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Isolation of Yasminevirus, the First Member of Klosneuvirinae Isolated in Coculture with Vermamoeba vermiformis, Demonstrates an Extended Arsenal of Translational Apparatus Components.
J Virol. 2019 12 12; 94(1)JV

Abstract

The family of giant viruses is still expanding, and evidence of a translational machinery is emerging in the virosphere. The Klosneuvirinae group of giant viruses was first reconstructed from in silico studies, and then a unique member was isolated, Bodo saltans virus. Here we describe the isolation of a new member in this group using coculture with the free-living amoeba Vermamoeba vermiformis This giant virus, called Yasminevirus, has a 2.1-Mb linear double-stranded DNA genome encoding 1,541 candidate proteins, with a GC content estimated at 40.2%. Yasminevirus possesses a nearly complete translational machinery, with a set of 70 tRNAs associated with 45 codons and recognizing 20 amino acids (aa), 20 aminoacyl-tRNA synthetases (aaRSs) recognizing 20 aa, as well as several translation factors and elongation factors. At the genome scale, evolutionary analyses placed this virus in the Klosneuvirinae group of giant viruses. Rhizome analysis demonstrated that the genome of Yasminevirus is mosaic, with ∼34% of genes having their closest homologues in other viruses, followed by ∼13.2% in Eukaryota, ∼7.2% in Bacteria, and less than 1% in Archaea Among giant virus sequences, Yasminevirus shared 87% of viral hits with Klosneuvirinae. This description of Yasminevirus sheds light on the Klosneuvirinae group in a captivating quest to understand the evolution and diversity of giant viruses.IMPORTANCE Yasminevirus is an icosahedral double-stranded DNA virus isolated from sewage water by amoeba coculture. Here its structure and replicative cycle in the amoeba Vermamoeba vermiformis are described and genomic and evolutionary studies are reported. This virus belongs to the Klosneuvirinae group of giant viruses, representing the second isolated and cultivated giant virus in this group, and is the first isolated using a coculture procedure. Extended translational machinery pointed to Yasminevirus among the quasiautonomous giant viruses with the most complete translational apparatus of the known virosphere.

Authors+Show Affiliations

Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia. Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. Aix-Marseille Université, MEPHI (Microbes, Evolution, Phylogeny and Infections), IRD, APHM, Faculté de Médecine, Marseille, France.Aix-Marseille Université, MEPHI (Microbes, Evolution, Phylogeny and Infections), IRD, APHM, Faculté de Médecine, Marseille, France.Aix-Marseille Université, MEPHI (Microbes, Evolution, Phylogeny and Infections), IRD, APHM, Faculté de Médecine, Marseille, France.Aix-Marseille Université, MEPHI (Microbes, Evolution, Phylogeny and Infections), IRD, APHM, Faculté de Médecine, Marseille, France.Aix-Marseille Université, MEPHI (Microbes, Evolution, Phylogeny and Infections), IRD, APHM, Faculté de Médecine, Marseille, France.Aix-Marseille Université, MEPHI (Microbes, Evolution, Phylogeny and Infections), IRD, APHM, Faculté de Médecine, Marseille, France. IHU Méditerranée Infection, Marseille, France.Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.Aix-Marseille Université, MEPHI (Microbes, Evolution, Phylogeny and Infections), IRD, APHM, Faculté de Médecine, Marseille, France bernard.la-scola@univ-amu.fr anthony.levasseur@univ-amu.fr. IHU Méditerranée Infection, Marseille, France.Aix-Marseille Université, MEPHI (Microbes, Evolution, Phylogeny and Infections), IRD, APHM, Faculté de Médecine, Marseille, France bernard.la-scola@univ-amu.fr anthony.levasseur@univ-amu.fr. IHU Méditerranée Infection, Marseille, France. Institut Universitaire de France, Paris, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31597770

