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Comparison of carpet and toothbrush techniques for the detection of Microsporum canis in cats.
J Feline Med Surg 2019; :1098612X19880632JF

Abstract

OBJECTIVES

The aim of this study was to evaluate the diagnostic concordance between the toothbrush and carpet techniques for the detection of Microsporum canis in cats in a field study.

METHODS

Thirty-nine Persian cats from a cattery were used. Fungal culture samples from the haircoat of each cat were collected by stroking the coat with a sterile toothbrush and a 5 × 5 cm-sized sterile carpet square (n = 78 total samples). Specimens were inoculated onto Mycosel Agar and incubated at 25°C for 21 days. Both techniques were compared using the following parameters: number of plates without fungal growth, number of plates with contaminant growth and number of plates positive for dermatophytes.

RESULTS

The feline population in the study cattery was 39. Thirty (77%) were symptomatic and nine (23%) asymptomatic. The diagnosis was made via carpet and toothbrush methods and 78 cultures were performed. On day 21, M canis was detected in all culture plates. No contaminant molds were observed.

CONCLUSIONS AND RELEVANCE

The concordance rate between the carpet and toothbrush techniques among the 78 evaluable culture plates was 100%. Both methods are equally effective for collecting material for M canis culture. Additionally, both techniques are inexpensive and easy to perform in feline clinical practice.

Authors+Show Affiliations

Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Laboratory of Medical Mycology, Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil.Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31592711

Citation

Santana, Aline E., et al. "Comparison of Carpet and Toothbrush Techniques for the Detection of Microsporum Canis in Cats." Journal of Feline Medicine and Surgery, 2019, p. 1098612X19880632.
Santana AE, Taborda CP, Filgueira KD, et al. Comparison of carpet and toothbrush techniques for the detection of Microsporum canis in cats. J Feline Med Surg. 2019.
Santana, A. E., Taborda, C. P., Filgueira, K. D., Sellera, F. P., Larsson, C. E., & Reche-Junior, A. (2019). Comparison of carpet and toothbrush techniques for the detection of Microsporum canis in cats. Journal of Feline Medicine and Surgery, p. 1098612X19880632. doi:10.1177/1098612X19880632.
Santana AE, et al. Comparison of Carpet and Toothbrush Techniques for the Detection of Microsporum Canis in Cats. J Feline Med Surg. 2019 Oct 8;1098612X19880632. PubMed PMID: 31592711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of carpet and toothbrush techniques for the detection of Microsporum canis in cats. AU - Santana,Aline E, AU - Taborda,Carlos P, AU - Filgueira,Kilder D, AU - Sellera,Fábio P, AU - Larsson,Carlos E, AU - Reche-Junior,Archivaldo, Y1 - 2019/10/08/ PY - 2019/10/9/entrez KW - Microsporum canis KW - Persian KW - Toothbrush KW - cattery KW - dermatophytosis KW - fungal cultures SP - 1098612X19880632 EP - 1098612X19880632 JF - Journal of feline medicine and surgery JO - J. Feline Med. Surg. N2 - OBJECTIVES: The aim of this study was to evaluate the diagnostic concordance between the toothbrush and carpet techniques for the detection of Microsporum canis in cats in a field study. METHODS: Thirty-nine Persian cats from a cattery were used. Fungal culture samples from the haircoat of each cat were collected by stroking the coat with a sterile toothbrush and a 5 × 5 cm-sized sterile carpet square (n = 78 total samples). Specimens were inoculated onto Mycosel Agar and incubated at 25°C for 21 days. Both techniques were compared using the following parameters: number of plates without fungal growth, number of plates with contaminant growth and number of plates positive for dermatophytes. RESULTS: The feline population in the study cattery was 39. Thirty (77%) were symptomatic and nine (23%) asymptomatic. The diagnosis was made via carpet and toothbrush methods and 78 cultures were performed. On day 21, M canis was detected in all culture plates. No contaminant molds were observed. CONCLUSIONS AND RELEVANCE: The concordance rate between the carpet and toothbrush techniques among the 78 evaluable culture plates was 100%. Both methods are equally effective for collecting material for M canis culture. Additionally, both techniques are inexpensive and easy to perform in feline clinical practice. SN - 1532-2750 UR - https://www.unboundmedicine.com/medline/citation/31592711/Comparison_of_carpet_and_toothbrush_techniques_for_the_detection_of_Microsporum_canis_in_cats L2 - http://journals.sagepub.com/doi/full/10.1177/1098612X19880632?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -