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Identification of quantitative trait loci for kernel traits in a wheat cultivar Chuannong16.
BMC Genet. 2019 10 16; 20(1):77.BG

Abstract

BACKGROUND

Kernel length (KL), kernel width (KW) and thousand-kernel weight (TKW) are key agronomic traits in wheat breeding. Chuannong16 ('CN16') is a commercial cultivar with significantly longer kernels than the line '20828'. To identify and characterize potential alleles from CN16 controlling KL, the previously developed recombinant inbred line (RIL) population derived from the cross '20828' × 'CN16' and the genetic map constructed by the Wheat55K SNP array and SSR markers were used to perform quantitative trait locus/loci (QTL) analyses for kernel traits.

RESULTS

A total of 11 putative QTL associated with kernel traits were identified and they were located on chromosomes 1A (2 QTL), 2B (2 QTL), 2D (3 QTL), 3D, 4A, 6A, and 7A, respectively. Among them, three major QTL, QKL.sicau-2D, QKW.sicau-2D and QTKW.sicau-2D, controlling KL, KW and TKW, respectively, were detected in three different environments. Respectively, they explained 10.88-18.85%, 17.21-21.49% and 10.01-23.20% of the phenotypic variance. Further, they were genetically mapped in the same interval on chromosome 2DS. A previously developed kompetitive allele-specific PCR (KASP) marker KASP-AX-94721936 was integrated in the genetic map and QTL re-mapping finally located the three major QTL in a 1- cM region flanked by AX-111096297 and KASP-AX-94721936. Another two co-located QTL intervals for KL and TKW were also identified. A few predicted genes involved in regulation of kernel growth and development were identified in the intervals of these identified QTL. Significant relationships between kernel traits and spikelet number per spike and anthesis date were detected and discussed.

CONCLUSIONS

Three major and stably expressed QTL associated with KL, KW, and TKW were identified. A KASP marker tightly linked to these three major QTL was integrated. These findings provide information for subsequent fine mapping and cloning the three co-localized major QTL for kernel traits.

Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. jianma@sicau.edu.cn. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China. jianma@sicau.edu.cn.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. lanxiujin@163.com. China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China. lanxiujin@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31619163

Citation

Ma, Jian, et al. "Identification of Quantitative Trait Loci for Kernel Traits in a Wheat Cultivar Chuannong16." BMC Genetics, vol. 20, no. 1, 2019, p. 77.
Ma J, Zhang H, Li S, et al. Identification of quantitative trait loci for kernel traits in a wheat cultivar Chuannong16. BMC Genet. 2019;20(1):77.
Ma, J., Zhang, H., Li, S., Zou, Y., Li, T., Liu, J., Ding, P., Mu, Y., Tang, H., Deng, M., Liu, Y., Jiang, Q., Chen, G., Kang, H., Li, W., Pu, Z., Wei, Y., Zheng, Y., & Lan, X. (2019). Identification of quantitative trait loci for kernel traits in a wheat cultivar Chuannong16. BMC Genetics, 20(1), 77. https://doi.org/10.1186/s12863-019-0782-4
Ma J, et al. Identification of Quantitative Trait Loci for Kernel Traits in a Wheat Cultivar Chuannong16. BMC Genet. 2019 10 16;20(1):77. PubMed PMID: 31619163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of quantitative trait loci for kernel traits in a wheat cultivar Chuannong16. AU - Ma,Jian, AU - Zhang,Han, AU - Li,Shuiqin, AU - Zou,Yaya, AU - Li,Ting, AU - Liu,Jiajun, AU - Ding,Puyang, AU - Mu,Yang, AU - Tang,Huaping, AU - Deng,Mei, AU - Liu,Yaxi, AU - Jiang,Qiantao, AU - Chen,Guoyue, AU - Kang,Houyang, AU - Li,Wei, AU - Pu,Zhien, AU - Wei,Yuming, AU - Zheng,Youliang, AU - Lan,Xiujin, Y1 - 2019/10/16/ PY - 2019/09/09/received PY - 2019/09/26/accepted PY - 2019/10/18/entrez PY - 2019/10/18/pubmed PY - 2020/5/19/medline KW - 55 K SNP array KW - Common wheat KW - KASP marker KW - Kernel traits KW - QTL SP - 77 EP - 77 JF - BMC genetics JO - BMC Genet VL - 20 IS - 1 N2 - BACKGROUND: Kernel length (KL), kernel width (KW) and thousand-kernel weight (TKW) are key agronomic traits in wheat breeding. Chuannong16 ('CN16') is a commercial cultivar with significantly longer kernels than the line '20828'. To identify and characterize potential alleles from CN16 controlling KL, the previously developed recombinant inbred line (RIL) population derived from the cross '20828' × 'CN16' and the genetic map constructed by the Wheat55K SNP array and SSR markers were used to perform quantitative trait locus/loci (QTL) analyses for kernel traits. RESULTS: A total of 11 putative QTL associated with kernel traits were identified and they were located on chromosomes 1A (2 QTL), 2B (2 QTL), 2D (3 QTL), 3D, 4A, 6A, and 7A, respectively. Among them, three major QTL, QKL.sicau-2D, QKW.sicau-2D and QTKW.sicau-2D, controlling KL, KW and TKW, respectively, were detected in three different environments. Respectively, they explained 10.88-18.85%, 17.21-21.49% and 10.01-23.20% of the phenotypic variance. Further, they were genetically mapped in the same interval on chromosome 2DS. A previously developed kompetitive allele-specific PCR (KASP) marker KASP-AX-94721936 was integrated in the genetic map and QTL re-mapping finally located the three major QTL in a 1- cM region flanked by AX-111096297 and KASP-AX-94721936. Another two co-located QTL intervals for KL and TKW were also identified. A few predicted genes involved in regulation of kernel growth and development were identified in the intervals of these identified QTL. Significant relationships between kernel traits and spikelet number per spike and anthesis date were detected and discussed. CONCLUSIONS: Three major and stably expressed QTL associated with KL, KW, and TKW were identified. A KASP marker tightly linked to these three major QTL was integrated. These findings provide information for subsequent fine mapping and cloning the three co-localized major QTL for kernel traits. SN - 1471-2156 UR - https://www.unboundmedicine.com/medline/citation/31619163/Identification_of_quantitative_trait_loci_for_kernel_traits_in_a_wheat_cultivar_Chuannong16_ L2 - https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-019-0782-4 DB - PRIME DP - Unbound Medicine ER -