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Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus.
J Chromatogr A 2019; :460578JC

Abstract

The macroporous microspheres with core-shell structure, based on a copolymer of 4-Vinylbenzyl chloride, glycidyl methacrylate, and ethylene glycol dimethacrylate, were fabricated through atom transfer radical polymerization suspension polymerization. The microspheres showed 100-200 nm pores in shell and 500-900 nm pores in core. The shell was hydrophilic modified through grafting of poly(N-hydroxyethyl acrylamide) onto the shell surface for reducing adsorption of proteins. The core was coupled with a ligand of poly(ethylene imine) that could bind the proteins. Feedstock of avian influenza virus could be purified on these modified microspheres through negative chromatography. Avian influenza virus cannot enter the core and was recovered from the flow-through, while other proteins with negative charges were able to penetrate into the core and bind to the poly(ethylene imine) ligands. The dynamic binding capacity of proteins was higher on this medium (61 mg/mL) than the commercially available resin (12 mg/mL, Capto Core 700).

Authors+Show Affiliations

Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China.Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China. Electronic address: zhangrongyue@bipt.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31623846

Citation

An, Ning, et al. "Fabrication of Macroporous Microspheres With Core-shell Structure for Negative Chromatography Purification of Virus." Journal of Chromatography. A, 2019, p. 460578.
An N, Gong P, Hou H, et al. Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus. J Chromatogr A. 2019.
An, N., Gong, P., Hou, H., Chi, W., Jin, H., Zhao, L., ... Zhang, R. (2019). Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus. Journal of Chromatography. A, p. 460578. doi:10.1016/j.chroma.2019.460578.
An N, et al. Fabrication of Macroporous Microspheres With Core-shell Structure for Negative Chromatography Purification of Virus. J Chromatogr A. 2019 Sep 27;460578. PubMed PMID: 31623846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus. AU - An,Ning, AU - Gong,Pisheng, AU - Hou,Henglei, AU - Chi,Weiya, AU - Jin,Haibo, AU - Zhao,Lan, AU - Tan,Qiqi, AU - Tang,Xu, AU - Wang,Fei, AU - Jin,Hongchao, AU - Zhang,Rongyue, Y1 - 2019/09/27/ PY - 2019/07/12/received PY - 2019/09/25/revised PY - 2019/09/26/accepted PY - 2019/10/19/entrez KW - Avian influenza virus KW - Macroporous microsphere with core-shell structure KW - Negative chromatography KW - Purification of virus SP - 460578 EP - 460578 JF - Journal of chromatography. A JO - J Chromatogr A N2 - The macroporous microspheres with core-shell structure, based on a copolymer of 4-Vinylbenzyl chloride, glycidyl methacrylate, and ethylene glycol dimethacrylate, were fabricated through atom transfer radical polymerization suspension polymerization. The microspheres showed 100-200 nm pores in shell and 500-900 nm pores in core. The shell was hydrophilic modified through grafting of poly(N-hydroxyethyl acrylamide) onto the shell surface for reducing adsorption of proteins. The core was coupled with a ligand of poly(ethylene imine) that could bind the proteins. Feedstock of avian influenza virus could be purified on these modified microspheres through negative chromatography. Avian influenza virus cannot enter the core and was recovered from the flow-through, while other proteins with negative charges were able to penetrate into the core and bind to the poly(ethylene imine) ligands. The dynamic binding capacity of proteins was higher on this medium (61 mg/mL) than the commercially available resin (12 mg/mL, Capto Core 700). SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/31623846/Fabrication_of_macroporous_microspheres_with_core-shell_structure_for_negative_chromatography_purification_of_virus L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(19)30972-0 DB - PRIME DP - Unbound Medicine ER -