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Effects of a Flavonoid-Rich Extract from Citrus sinensis Juice on a Diet-Induced Obese Zebrafish.
Int J Mol Sci 2019; 20(20)IJ

Abstract

BACKGROUND

Obesity is a pathological condition that has reached epidemic proportions; hence, it is necessary to find novel strategies aimed at fighting this disease. The present study was designed to evaluate the effect of a flavonoid-rich extract of orange (Citrus sinensis) juice (OJe) in diet-induced obese zebrafish.

METHODS

Adult zebrafish were divided into four diet groups: (i) normally fed (NF); (ii) overfed (OF); (iii) NF supplemented with OJe (5 mL/L in fish water; NF + OJe); and (iv) OF supplemented with OJe (OF + OJe). Each week, body weight (BW) and body mass index (BMI) were measured, and, at the end of the fifth week, euthanized zebrafish were processed for both microscopic evaluations and qPCR analyses.

RESULTS

In OF zebrafish, OJe significantly decreased both BW and BMI values and lowered the visceral adipose tissue, while it had little effect in the NF group. Moreover, it significantly reduced adipocyte cell size in both NF and OF groups in both visceral and subcutaneous adipose tissues, as well as their number in OF fish. Finally, OJe modulated some obesity-related genes, such as leptin A, ghrelin, orexin, pro-opiomelanocortin (POMC), and neuropeptide Y (NPY), in both gut and brain.

CONCLUSION

This study adds new insights into the anti-obesity properties of orange juice and its flavonoids, suggesting their role as weight management agents through a lipolytic action linked to a restoration of metabolism-regulating gene expression.

Authors+Show Affiliations

Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy. gmontalbano@unime.it.Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy. manuela.mania87@gmail.com.Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy. mcguerrera@unime.it.Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy. laurar@unime.it.Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy. abbatef@unime.it.Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy. mblevanti@unime.it.Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98122 Messina, Italy. amaugeri@unime.it.Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy. agermana@unime.it.Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98122 Messina, Italy. mnavarra@unime.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31619003

Citation

Montalbano, Giuseppe, et al. "Effects of a Flavonoid-Rich Extract From Citrus Sinensis Juice On a Diet-Induced Obese Zebrafish." International Journal of Molecular Sciences, vol. 20, no. 20, 2019.
Montalbano G, Mania M, Guerrera MC, et al. Effects of a Flavonoid-Rich Extract from Citrus sinensis Juice on a Diet-Induced Obese Zebrafish. Int J Mol Sci. 2019;20(20).
Montalbano, G., Mania, M., Guerrera, M. C., Laurà, R., Abbate, F., Levanti, M., ... Navarra, M. (2019). Effects of a Flavonoid-Rich Extract from Citrus sinensis Juice on a Diet-Induced Obese Zebrafish. International Journal of Molecular Sciences, 20(20), doi:10.3390/ijms20205116.
Montalbano G, et al. Effects of a Flavonoid-Rich Extract From Citrus Sinensis Juice On a Diet-Induced Obese Zebrafish. Int J Mol Sci. 2019 Oct 15;20(20) PubMed PMID: 31619003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of a Flavonoid-Rich Extract from Citrus sinensis Juice on a Diet-Induced Obese Zebrafish. AU - Montalbano,Giuseppe, AU - Mania,Manuela, AU - Guerrera,Maria Cristina, AU - Laurà,Rosaria, AU - Abbate,Francesco, AU - Levanti,Maria, AU - Maugeri,Alessandro, AU - Germanà,Antonino, AU - Navarra,Michele, Y1 - 2019/10/15/ PY - 2019/08/01/received PY - 2019/10/10/revised PY - 2019/10/11/accepted PY - 2019/10/18/entrez KW - Citrus sinensis KW - diet-induced obesity KW - flavonoids KW - lipolysis KW - orange juice KW - zebrafish JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 20 N2 - BACKGROUND: Obesity is a pathological condition that has reached epidemic proportions; hence, it is necessary to find novel strategies aimed at fighting this disease. The present study was designed to evaluate the effect of a flavonoid-rich extract of orange (Citrus sinensis) juice (OJe) in diet-induced obese zebrafish. METHODS: Adult zebrafish were divided into four diet groups: (i) normally fed (NF); (ii) overfed (OF); (iii) NF supplemented with OJe (5 mL/L in fish water; NF + OJe); and (iv) OF supplemented with OJe (OF + OJe). Each week, body weight (BW) and body mass index (BMI) were measured, and, at the end of the fifth week, euthanized zebrafish were processed for both microscopic evaluations and qPCR analyses. RESULTS: In OF zebrafish, OJe significantly decreased both BW and BMI values and lowered the visceral adipose tissue, while it had little effect in the NF group. Moreover, it significantly reduced adipocyte cell size in both NF and OF groups in both visceral and subcutaneous adipose tissues, as well as their number in OF fish. Finally, OJe modulated some obesity-related genes, such as leptin A, ghrelin, orexin, pro-opiomelanocortin (POMC), and neuropeptide Y (NPY), in both gut and brain. CONCLUSION: This study adds new insights into the anti-obesity properties of orange juice and its flavonoids, suggesting their role as weight management agents through a lipolytic action linked to a restoration of metabolism-regulating gene expression. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31619003/Effects_of_a_Flavonoid-Rich_Extract_from_Citrus_sinensis_Juice_on_a_Diet-Induced_Obese_Zebrafish DB - PRIME DP - Unbound Medicine ER -