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IFCC-HbA1c May Be More Useful than NGSP-HbA1c in the Comparison between HbA1c Values in Variant Hemoglobin with a Mutation on α Chain and β Chain.
Ann Clin Lab Sci 2019; 49(5):650-655AC

Abstract

HbA1c values in variant hemoglobin with a mutation on α chain and β chain are assumed to be different because the α chain and β chain of the globin gene have two and one gene(s), respectively. We examined whether the differentiation between HbA1c values in variant hemoglobin with a mutation on α chain (C1α) and β chain (C1β) is possible. Three patients with C1α and 9 patients with C1β were included. HbA1c was measured by standard mode high-performance liquid chromatography (HPLC) (HPLC-HbA1c) and immunoassay (IA: IA-HbA1c). Glycated albumin (GA) was determined, and each ratio was compared. HbA1c is expressed in National Glycohemoglobin Standardization Program (NGSP) units (A1C) and International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) units (iA1c). Both the HPLC-A1C/IA-A1C ratio and the HPLC-iA1c/IA-iA1c ratio in C1α were significantly lower than those in C1β. Although there were no significant differences between the GA/HPLC-A1C ratios in both groups, the GA/HPLC-iA1c ratio in C1α was significantly higher than those in C1β. If the cutoff values for the HPLC/IA-iA1c ratio and GA/HPLC-iA1c ratio were set at 65% and 5.3, respectively, both sensitivity and specificity were 100%. Differentiation between C1α and C1β might be possible by using the HPLC-iA1c/IA-iA1c ratio or GA/HPLC-iA1c ratio.

Authors+Show Affiliations

Department of Internal Medicine, Hakuhokai Central Hospital, Japan m-koga@kawanishi-city-hospital.com.Department of Internal Medicine, Kawanishi City Hospital, Japan.Division of Diabetes and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.Department of Clinical Laboratory Medicine, Hyogo College of Medicine, Hyogo, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31611209

Citation

Koga, Masafumi, et al. "IFCC-HbA1c May Be More Useful Than NGSP-HbA1c in the Comparison Between HbA1c Values in Variant Hemoglobin With a Mutation On Α Chain and Β Chain." Annals of Clinical and Laboratory Science, vol. 49, no. 5, 2019, pp. 650-655.
Koga M, Inada S, Yoshino K, et al. IFCC-HbA1c May Be More Useful than NGSP-HbA1c in the Comparison between HbA1c Values in Variant Hemoglobin with a Mutation on α Chain and β Chain. Ann Clin Lab Sci. 2019;49(5):650-655.
Koga, M., Inada, S., Yoshino, K., & Miyazaki, A. (2019). IFCC-HbA1c May Be More Useful than NGSP-HbA1c in the Comparison between HbA1c Values in Variant Hemoglobin with a Mutation on α Chain and β Chain. Annals of Clinical and Laboratory Science, 49(5), pp. 650-655.
Koga M, et al. IFCC-HbA1c May Be More Useful Than NGSP-HbA1c in the Comparison Between HbA1c Values in Variant Hemoglobin With a Mutation On Α Chain and Β Chain. Ann Clin Lab Sci. 2019;49(5):650-655. PubMed PMID: 31611209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IFCC-HbA1c May Be More Useful than NGSP-HbA1c in the Comparison between HbA1c Values in Variant Hemoglobin with a Mutation on α Chain and β Chain. AU - Koga,Masafumi, AU - Inada,Shinya, AU - Yoshino,Kei, AU - Miyazaki,Ayako, PY - 2019/10/16/entrez PY - 2019/10/16/pubmed PY - 2019/10/16/medline KW - Globin gene KW - Glycated albumin KW - HbA1c KW - High-performance liquid chromatography KW - Variant hemoglobin SP - 650 EP - 655 JF - Annals of clinical and laboratory science JO - Ann. Clin. Lab. Sci. VL - 49 IS - 5 N2 - HbA1c values in variant hemoglobin with a mutation on α chain and β chain are assumed to be different because the α chain and β chain of the globin gene have two and one gene(s), respectively. We examined whether the differentiation between HbA1c values in variant hemoglobin with a mutation on α chain (C1α) and β chain (C1β) is possible. Three patients with C1α and 9 patients with C1β were included. HbA1c was measured by standard mode high-performance liquid chromatography (HPLC) (HPLC-HbA1c) and immunoassay (IA: IA-HbA1c). Glycated albumin (GA) was determined, and each ratio was compared. HbA1c is expressed in National Glycohemoglobin Standardization Program (NGSP) units (A1C) and International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) units (iA1c). Both the HPLC-A1C/IA-A1C ratio and the HPLC-iA1c/IA-iA1c ratio in C1α were significantly lower than those in C1β. Although there were no significant differences between the GA/HPLC-A1C ratios in both groups, the GA/HPLC-iA1c ratio in C1α was significantly higher than those in C1β. If the cutoff values for the HPLC/IA-iA1c ratio and GA/HPLC-iA1c ratio were set at 65% and 5.3, respectively, both sensitivity and specificity were 100%. Differentiation between C1α and C1β might be possible by using the HPLC-iA1c/IA-iA1c ratio or GA/HPLC-iA1c ratio. SN - 1550-8080 UR - https://www.unboundmedicine.com/medline/citation/31611209/IFCC-HbA1c_May_Be_More_Useful_than_NGSP-HbA1c_in_the_Comparison_between_HbA1c_Values_in_Variant_Hemoglobin_with_a_Mutation_on_α_Chain_and_β_Chain L2 - http://www.annclinlabsci.org/cgi/pmidlookup?view=long&pmid=31611209 DB - PRIME DP - Unbound Medicine ER -