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gas1 mutation extends chronological lifespan via Pmk1 and Sty1 MAPKs in Schizosaccharomyces pombe.
Biosci Biotechnol Biochem. 2020 Feb; 84(2):330-337.BB

Abstract

In the longevity research by using yeasts, chronological lifespan is defined as the survival time after entry into stationary phase. Previously, screening for long lived mutants of Schizosaccharomyces pombe was performed to identify the novel factors involved in longevity. From this screening, one long lived mutant called as No.36 was obtained. In this study, we identified the mutation caused in gas1+, which encodes glucanosyltransferase (gas1-287 mutation) is responsible for the longevity of No.36 mutant. Through the analysis of this mutant, we found that cell wall perturbing agent micafungin also extends chronological lifespan in fission yeast. This lifespan extension depended on both Pmk1 and Sty1 MAP kinases, and longevity caused by the gas1-287 mutation also depended on these kinases. In summary, we propose that the gas1-287 mutation causes longevity as the similar mechanism as cell wall stress depending on Pmk1 and Sty1 MAPK pathways.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, Japan.Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, Japan.Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31601154

Citation

Imai, Yuki, et al. "Gas1 Mutation Extends Chronological Lifespan Via Pmk1 and Sty1 MAPKs in Schizosaccharomyces Pombe." Bioscience, Biotechnology, and Biochemistry, vol. 84, no. 2, 2020, pp. 330-337.
Imai Y, Shimasaki T, Enokimura C, et al. Gas1 mutation extends chronological lifespan via Pmk1 and Sty1 MAPKs in Schizosaccharomyces pombe. Biosci Biotechnol Biochem. 2020;84(2):330-337.
Imai, Y., Shimasaki, T., Enokimura, C., Ohtsuka, H., Tsubouchi, S., Ihara, K., & Aiba, H. (2020). Gas1 mutation extends chronological lifespan via Pmk1 and Sty1 MAPKs in Schizosaccharomyces pombe. Bioscience, Biotechnology, and Biochemistry, 84(2), 330-337. https://doi.org/10.1080/09168451.2019.1676695
Imai Y, et al. Gas1 Mutation Extends Chronological Lifespan Via Pmk1 and Sty1 MAPKs in Schizosaccharomyces Pombe. Biosci Biotechnol Biochem. 2020;84(2):330-337. PubMed PMID: 31601154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - gas1 mutation extends chronological lifespan via Pmk1 and Sty1 MAPKs in Schizosaccharomyces pombe. AU - Imai,Yuki, AU - Shimasaki,Takafumi, AU - Enokimura,Chihiro, AU - Ohtsuka,Hokuto, AU - Tsubouchi,Satoshi, AU - Ihara,Kunio, AU - Aiba,Hirofumi, Y1 - 2019/10/10/ PY - 2019/10/12/pubmed PY - 2020/3/4/medline PY - 2019/10/12/entrez KW - Chronological lifespan KW - Gas1 KW - S. pombe KW - cell wall KW - longevity SP - 330 EP - 337 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 84 IS - 2 N2 - In the longevity research by using yeasts, chronological lifespan is defined as the survival time after entry into stationary phase. Previously, screening for long lived mutants of Schizosaccharomyces pombe was performed to identify the novel factors involved in longevity. From this screening, one long lived mutant called as No.36 was obtained. In this study, we identified the mutation caused in gas1+, which encodes glucanosyltransferase (gas1-287 mutation) is responsible for the longevity of No.36 mutant. Through the analysis of this mutant, we found that cell wall perturbing agent micafungin also extends chronological lifespan in fission yeast. This lifespan extension depended on both Pmk1 and Sty1 MAP kinases, and longevity caused by the gas1-287 mutation also depended on these kinases. In summary, we propose that the gas1-287 mutation causes longevity as the similar mechanism as cell wall stress depending on Pmk1 and Sty1 MAPK pathways. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/31601154/gas1_mutation_extends_chronological_lifespan_via_Pmk1_and_Sty1_MAPKs_in_Schizosaccharomyces_pombe_ L2 - https://www.tandfonline.com/doi/full/10.1080/09168451.2019.1676695 DB - PRIME DP - Unbound Medicine ER -