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Evaluation of synergism and development of a formulation with thymol, carvacrol and eugenol for Rhipicephalus microplus control.
Exp Parasitol. 2019 Dec; 207:107774.EP

Abstract

The acaricidal activity of combinations of thymol, carvacrol and eugenol was evaluated on larvae and engorged females of the cattle tick Rhipicephalus microplus. The first step assessed the compounds separately, in concentrations of 3.125, 6.25, 12.5 and 25 mg/mL. Then tests were performed with the compounds combined in the ratio of 1:1 at concentrations of 3.125 and 6.25 mg/mL, along with the control group treated with the solvent (3% DMSO). In the second step, combinations were tested incorporated in a formulation at the concentration de 6.25 mg/mL, using the larval packet and adult immersion tests. The associations carvacrol + thymol (3.125 mg/mL), carvacrol + eugenol and thymol + eugenol (6.25 mg/mL) presented synergism, while the other associations had an additive effect. In the experiments with formulation, all combinations caused 100% larval mortality, but the efficacy was under 15% against engorged females. Therefore, the combinations of thymol + carvacrol (3.125 mg/mL) as well as carvacrol + eugenol and eugenol + thymol (6.25 mg/mL) had a synergistic effect on engorged females, but when incorporated in the formulation, the acaricide activity was strong against larvae but weak against engorged females.

Authors+Show Affiliations

Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro (UFRRJ), BR-465, Km 7, Seropédica, RJ, 23890-000, Brazil.Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro (UFRRJ), BR-465, Km 7, Seropédica, RJ, 23890-000, Brazil.Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Bairro Martelos, CEP 36036-330, Juiz de Fora, MG, Laboratório de Artrópodes Zooparasitos, Brazil.Embrapa Gado de Leite, Rua Eugênio do Nascimento, 610, Dom Bosco, Juiz de Fora, MG, 36038-330, Laboratório de Parasitologia Brazil.Embrapa Gado de Leite, Rua Eugênio do Nascimento, 610, Dom Bosco, Juiz de Fora, MG, 36038-330, Laboratório de Parasitologia Brazil.Faculdade de Farmácia, Departamento de Ciências Farmacêuticas, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Bairro Martelos, CEP 36036-330, Juiz de Fora, MG, Brazil.Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Bairro Martelos, CEP 36036-330, Juiz de Fora, MG, Laboratório de Artrópodes Zooparasitos, Brazil.Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Bairro Martelos, CEP 36036-330, Juiz de Fora, MG, Laboratório de Artrópodes Zooparasitos, Brazil.Instituto de Patologia Tropical e Saúde Pública da Universidade Federal de Goiás (UFG), Rua 235, s/n, Setor Universitário, Goiânia, Goiás, 74605-050, Laboratório de Biologia, Ecologia e Controle de Carrapatos, Brazil. Electronic address: caiosat@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31614118

Citation

Novato, Tatiane Lopes Pinheiro, et al. "Evaluation of Synergism and Development of a Formulation With Thymol, Carvacrol and Eugenol for Rhipicephalus Microplus Control." Experimental Parasitology, vol. 207, 2019, p. 107774.
Novato TLP, Marchesini P, Muniz N, et al. Evaluation of synergism and development of a formulation with thymol, carvacrol and eugenol for Rhipicephalus microplus control. Exp Parasitol. 2019;207:107774.
Novato, T. L. P., Marchesini, P., Muniz, N., Prata, M. C. A., Furlong, J., Vilela, F. M. P., Daemon, E., Maturano, R., & Monteiro, C. (2019). Evaluation of synergism and development of a formulation with thymol, carvacrol and eugenol for Rhipicephalus microplus control. Experimental Parasitology, 207, 107774. https://doi.org/10.1016/j.exppara.2019.107774
Novato TLP, et al. Evaluation of Synergism and Development of a Formulation With Thymol, Carvacrol and Eugenol for Rhipicephalus Microplus Control. Exp Parasitol. 2019;207:107774. PubMed PMID: 31614118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of synergism and development of a formulation with thymol, carvacrol and eugenol for Rhipicephalus microplus control. AU - Novato,Tatiane Lopes Pinheiro, AU - Marchesini,Paula, AU - Muniz,Natália, AU - Prata,Márcia Cristina de Azevedo, AU - Furlong,John, AU - Vilela,Fernanda Maria Pinto, AU - Daemon,Erik, AU - Maturano,Ralph, AU - Monteiro,Caio, Y1 - 2019/10/12/ PY - 2019/05/22/received PY - 2019/08/20/revised PY - 2019/10/09/accepted PY - 2019/10/16/pubmed PY - 2019/12/4/medline PY - 2019/10/16/entrez KW - Botanical acaricide KW - Cattle tick KW - Essential oil KW - Synergism SP - 107774 EP - 107774 JF - Experimental parasitology JO - Exp. Parasitol. VL - 207 N2 - The acaricidal activity of combinations of thymol, carvacrol and eugenol was evaluated on larvae and engorged females of the cattle tick Rhipicephalus microplus. The first step assessed the compounds separately, in concentrations of 3.125, 6.25, 12.5 and 25 mg/mL. Then tests were performed with the compounds combined in the ratio of 1:1 at concentrations of 3.125 and 6.25 mg/mL, along with the control group treated with the solvent (3% DMSO). In the second step, combinations were tested incorporated in a formulation at the concentration de 6.25 mg/mL, using the larval packet and adult immersion tests. The associations carvacrol + thymol (3.125 mg/mL), carvacrol + eugenol and thymol + eugenol (6.25 mg/mL) presented synergism, while the other associations had an additive effect. In the experiments with formulation, all combinations caused 100% larval mortality, but the efficacy was under 15% against engorged females. Therefore, the combinations of thymol + carvacrol (3.125 mg/mL) as well as carvacrol + eugenol and eugenol + thymol (6.25 mg/mL) had a synergistic effect on engorged females, but when incorporated in the formulation, the acaricide activity was strong against larvae but weak against engorged females. SN - 1090-2449 UR - https://www.unboundmedicine.com/medline/citation/31614118/Evaluation_of_synergism_and_development_of_a_formulation_with_thymol_carvacrol_and_eugenol_for_Rhipicephalus_microplus_control_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4894(19)30213-9 DB - PRIME DP - Unbound Medicine ER -