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Genetic and phenotypic characterization of recently discovered enterovirus D type 111.
PLoS Negl Trop Dis 2019; 13(10):e0007797PN

Abstract

Members of the species Enterovirus D (EV-D) remain poorly studied. The two first EV-D types (EV-D68 and EV-D70) have regularly caused outbreaks in humans since their discovery five decades ago but have been neglected until the recent occurrence of severe respiratory diseases due to EV-D68. The three other known EV-D types (EV-D94, EV-D111 and EV-D120) were discovered in the 2000s-2010s in Africa and have never been observed elsewhere. One strain of EV-D111 and all known EV-D120s were detected in stool samples of wild non-human primates, suggesting that these viruses could be zoonotic viruses. To date, EV-D111s are only known through partial genetic sequences of the few strains that have been identified so far. In an attempt to bring new pieces to the puzzle, we genetically characterized four EV-D111 strains (among the seven that have been reported until now). We observed that the EV-D111 strains from human samples and the unique simian EV-D111 strain were not phylogenetically distinct, thus suggesting a recent zoonotic transmission. We also discovered evidences of probable intertypic genetic recombination events between EV-D111s and EV-D94s. As recombination can only happen in co-infected cells, this suggests that EV-D94s and EV-D111s share common replication sites in the infected hosts. These sites could be located in the gut since the phenotypic analysis we performed showed that, contrary to EV-D68s and like EV-D94s, EV-D111s are resistant to acid pHs. We also found that EV-D111s induce strong cytopathic effects on L20B cells, a cell line routinely used to specifically detect polioviruses. An active circulation of EV-D111s among humans could then induce a high number of false-positive detection of polioviruses, which could be particularly problematic in Central Africa, where EV-D111 circulates and which is a key region for poliovirus eradication.

Authors+Show Affiliations

Virology Service-Centre Pasteur of Cameroon-Yaounde, Cameroon.Institut Pasteur-Unité de biologie des virus entériques-Paris, France. WHO Collaborating Centre for Enteroviruses and Viral Vaccines-Paris, France.Enteric Viruses and Measles Laboratory-Institut Pasteur de Bangui-Bangui, Central African Republic.Virology Service-Centre Pasteur of Cameroon-Yaounde, Cameroon.Virology Service-Centre Pasteur of Cameroon-Yaounde, Cameroon.Institut Pasteur-Unité de biologie des virus entériques-Paris, France. WHO Collaborating Centre for Enteroviruses and Viral Vaccines-Paris, France.Enteric Viruses and Measles Laboratory-Institut Pasteur de Bangui-Bangui, Central African Republic.Institut Pasteur-Unité de biologie des virus entériques-Paris, France. WHO Collaborating Centre for Enteroviruses and Viral Vaccines-Paris, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31622358

Citation

Sadeuh-Mba, Serge Alain, et al. "Genetic and Phenotypic Characterization of Recently Discovered Enterovirus D Type 111." PLoS Neglected Tropical Diseases, vol. 13, no. 10, 2019, pp. e0007797.
Sadeuh-Mba SA, Joffret ML, Mazitchi A, et al. Genetic and phenotypic characterization of recently discovered enterovirus D type 111. PLoS Negl Trop Dis. 2019;13(10):e0007797.
Sadeuh-Mba, S. A., Joffret, M. L., Mazitchi, A., Endegue-Zanga, M. C., Njouom, R., Delpeyroux, F., ... Bessaud, M. (2019). Genetic and phenotypic characterization of recently discovered enterovirus D type 111. PLoS Neglected Tropical Diseases, 13(10), pp. e0007797. doi:10.1371/journal.pntd.0007797.
Sadeuh-Mba SA, et al. Genetic and Phenotypic Characterization of Recently Discovered Enterovirus D Type 111. PLoS Negl Trop Dis. 2019;13(10):e0007797. PubMed PMID: 31622358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic and phenotypic characterization of recently discovered enterovirus D type 111. AU - Sadeuh-Mba,Serge Alain, AU - Joffret,Marie-Line, AU - Mazitchi,Arthur, AU - Endegue-Zanga,Marie-Claire, AU - Njouom,Richard, AU - Delpeyroux,Francis, AU - Gouandjika-Vasilache,Ionela, AU - Bessaud,Maël, Y1 - 2019/10/17/ PY - 2018/12/12/received PY - 2019/09/18/accepted PY - 2019/10/29/revised PY - 2019/10/18/pubmed PY - 2019/10/18/medline PY - 2019/10/18/entrez SP - e0007797 EP - e0007797 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 13 IS - 10 N2 - Members of the species Enterovirus D (EV-D) remain poorly studied. The two first EV-D types (EV-D68 and EV-D70) have regularly caused outbreaks in humans since their discovery five decades ago but have been neglected until the recent occurrence of severe respiratory diseases due to EV-D68. The three other known EV-D types (EV-D94, EV-D111 and EV-D120) were discovered in the 2000s-2010s in Africa and have never been observed elsewhere. One strain of EV-D111 and all known EV-D120s were detected in stool samples of wild non-human primates, suggesting that these viruses could be zoonotic viruses. To date, EV-D111s are only known through partial genetic sequences of the few strains that have been identified so far. In an attempt to bring new pieces to the puzzle, we genetically characterized four EV-D111 strains (among the seven that have been reported until now). We observed that the EV-D111 strains from human samples and the unique simian EV-D111 strain were not phylogenetically distinct, thus suggesting a recent zoonotic transmission. We also discovered evidences of probable intertypic genetic recombination events between EV-D111s and EV-D94s. As recombination can only happen in co-infected cells, this suggests that EV-D94s and EV-D111s share common replication sites in the infected hosts. These sites could be located in the gut since the phenotypic analysis we performed showed that, contrary to EV-D68s and like EV-D94s, EV-D111s are resistant to acid pHs. We also found that EV-D111s induce strong cytopathic effects on L20B cells, a cell line routinely used to specifically detect polioviruses. An active circulation of EV-D111s among humans could then induce a high number of false-positive detection of polioviruses, which could be particularly problematic in Central Africa, where EV-D111 circulates and which is a key region for poliovirus eradication. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/31622358/Genetic_and_phenotypic_characterization_of_recently_discovered_enterovirus_D_type_111 L2 - http://dx.plos.org/10.1371/journal.pntd.0007797 DB - PRIME DP - Unbound Medicine ER -