Tags

Type your tag names separated by a space and hit enter

The Hippo pathway integrates PI3K-Akt signals with mechanical and polarity cues to control tissue growth.
PLoS Biol 2019; 17(10):e3000509PB

Abstract

The Hippo signalling pathway restricts cell proliferation in animal tissues by inhibiting Yes-associated protein (YAP or YAP1) and Transcriptional Activator with a PDZ domain (TAZ or WW-domain-containing transcriptional activator [WWTR1]), coactivators of the Scalloped (Sd or TEAD) DNA-binding transcription factor. Drosophila has a single YAP/TAZ homolog named Yorkie (Yki) that is regulated by Hippo pathway signalling in response to epithelial polarity and tissue mechanics during development. Here, we show that Yki translocates to the nucleus to drive Sd-mediated cell proliferation in the ovarian follicle cell epithelium in response to mechanical stretching caused by the growth of the germline. Importantly, mechanically induced Yki nuclear localisation also requires nutritionally induced insulin/insulin-like growth factor 1 (IGF-1) signalling (IIS) via phosphatidyl inositol-3-kinase (PI3K), phosphoinositide-dependent kinase 1 (PDK1 or PDPK1), and protein kinase B (Akt or PKB) in the follicular epithelium. We find similar results in the developing Drosophila wing, where Yki becomes nuclear in the mechanically stretched cells of the wing pouch during larval feeding, which induces IIS, but translocates to the cytoplasm upon cessation of feeding in the third instar stage. Inactivating Akt prevents nuclear Yki localisation in the wing disc, while ectopic activation of the insulin receptor, PI3K, or Akt/PKB is sufficient to maintain nuclear Yki in mechanically stimulated cells of the wing pouch even after feeding ceases. Finally, IIS also promotes YAP nuclear localisation in response to mechanical cues in mammalian skin epithelia. Thus, the Hippo pathway has a physiological function as an integrator of epithelial cell polarity, tissue mechanics, and nutritional cues to control cell proliferation and tissue growth in both Drosophila and mammals.

Authors+Show Affiliations

Epithelial Biology Laboratory, The Francis Crick Institute, London, United Kingdom.Epithelial Biology Laboratory, The Francis Crick Institute, London, United Kingdom.Epithelial Biology Laboratory, The Francis Crick Institute, London, United Kingdom.Epithelial Biology Laboratory, The Francis Crick Institute, London, United Kingdom.Epithelial Biology Laboratory, The Francis Crick Institute, London, United Kingdom.Epithelial Biology Laboratory, The Francis Crick Institute, London, United Kingdom. EMBL Australia, Department of Cancer Biology & Therapeutics, The John Curtin School of Medical Research, The Australian National University, Acton, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31613895

Citation

Borreguero-Muñoz, Nerea, et al. "The Hippo Pathway Integrates PI3K-Akt Signals With Mechanical and Polarity Cues to Control Tissue Growth." PLoS Biology, vol. 17, no. 10, 2019, pp. e3000509.
Borreguero-Muñoz N, Fletcher GC, Aguilar-Aragon M, et al. The Hippo pathway integrates PI3K-Akt signals with mechanical and polarity cues to control tissue growth. PLoS Biol. 2019;17(10):e3000509.
Borreguero-Muñoz, N., Fletcher, G. C., Aguilar-Aragon, M., Elbediwy, A., Vincent-Mistiaen, Z. I., & Thompson, B. J. (2019). The Hippo pathway integrates PI3K-Akt signals with mechanical and polarity cues to control tissue growth. PLoS Biology, 17(10), pp. e3000509. doi:10.1371/journal.pbio.3000509.
Borreguero-Muñoz N, et al. The Hippo Pathway Integrates PI3K-Akt Signals With Mechanical and Polarity Cues to Control Tissue Growth. PLoS Biol. 2019;17(10):e3000509. PubMed PMID: 31613895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Hippo pathway integrates PI3K-Akt signals with mechanical and polarity cues to control tissue growth. AU - Borreguero-Muñoz,Nerea, AU - Fletcher,Georgina C, AU - Aguilar-Aragon,Mario, AU - Elbediwy,Ahmed, AU - Vincent-Mistiaen,Zoé I, AU - Thompson,Barry J, Y1 - 2019/10/15/ PY - 2018/11/22/received PY - 2019/10/03/accepted PY - 2019/10/25/revised PY - 2019/10/16/pubmed PY - 2019/10/16/medline PY - 2019/10/16/entrez SP - e3000509 EP - e3000509 JF - PLoS biology JO - PLoS Biol. VL - 17 IS - 10 N2 - The Hippo signalling pathway restricts cell proliferation in animal tissues by inhibiting Yes-associated protein (YAP or YAP1) and Transcriptional Activator with a PDZ domain (TAZ or WW-domain-containing transcriptional activator [WWTR1]), coactivators of the Scalloped (Sd or TEAD) DNA-binding transcription factor. Drosophila has a single YAP/TAZ homolog named Yorkie (Yki) that is regulated by Hippo pathway signalling in response to epithelial polarity and tissue mechanics during development. Here, we show that Yki translocates to the nucleus to drive Sd-mediated cell proliferation in the ovarian follicle cell epithelium in response to mechanical stretching caused by the growth of the germline. Importantly, mechanically induced Yki nuclear localisation also requires nutritionally induced insulin/insulin-like growth factor 1 (IGF-1) signalling (IIS) via phosphatidyl inositol-3-kinase (PI3K), phosphoinositide-dependent kinase 1 (PDK1 or PDPK1), and protein kinase B (Akt or PKB) in the follicular epithelium. We find similar results in the developing Drosophila wing, where Yki becomes nuclear in the mechanically stretched cells of the wing pouch during larval feeding, which induces IIS, but translocates to the cytoplasm upon cessation of feeding in the third instar stage. Inactivating Akt prevents nuclear Yki localisation in the wing disc, while ectopic activation of the insulin receptor, PI3K, or Akt/PKB is sufficient to maintain nuclear Yki in mechanically stimulated cells of the wing pouch even after feeding ceases. Finally, IIS also promotes YAP nuclear localisation in response to mechanical cues in mammalian skin epithelia. Thus, the Hippo pathway has a physiological function as an integrator of epithelial cell polarity, tissue mechanics, and nutritional cues to control cell proliferation and tissue growth in both Drosophila and mammals. SN - 1545-7885 UR - https://www.unboundmedicine.com/medline/citation/31613895/The_Hippo_pathway_integrates_PI3K-Akt_signals_with_mechanical_and_polarity_cues_to_control_tissue_growth L2 - http://dx.plos.org/10.1371/journal.pbio.3000509 DB - PRIME DP - Unbound Medicine ER -