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The use of adenosine to inhibit oocyte meiotic resumption in Bos taurus during pre-IVM and its potential to improve oocyte competence.
Theriogenology 2019; 142:207-215T

Abstract

One of the major challenges of artificial reproductive technologies is to develop new methods for producing greater numbers of embryos. An oocyte fosters the ability to develop into an embryo before oocyte meiotic resumption. The aim of the present study was to assess the effect of adenosine (ADO), a purine nucleoside found in follicular fluid, on the inhibition of oocyte meiotic resumption and the production of blastocysts. The results showed the efficacy of ADO to inhibit oocyte meiotic resumption. The use of ADO (3 mM) during a pre-in vitro maturation (pre-IVM) culture period of 6 h resulted in a significant increase (p < 0.05) of blastocysts compared to control conditions with no pre-IVM culture period. No effect on the percentage of cleavage was observed. The effect of adenosine on blastocyst yield was time- and concentration-dependent with an optimum effect at 3 mM for 6 h. Supplementing the ADO pre-IVM culture medium with estradiol, follicle-stimulating hormone, progesterone, epidermal growth factor, insulin-like growth factor-2 or reelin did not improve the blastocyst yield. Transcriptional analyses of ADO-treated cumulus cells revealed that NRP1, RELN, MAN1A1, THRA and GATM were up-regulated. Finally, bioinformatic analysis identified mitochondrial function as the top canonical pathway affected by ADO. This opens up new opportunities for further investigations.

Authors+Show Affiliations

Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Département des Sciences Animales, Faculté des Sciences de L'agriculture et de L'alimentation, Université Laval, Québec, Québec, G1V 0A6, Canada.L'Alliance Boviteq Inc, 19320 Grand Rang St-François, Saint-Hyacinthe, Québec, J2T 5H1, Canada.L'Alliance Boviteq Inc, 19320 Grand Rang St-François, Saint-Hyacinthe, Québec, J2T 5H1, Canada.Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Département des Sciences Animales, Faculté des Sciences de L'agriculture et de L'alimentation, Université Laval, Québec, Québec, G1V 0A6, Canada.Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Département des Sciences Animales, Faculté des Sciences de L'agriculture et de L'alimentation, Université Laval, Québec, Québec, G1V 0A6, Canada. Electronic address: Francois.Richard@fsaa.ulaval.ca.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31614287

Citation

Caballero, Julieta, et al. "The Use of Adenosine to Inhibit Oocyte Meiotic Resumption in Bos Taurus During pre-IVM and Its Potential to Improve Oocyte Competence." Theriogenology, vol. 142, 2019, pp. 207-215.
Caballero J, Blondin P, Vigneault C, et al. The use of adenosine to inhibit oocyte meiotic resumption in Bos taurus during pre-IVM and its potential to improve oocyte competence. Theriogenology. 2019;142:207-215.
Caballero, J., Blondin, P., Vigneault, C., Sirard, M. A., & Richard, F. J. (2019). The use of adenosine to inhibit oocyte meiotic resumption in Bos taurus during pre-IVM and its potential to improve oocyte competence. Theriogenology, 142, pp. 207-215. doi:10.1016/j.theriogenology.2019.10.001.
Caballero J, et al. The Use of Adenosine to Inhibit Oocyte Meiotic Resumption in Bos Taurus During pre-IVM and Its Potential to Improve Oocyte Competence. Theriogenology. 2019 Oct 7;142:207-215. PubMed PMID: 31614287.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of adenosine to inhibit oocyte meiotic resumption in Bos taurus during pre-IVM and its potential to improve oocyte competence. AU - Caballero,Julieta, AU - Blondin,Patrick, AU - Vigneault,Christian, AU - Sirard,Marc-André, AU - Richard,François J, Y1 - 2019/10/07/ PY - 2019/05/28/received PY - 2019/09/10/revised PY - 2019/10/03/accepted PY - 2019/10/16/pubmed PY - 2019/10/16/medline PY - 2019/10/16/entrez KW - Bovine KW - Embryo KW - Genomics KW - Meiotic arrest KW - Oocyte SP - 207 EP - 215 JF - Theriogenology JO - Theriogenology VL - 142 N2 - One of the major challenges of artificial reproductive technologies is to develop new methods for producing greater numbers of embryos. An oocyte fosters the ability to develop into an embryo before oocyte meiotic resumption. The aim of the present study was to assess the effect of adenosine (ADO), a purine nucleoside found in follicular fluid, on the inhibition of oocyte meiotic resumption and the production of blastocysts. The results showed the efficacy of ADO to inhibit oocyte meiotic resumption. The use of ADO (3 mM) during a pre-in vitro maturation (pre-IVM) culture period of 6 h resulted in a significant increase (p < 0.05) of blastocysts compared to control conditions with no pre-IVM culture period. No effect on the percentage of cleavage was observed. The effect of adenosine on blastocyst yield was time- and concentration-dependent with an optimum effect at 3 mM for 6 h. Supplementing the ADO pre-IVM culture medium with estradiol, follicle-stimulating hormone, progesterone, epidermal growth factor, insulin-like growth factor-2 or reelin did not improve the blastocyst yield. Transcriptional analyses of ADO-treated cumulus cells revealed that NRP1, RELN, MAN1A1, THRA and GATM were up-regulated. Finally, bioinformatic analysis identified mitochondrial function as the top canonical pathway affected by ADO. This opens up new opportunities for further investigations. SN - 1879-3231 UR - https://www.unboundmedicine.com/medline/citation/31614287/The_use_of_adenosine_to_inhibit_oocyte_meiotic_resumption_in_Bos_taurus_during_pre-IVM_and_its_potential_to_improve_oocyte_competence L2 - https://linkinghub.elsevier.com/retrieve/pii/S0093-691X(19)30448-0 DB - PRIME DP - Unbound Medicine ER -