba, AU - Fedosov,Sergey N, Y1 - 2019/10/06/ PY - 2019/08/12/received PY - 2019/09/18/revised PY - 2019/09/19/accepted PY - 2019/10/9/entrez PY - 2019/10/9/pubmed PY - 2020/3/5/medline KW - B12 deficiency KW - B12 supplementation KW - Vitamin B12 KW - cyano-B12 KW - hydroxo-B12 KW - lactovegetarians KW - milk KW - whey powder JF - Nutrients JO - Nutrients VL - 11 IS - 10 N2 - Lactovegetarians (n = 35) with low vitamin B12 (B12) status were intervened for eight weeks capsules containing cyano-B12 (CN-B12), (2 × 2.8 µg/day), or equivalent doses of endogenous B12 (mainly hydroxo-B12 (HO-B12)) in whey powder. Blood samples were examined at baseline, every second week during the intervention, and two weeks post-intervention. The groups did not differ at baseline in [global median (min/max)] plasma B12 [112(61/185)] pmol/L, holotranscobalamin [20(4/99)] pmol/L, folate [13(11/16)], the metabolites total homocysteine [18(9/52)] µmol/L and methylmalonic acid [0.90(0.28/2.5)] µmol/L, and the combined indicator of B12 status (4cB12) [-1.7(-3.0/-0.33)]. Both supplements caused significant effects, though none of the biomarkers returned to normal values. Total plasma B12 showed a higher increase in the capsule group compared to the whey powder group (p = 0.02). However, the increase of plasma holotranscobalamin (p = 0.06) and the lowering of the metabolites (p > 0.07) were alike in both groups. Thereby, the high total plasma B12 in the capsule group was not mirrored in enhanced B12 metabolism, possibly because the B12 surplus was mainly accumulated on an "inert" carrier haptocorrin, considered to be of marginal importance for tissue delivery of B12. In conclusion, we demonstrate that administration of whey powder (HO-B12) or capsules (CN-B12) equivalent to 5.6 µg of B12 daily for eight weeks similarly improves B12 status but does not normalize it. We document that the results for plasma B12 should be interpreted with caution following administration of CN-B12, since the change is disproportionately high compared to the responses of complementary biomarkers. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/31590426/Cyano_B12_or_Whey_Powder_with_Endogenous_Hydroxo_B12_for_Supplementation_in_B12_Deficient_Lactovegetarians_ DB - PRIME DP - Unbound Medicine ER -