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Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles.
J Agric Food Chem. 2019 Dec 11; 67(49):13367-13392.JA

Abstract

Green leaf volatiles (GLVs) are mainly C6- and in rare cases also C9-aldehydes, -alcohols, and -esters, which are released by plants in response to biotic or abiotic stresses. These compounds are named for their characteristic smell reminiscent of freshly mowed grass. This review focuses on GLVs and the two major pathway enzymes responsible for their formation: lipoxygenases (LOXs) and fatty acid hydroperoxide lyases (HPLs). LOXs catalyze the peroxidation of unsaturated fatty acids, such as linoleic and α-linolenic acids. Hydroperoxy fatty acids are further converted by HPLs into aldehydes and oxo-acids. In many industrial applications, plant extracts have been used as LOX and HPL sources. However, these processes are limited by low enzyme concentration, stability, and specificity. Alternatively, recombinant enzymes can be used as biocatalysts for GLV synthesis. The increasing number of well-characterized enzymes efficiently expressed by microbial hosts will foster the development of innovative biocatalytic processes for GLV production.

Authors+Show Affiliations

Austrian Centre of Industrial Biotechnology , Petersgasse 14 , 8010 Graz , Austria.Austrian Centre of Industrial Biotechnology , Petersgasse 14 , 8010 Graz , Austria. bisy e.U. , Wetzawinkel 20 , 8200 Hofstaetten , Austria.Austrian Centre of Industrial Biotechnology , Petersgasse 14 , 8010 Graz , Austria. Institute of Molecular Biotechnology , TU Graz, NAWI Graz, BioTechMed Graz , Petersgasse 14 , 8010 Graz , Austria.Austrian Centre of Industrial Biotechnology , Petersgasse 14 , 8010 Graz , Austria. Institute of Molecular Biotechnology , TU Graz, NAWI Graz, BioTechMed Graz , Petersgasse 14 , 8010 Graz , Austria.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

31591878

Citation

Stolterfoht, Holly, et al. "Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles." Journal of Agricultural and Food Chemistry, vol. 67, no. 49, 2019, pp. 13367-13392.
Stolterfoht H, Rinnofner C, Winkler M, et al. Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles. J Agric Food Chem. 2019;67(49):13367-13392.
Stolterfoht, H., Rinnofner, C., Winkler, M., & Pichler, H. (2019). Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles. Journal of Agricultural and Food Chemistry, 67(49), 13367-13392. https://doi.org/10.1021/acs.jafc.9b02690
Stolterfoht H, et al. Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles. J Agric Food Chem. 2019 Dec 11;67(49):13367-13392. PubMed PMID: 31591878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles. AU - Stolterfoht,Holly, AU - Rinnofner,Claudia, AU - Winkler,Margit, AU - Pichler,Harald, Y1 - 2019/11/25/ PY - 2019/10/9/pubmed PY - 2019/12/24/medline PY - 2019/10/9/entrez KW - fatty acid hydroperoxide lyase KW - green leaf volatiles KW - heterologous host organism (bacteria, yeasts, and plant cells) KW - lipoxygenase KW - natural flavor production KW - recombinant enzymes SP - 13367 EP - 13392 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 67 IS - 49 N2 - Green leaf volatiles (GLVs) are mainly C6- and in rare cases also C9-aldehydes, -alcohols, and -esters, which are released by plants in response to biotic or abiotic stresses. These compounds are named for their characteristic smell reminiscent of freshly mowed grass. This review focuses on GLVs and the two major pathway enzymes responsible for their formation: lipoxygenases (LOXs) and fatty acid hydroperoxide lyases (HPLs). LOXs catalyze the peroxidation of unsaturated fatty acids, such as linoleic and α-linolenic acids. Hydroperoxy fatty acids are further converted by HPLs into aldehydes and oxo-acids. In many industrial applications, plant extracts have been used as LOX and HPL sources. However, these processes are limited by low enzyme concentration, stability, and specificity. Alternatively, recombinant enzymes can be used as biocatalysts for GLV synthesis. The increasing number of well-characterized enzymes efficiently expressed by microbial hosts will foster the development of innovative biocatalytic processes for GLV production. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/31591878/Recombinant_Lipoxygenases_and_Hydroperoxide_Lyases_for_the_Synthesis_of_Green_Leaf_Volatiles_ DB - PRIME DP - Unbound Medicine ER -