Citation

Bajrai, Leena Hussein, et al. "Isolation of Yasminevirus, the First Member of Klosneuvirinae Isolated in Coculture With Vermamoeba Vermiformis, Demonstrates an Extended Arsenal of Translational Apparatus Components." Journal of Virology, vol. 94, no. 1, 2019.
Bajrai LH, Mougari S, Andreani J, et al. Isolation of Yasminevirus, the First Member of Klosneuvirinae Isolated in Coculture with Vermamoeba vermiformis, Demonstrates an Extended Arsenal of Translational Apparatus Components. J Virol. 2019;94(1).
Bajrai, L. H., Mougari, S., Andreani, J., Baptiste, E., Delerce, J., Raoult, D., Azhar, E. I., La Scola, B., & Levasseur, A. (2019). Isolation of Yasminevirus, the First Member of Klosneuvirinae Isolated in Coculture with Vermamoeba vermiformis, Demonstrates an Extended Arsenal of Translational Apparatus Components. Journal of Virology, 94(1). https://doi.org/10.1128/JVI.01534-19
Bajrai LH, et al. Isolation of Yasminevirus, the First Member of Klosneuvirinae Isolated in Coculture With Vermamoeba Vermiformis, Demonstrates an Extended Arsenal of Translational Apparatus Components. J Virol. 2019 12 12;94(1) PubMed PMID: 31597770.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of Yasminevirus, the First Member of Klosneuvirinae Isolated in Coculture with Vermamoeba vermiformis, Demonstrates an Extended Arsenal of Translational Apparatus Components. AU - Bajrai,Leena Hussein, AU - Mougari,Saïd, AU - Andreani,Julien, AU - Baptiste,Emeline, AU - Delerce,Jeremy, AU - Raoult,Didier, AU - Azhar,Esam Ibraheem, AU - La Scola,Bernard, AU - Levasseur,Anthony, Y1 - 2019/12/12/ PY - 2019/09/09/received PY - 2019/09/24/accepted PY - 2019/10/11/pubmed PY - 2020/6/12/medline PY - 2019/10/11/entrez KW - Klosneuvirinae KW - NCLDV KW - amoeba KW - giant virus KW - translation JF - Journal of virology JO - J Virol VL - 94 IS - 1 N2 - The family of giant viruses is still expanding, and evidence of a translational machinery is emerging in the virosphere. The Klosneuvirinae group of giant viruses was first reconstructed from in silico studies, and then a unique member was isolated, Bodo saltans virus. Here we describe the isolation of a new member in this group using coculture with the free-living amoeba Vermamoeba vermiformis This giant virus, called Yasminevirus, has a 2.1-Mb linear double-stranded DNA genome encoding 1,541 candidate proteins, with a GC content estimated at 40.2%. Yasminevirus possesses a nearly complete translational machinery, with a set of 70 tRNAs associated with 45 codons and recognizing 20 amino acids (aa), 20 aminoacyl-tRNA synthetases (aaRSs) recognizing 20 aa, as well as several translation factors and elongation factors. At the genome scale, evolutionary analyses placed this virus in the Klosneuvirinae group of giant viruses. Rhizome analysis demonstrated that the genome of Yasminevirus is mosaic, with ∼34% of genes having their closest homologues in other viruses, followed by ∼13.2% in Eukaryota, ∼7.2% in Bacteria, and less than 1% in Archaea Among giant virus sequences, Yasminevirus shared 87% of viral hits with Klosneuvirinae. This description of Yasminevirus sheds light on the Klosneuvirinae group in a captivating quest to understand the evolution and diversity of giant viruses.IMPORTANCE Yasminevirus is an icosahedral double-stranded DNA virus isolated from sewage water by amoeba coculture. Here its structure and replicative cycle in the amoeba Vermamoeba vermiformis are described and genomic and evolutionary studies are reported. This virus belongs to the Klosneuvirinae group of giant viruses, representing the second isolated and cultivated giant virus in this group, and is the first isolated using a coculture procedure. Extended translational machinery pointed to Yasminevirus among the quasiautonomous giant viruses with the most complete translational apparatus of the known virosphere. SN - 1098-5514 UR - https://www.unboundmedicine.com/medline/citation/31597770/Isolation_of_Yasminevirus_the_First_Member_of_Klosneuvirinae_Isolated_in_Coculture_with_Vermamoeba_vermiformis_Demonstrates_an_Extended_Arsenal_of_Translational_Apparatus_Components_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31597770/ DB - PRIME DP - Unbound Medicine ER -