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Larval development and proteolytic activity of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) exposed to different soybean protease inhibitors.
Arch Insect Biochem Physiol. 2020 Jan; 103(1):e21637.AI

Abstract

Anticarsia gemmatalis represents a relevant factor for lowering soybean and other legume crop productivities. Protease inhibitors affect protein degradation and reduce the availability of amino acids, impairing the development and survival of insect pests. To evaluate the possible use of proteinaceous protease inhibitors in the management of this pest, the activities of midgut proteases and the growth and development of A. gemmatalis larvae exposed to soybean Bowman-Birk trypsin-chymotrypsin inhibitor (SBBI) and soybean Kunitz trypsin inhibitor (SKTI) were determined. The survival curves obtained using Kaplan-Meier estimators indicated that SKTI and SBBI stimulated larval survival. However, the development of A. gemmatalis was delayed, and prepupal weight decreased in the presence of both inhibitors. The results showed that SKTI and SBBI inhibited the trypsin-like and total proteolytic activities of larvae on the 12th day after eclosion. On the 15th day after eclosion, larvae exposed to SKTI increased the activities of trypsin and total proteases. Although SKTI and SBBI did not affect the survival of the insect, they had effects on midgut proteases in a stage wherein A. gemmatalis fed voraciously, increased the larval cycle, and decreased prepupal weight. These findings provide baseline information about the potential of proteinaceous protease inhibitors to manage the velvetbean caterpillar, avoiding chemical pesticides.

Authors+Show Affiliations

Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Brazil.Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Brazil.Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Brazil.Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Brazil.Departamento de Engenharia de Biossistemas, Campus Dom Bosco, Universidade Federal de São João Del-Rei, São João Del-Rei, Brazil.Departamento de Química, Universidade Federal de Viçosa, Viçosa, Brazil.Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31625209

Citation

Mendonça, Eduardo G., et al. "Larval Development and Proteolytic Activity of Anticarsia Gemmatalis Hübner (Lepidoptera: Noctuidae) Exposed to Different Soybean Protease Inhibitors." Archives of Insect Biochemistry and Physiology, vol. 103, no. 1, 2020, pp. e21637.
Mendonça EG, de Almeida Barros R, Cordeiro G, et al. Larval development and proteolytic activity of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) exposed to different soybean protease inhibitors. Arch Insect Biochem Physiol. 2020;103(1):e21637.
Mendonça, E. G., de Almeida Barros, R., Cordeiro, G., da Silva, C. R., Campos, W. G., de Oliveira, J. A., & de Almeida Oliveira, M. G. (2020). Larval development and proteolytic activity of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) exposed to different soybean protease inhibitors. Archives of Insect Biochemistry and Physiology, 103(1), e21637. https://doi.org/10.1002/arch.21637
Mendonça EG, et al. Larval Development and Proteolytic Activity of Anticarsia Gemmatalis Hübner (Lepidoptera: Noctuidae) Exposed to Different Soybean Protease Inhibitors. Arch Insect Biochem Physiol. 2020;103(1):e21637. PubMed PMID: 31625209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Larval development and proteolytic activity of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) exposed to different soybean protease inhibitors. AU - Mendonça,Eduardo G, AU - de Almeida Barros,Rafael, AU - Cordeiro,Gláucia, AU - da Silva,Carolina R, AU - Campos,Welligton G, AU - de Oliveira,Joel A, AU - de Almeida Oliveira,Maria G, Y1 - 2019/10/17/ PY - 2019/08/08/received PY - 2019/09/16/revised PY - 2019/10/01/accepted PY - 2019/10/19/pubmed PY - 2020/1/9/medline PY - 2019/10/19/entrez KW - biotic stress KW - insect attack KW - insect performance KW - protease inhibitory SP - e21637 EP - e21637 JF - Archives of insect biochemistry and physiology JO - Arch Insect Biochem Physiol VL - 103 IS - 1 N2 - Anticarsia gemmatalis represents a relevant factor for lowering soybean and other legume crop productivities. Protease inhibitors affect protein degradation and reduce the availability of amino acids, impairing the development and survival of insect pests. To evaluate the possible use of proteinaceous protease inhibitors in the management of this pest, the activities of midgut proteases and the growth and development of A. gemmatalis larvae exposed to soybean Bowman-Birk trypsin-chymotrypsin inhibitor (SBBI) and soybean Kunitz trypsin inhibitor (SKTI) were determined. The survival curves obtained using Kaplan-Meier estimators indicated that SKTI and SBBI stimulated larval survival. However, the development of A. gemmatalis was delayed, and prepupal weight decreased in the presence of both inhibitors. The results showed that SKTI and SBBI inhibited the trypsin-like and total proteolytic activities of larvae on the 12th day after eclosion. On the 15th day after eclosion, larvae exposed to SKTI increased the activities of trypsin and total proteases. Although SKTI and SBBI did not affect the survival of the insect, they had effects on midgut proteases in a stage wherein A. gemmatalis fed voraciously, increased the larval cycle, and decreased prepupal weight. These findings provide baseline information about the potential of proteinaceous protease inhibitors to manage the velvetbean caterpillar, avoiding chemical pesticides. SN - 1520-6327 UR - https://www.unboundmedicine.com/medline/citation/31625209/Larval_development_and_proteolytic_activity_of_Anticarsia_gemmatalis_Hübner__Lepidoptera:_Noctuidae__exposed_to_different_soybean_protease_inhibitors_ L2 - https://doi.org/10.1002/arch.21637 DB - PRIME DP - Unbound Medicine ER -